Optimization of a peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the detection of bacteria and disclosure of a formamide effect

Journal of Biotechnology

Volumn 187, Issue , 2014, Pages 16-24

  Santos, Rita S ^a   Guimarães, Nuno ^a   Madureira, Pedro ^a,b   Azevedo, Nuno F ^a  

^a UNIVERSITY OF PORTO   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

Author keywords

Bacteria; Formamide; Peptidoglycan; PNA EUB338; PNA FISH

Indexed keywords

AMIDES; FISH; FLUORESCENCE; FLUORESCENCE MICROSCOPY; MICROBIOLOGY; NUCLEIC ACIDS; PEPTIDES; PROBES; TRANSMISSION ELECTRON MICROSCOPY;

FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE INTENSITIES; FORMAMIDES; PEPTIDOGLYCANS; PNA EUB338; PNA-FISH; RESPONSE SURFACE METHODOLOGY; WELL-ESTABLISHED TECHNIQUES; PEPTIDE NUCLEIC ACID;

BACTERIA;

FORMAMIDE; PEPTIDE NUCLEIC ACID; PEPTIDOGLYCAN; FORMAMIDE DERIVATIVE;

ARTICLE; BACTERIAL MEMBRANE; BACTERIAL STRAIN; BACTERIUM; BACTERIUM DETECTION; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GRAM STAINING; HYBRIDIZATION; MICROORGANISM; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RESPONSE SURFACE METHOD; TEMPERATURE; TRANSMISSION ELECTRON MICROSCOPY; BACTERIUM IDENTIFICATION; CHEMISTRY; CLASSIFICATION; METABOLISM; PROCEDURES; BACILLUS CEREUS; CONCENTRATION (PARAMETERS); ESCHERICHIA COLI; LISTERIA INNOCUA; LISTERIA MONOCYTOGENES; METHANOBACTERIUM FORMICICUM; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS; SACCHAROMYCES CEREVISIAE; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

BACTERIA; BACTERIAL TYPING TECHNIQUES; FORMAMIDES; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, ELECTRON, TRANSMISSION; PEPTIDE NUCLEIC ACIDS;

EID: 84905256076     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.06.023     Document Type: Article

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