Directed evolution of an exoglucanase facilitated by a co-expressed β-glucosidase and construction of a whole engineered cellulase system in Escherichia coli

Biotechnology Letters

Volumn 36, Issue 9, 2014, Pages 1801-1807

  Liu, Min ^a   Xie, Wenping ^a   Xu, Haoming ^a   Gu, Jiali ^a   Lv, Xiaomei ^a   Yu, Hongwei ^a   Ye, Lidan ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Cellulase; Co expression; Directed evolution; Enzyme activity; Exoglucanase; High throughput screening; Glucosidase

Indexed keywords

ENZYME ACTIVITY; ESCHERICHIA COLI; GLUCOSE;

CELLULASE; CO-EXPRESSION; DIRECTED EVOLUTION; EXOGLUCANASE; GLUCOSIDASE; HIGH-THROUGHPUT SCREENING;

GENE EXPRESSION;

ESCHERICHIA COLI;

CELLULASE; GLUCOSE;

BACTERIUM TRANSFORMATION; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; METABOLIC ENGINEERING; METABOLISM;

CELLULASE; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENETIC VECTORS; GLUCOSE; METABOLIC ENGINEERING; TRANSFORMATION, BACTERIAL;

EID: 84905239336     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-014-1541-2     Document Type: Article

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