Proliferative and phenotypical characteristics of human adipose tissue-derived stem cells: Comparison of Ficoll gradient centrifugation and red blood cell lysis buffer treatment purification methods

Cytotherapy

Volumn 16, Issue 9, 2014, Pages 1220-1228

  Najar, Mehdi ^a   Rodrigues, Robim M ^b   Buyl, Karolien ^b   Branson, Steven ^b   Vanhaecke, Tamara ^b   Lagneaux, Laurence ^a   Rogiers, Vera ^b   De Kock, Joery ^b  

^a JULES BORDET INSTITUTE   (Belgium)

^b VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Adipose tissue; Adult stem cell; Density gradient centrifugation; Ficoll; Mesenchymal stromal cell; Red blood cell lysis; Stromal vascular fraction

Indexed keywords

5' NUCLEOTIDASE; CD14 ANTIGEN; CD19 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN; ENDOGLIN; HEME OXYGENASE 1; HLA DR ANTIGEN; INTERCELLULAR ADHESION MOLECULE 1; LEUKEMIA INHIBITORY FACTOR; PROSTAGLANDIN SYNTHASE; SCATTER FACTOR; THY 1 ANTIGEN; VASCULAR CELL ADHESION MOLECULE 1; BUFFER; FICOLL;

ADIPOSE DERIVED STEM CELL; ADULT; ARTICLE; CELL ADHESION; CELL DIFFERENTIATION; CELL EXPANSION; CELL GROWTH; CELL ISOLATION; CELL POPULATION; CELL PROLIFERATION; CELL STRUCTURE; CLINICAL ARTICLE; DENSITY GRADIENT CENTRIFUGATION; FEMALE; FICOLL GRADIENT CENTRIFUGATION; FLOW CYTOMETRY; HEMOLYSIS; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; INTERMETHOD COMPARISON; MALE; MESENCHYMAL STROMA CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PURIFICATION; ADIPOSE TISSUE; APOPTOSIS; CELL CULTURE; CELL SEPARATION; CHEMISTRY; COMPARATIVE STUDY; CYTOLOGY; ERYTHROCYTE; PHENOTYPE; PHYSIOLOGY; PLURIPOTENT STEM CELL; PROCEDURES; STEM CELL NICHE; STEM CELL TRANSPLANTATION;

ADIPOSE TISSUE; APOPTOSIS; BUFFERS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; ERYTHROCYTES; FICOLL; HUMANS; PHENOTYPE; PLURIPOTENT STEM CELLS; STEM CELL NICHE; STEM CELL TRANSPLANTATION;

EID: 84905574289     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.1016/j.jcyt.2014.05.021     Document Type: Article

References (33)

1. Mimeault M., Batra S.K. Recent progress on tissue-resident adult stem cell biology and their therapeutic implications. Stem Cell Rev 2008, 4:27-49.
2. Li L., Clevers H. Coexistence of quiescent and active adult stem cells in mammals. Science 2010, 327:542-545.
3. Dominici M., Le Blanc K., Mueller I., Slaper-Cortenbach I., Marini F., Krause D., et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006, 8:315-317.
4. Kastrinaki M.C., Andreakou I., Charbord P., Papadaki H.A. Isolation of human bone marrow mesenchymal stem cells using different membrane markers: comparison of colony/cloning efficiency, differentiation potential, and molecular profile. Tissue Eng Part C Methods 2008, 14:333-339.
5. Najimi M., Khuu D.N., Lysy P.A., Jazouli N., Abarca J., Sempoux C., et al. Adult-derived human liver mesenchymal-like cells as a potential progenitor reservoir of hepatocytes?. Cell Transplant 2007, 16:717-728.
6. Zuk P.A., Zhu M., Mizuno H., Huang J., Futrell J.W., Katz A.J., et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 2001, 7:211-228.
7. Buyl K., De Kock J., Najar M., Lagneaux L., Branson S., Rogiers V., et al. Characterization of hepatic markers in human Wharton's Jelly-derived mesenchymal stem cells. Toxicol In Vitro 2014, 28:113-119.
8. De Bruyn C., Najar M., Raicevic G., Meuleman N., Pieters K., Stamatopoulos B., et al. A rapid, simple, and reproducible method for the isolation of mesenchymal stromal cells from Wharton's jelly without enzymatic treatment. Stem Cells Dev 2011, 20(3):547-557.
9. Najar M., Raicevic G., Boufker H.I., Fayyad-Kazan H., De Bruyn C., Meuleman N., et al. Adipose-tissue-derived and Wharton's jelly-derived mesenchymal stromal cells suppress lymphocyte responses by secreting leukemia inhibitory factor. Tissue Eng Part A 2010, 16:3537-3546.
10. Najar M., Raicevic G., Boufker H.I., Fayyad Kazan H., De Bruyn C., Meuleman N., et al. Mesenchymal stromal cells use PGE2 to modulate activation and proliferation of lymphocyte subsets: Combined comparison of adipose tissue, Wharton's jelly and bone marrow sources. Cell Immunol 2010, 264:171-179.
11. Locke M., Windsor J., Dunbar P.R. Human adipose-derived stem cells: isolation, characterization and applications in surgery. ANZ J Surg 2009, 79:235-244.
12. Rada T., Reis R.L., Gomes M.E. Distinct stem cells subpopulations isolated from human adipose tissue exhibit different chondrogenic and osteogenic differentiation potential. Stem Cell Rev 2011, 7:64-76.
13. Oedayrajsingh-Varma M.J., van Ham S.M., Knippenberg M., Helder M.N., Klein-Nulend J., Schouten T.E., et al. Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy 2006, 8:166-177.
14. Bunnell B.A., Flaat M., Gagliardi C., Patel B., Ripoll C. Adipose-derived stem cells: isolation, expansion and differentiation. Methods 2008, 45:115-120.
15. Varma M.J.O., Breuls R.G.M., Schouten T.E., Jurgens W.J.F.M., Bontkes H.J., Schuurhuis G.J., et al. Phenotypical and functional characterization of freshly isolated adipose tissue-derived stem cells. Stem Cells Dev 2007, 16:91-104.
16. Doi K., Tanaka S., Iida H., Eto H., Kato H., Aoi N., et al. Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis. J Tissue Eng Regen Med 2013, 7:864-870.
17. Mitchell J.B., McIntosh K., Zvonic S., Garrett S., Floyd Z.E., Kloster A., et al. Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells 2006, 24:376-385.
18. Zimmerlin L., Donnenberg V.S., Pfeifer M.E., Meyer E.M., Péault B., Rubin J.P., et al. Stromal vascular progenitors in adult human adipose tissue. Cytometry A 2010, 77:22-30.
19. Araña M., Mazo M., Aranda P., Pelacho B., Prosper F. Adipose tissue-derived mesenchymal stem cells: isolation, expansion, and characterization. Methods Mol Biol 2013, 1036:47-61.
20. Baptista L.S., do Amaral R.J.F.C., Carias R.B.V., Aniceto M., Claudio-da-Silva C., Borojevic R. An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples. Cytotherapy 2009, 11:706-715.
21. Sengenès C., Lolmède K., Zakaroff-Girard A., Busse R., Bouloumié A. Preadipocytes in the human subcutaneous adipose tissue display distinct features from the adult mesenchymal and hematopoietic stem cells. J Cell Physiol 2005, 205:114-122.
22. Rada T., Reis R.L., Gomes M.E. Novel method for the isolation of adipose stem cells (ASCs). J Tissue Eng Regen Med 2009, 3:158-159.
23. Suga H., Matsumoto D., Eto H., Inoue K., Aoi N., Kato H., et al. Functional implications of CD34 expression in human adipose-derived stem/progenitor cells. Stem Cells Dev 2009, 18:1201-1210.
24. Estes B.T., Wu A.W., Storms R.W., Guilak F. Extended passaging, but not aldehyde dehydrogenase activity, increases the chondrogenic potential of human adipose-derived adult stem cells. J Cell Physiol 2006, 209:987-995.
25. Puissant B., Barreau C., Bourin P., Clavel C., Corre J., Bousquet C., et al. Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells. Br J Haematol 2005, 129:118-129.
26. Yi T., Song S.U. Immunomodulatory properties of mesenchymal stem cells and their therapeutic applications. Arch Pharm Res 2012, 35:213-221.
27. De Kock J., Najar M., Bolleyn J., Al Battah F., Rodrigues R.M., Buyl K., et al. Mesoderm-derived stem cells: the link between the transcriptome and their differentiation potential. Stem Cells Dev 2012, 21:3309-3323.
28. McIntosh K., Zvonic S., Garrett S., Mitchell J.B., Floyd Z.E., Hammill L., et al. The immunogenicity of human adipose-derived cells: temporal changes in vitro. Stem Cells 2006, 24:1246-1253.
29. Al Battah F., De Kock J., Ramboer E., Heymans A., Vanhaecke T., Rogiers V., et al. Evaluation of the multipotent character of human adipose tissue-derived stem cells isolated by Ficoll gradient centrifugation and red blood cell lysis treatment. Toxicol In Vitro 2011, 25:1224-1230.
30. Najar M., Raicevic G., Fayyad-Kazan H., Kazan H.F., De Bruyn C., Bron D., et al. Immune-related antigens, surface molecules and regulatory factors in human-derived mesenchymal stromal cells: the expression and impact of inflammatory priming. Stem Cell Rev 2012, 8:1188-1198.
31. Faustini M., Bucco M., Chlapanidas T., Lucconi G., Marazzi M., Tosca M.C., et al. Nonexpanded mesenchymal stem cells for regenerative medicine: yield in stromal vascular fraction from adipose tissues. Tissue Eng Part C Methods 2010, 16:1515-1521.
32. Fernández Vallone V.B., Romaniuk M.A., Choi H., Labovsky V., Otaegui J., Chasseing N.A. Mesenchymal stem cells and their use in therapy: what has been achieved?. Differentiation 2013, 85:1-10.
33. Horn P., Bork S., Diehlmann A., Walenda T., Eckstein V., Ho A.D., et al. Isolation of human mesenchymal stromal cells is more efficient by red blood cell lysis. Cytotherapy 2008, 10:676-685.