Functional domain analysis of the cell division inhibitor EzrA

PLoS ONE

Volumn 9, Issue 7, 2014, Pages

  Land, Adrian D ^a   Luo, Qingwei ^a   Levin, Petra Anne ^a  

^a WASHINGTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC PROTEIN; EZRA PROTEIN; FTSZ PROTEIN; MEMBRANE PROTEIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CYTOSKELETON PROTEIN; EZRA PROTEIN, BACILLUS SUBTILIS; FTSZ PROTEIN, BACTERIA; PROTEIN BINDING;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL DIVISION; CONTROLLED STUDY; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; CHEMISTRY; METABOLISM; NUCLEOTIDE SEQUENCE; PHYSIOLOGY;

BACTERIAL PROTEINS; CELL DIVISION; CONSERVED SEQUENCE; CYTOSKELETAL PROTEINS; PROTEIN BINDING;

EID: 84904909319     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0102616     Document Type: Article

References (27)

1. Adams DW, Errington J (2009) Bacterial cell division: assembly, maintenance and disassembly of the Z ring. Nat Rev Microbiol 7: 642-653. doi:10.1038/nrmicro2198.
2. Harry E, Monahan L, Thompson L (2006) Bacterial cell division: the mechanism and its precison. Int Rev Cytol 253: 27-94. doi:10.1016/S0074-7696(06) 53002-5. (Pubitemid 46107114)
3. Levin PA, Kurtser IG, Grossman AD (1999) Identification and characterization of a negative regulator of FtsZ ring formation in Bacillus subtilis. Proc Natl Acad Sci USA 96: 9642-9647. (Pubitemid 29398785)
4. Haeusser DP, Schwartz RL, Smith AM, Oates ME, Levin PA (2004) EzrA prevents aberrant cell division by modulating assembly of the cytoskeletal protein FtsZ. Mol Microbiol 52: 801-814. doi:10.1111/j.1365-2958.2004.04016.x. (Pubitemid 38621749)
5. Gamba P, Veening J-W, Saunders NJ, Hamoen LW, Daniel RA (2009) Twostep assembly dynamics of the Bacillus subtilis divisome. J Bacteriol 191: 4186-4194. doi:10.1128/JB.01758-08.
6. Levin PA, Schwartz RL, Grossman AD (2001) Polymer stability plays an important role in the positional regulation of FtsZ. J Bacteriol 183: 5449-5452. (Pubitemid 32802621)
7. Haeusser DP, Garza AC, Buscher AZ, Levin PA (2007) The division inhibitor EzrA contains a seven-residue patch required for maintaining the dynamic nature of the medial FtsZ ring. J Bacteriol 189: 9001-9010. doi:10.1128/JB.01172-07.
8. Claessen D, Emmins R, Hamoen LW, Daniel RA, Errington J, et al. (2008) Control of the cell elongation-division cycle by shuttling of PBP1 protein in Bacillus subtilis. Mol Microbiol 68: 1029-1046. doi:10.1111/j.1365-2958.2008. 06210.x. (Pubitemid 351581066)
9. Hamoen LW, Meile J-C, de Jong W, Noirot P, Errington J (2006) SepF, a novel FtsZ-interacting protein required for a late step in cell division. Mol Microbiol 59: 989-999. doi:10.1111/j.1365-2958.2005.04987.x.
10. Gueiros-Filho FJ, Losick R (2002) A widely conserved bacterial cell division protein that promotes assembly of the tubulin-like protein FtsZ. Genes Dev 16: 2544-2556. doi:10.1101/gad.1014102.
11. Kawai Y, Ogasawara N (2006) Bacillus subtilis EzrA and FtsL synergistically regulate FtsZ ring dynamics during cell division. Microbiology (Reading, Engl) 152: 1129-1141. doi:10.1099/mic.0.28497-0.
12. Jorge AM, Hoiczyk E, Gomes JP, Pinho MG (2011) EzrA contributes to the regulation of cell size in Staphylococcus aureus. PLoS ONE 6: e27542. doi:10.1371/journal.pone.0027542.
13. Steele VR, Bottomley AL, Garcia-Lara J, Kasturiarachchi J, Foster SJ (2011) Multiple essential roles for EzrA in cell division of Staphylococcus aureus. Mol Microbiol 80: 542-555. doi:10.1111/j.1365-2958.2011.07591.x.
14. Considine KM, Sleator RD, Kelly AL, Fitzgerald GF, Hill C (2011) Novel listerial genetic loci conferring enhanced barotolerance in Escherichia coli. J Appl Microbiol 110: 618-630. doi:10.1111/j.1365-2672.2010.04924.x.
15. Ishikawa S, Kawai Y, Hiramatsu K, Kuwano M, Ogasawara N (2006) A new FtsZ-interacting protein, YlmF, complements the activity of FtsA during progression of cell division in Bacillus subtilis. Mol Microbiol 60: 1364-1380. doi:10.1111/j.1365-2958.2006.05184.x. (Pubitemid 43801431)
16. Weiss DS, Chen JC, Ghigo JM, Boyd D, Beckwith J (1999) Localization of FtsI (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL. J Bacteriol 181: 508-520. (Pubitemid 29045142)
17. Mo AH, Burkholder WF (2010) YneA, an SOS-induced inhibitor of cell division in Bacillus subtilis, is regulated posttranslationally and requires the transmembrane region for activity. J Bacteriol 192: 3159-3173. doi:10.1128/JB.00027-10.
18. McDonnell AV, Jiang T, Keating AE, Berger B (2006) Paircoil2: improved prediction of coiled coils from sequence. Bioinformatics 22: 356-358. doi:10.1093/bioinformatics/bti797. (Pubitemid 43205384)
19. Son SH, Lee HH (2013) The N-terminal domain of EzrA binds to the C terminus of FtsZ to inhibit Staphylococcus aureus FtsZ polymerization. Biochemical and Biophysical Research Communications 433: 108-114. doi:10.1016/j.bbrc.2013.02.055.
20. Ireton K, Rudner DZ, Siranosian KJ, Grossman AD (1993) Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor. Genes Dev 7: 283-294. (Pubitemid 23084404)
21. Chivers PT, Sauer RT (1999) NikR is a ribbon-helix-helix DNA-binding protein. Protein Sci 8: 2494-2500. doi:10.1110/ps.8.11.2494. (Pubitemid 29536417)
22. LeDeaux JR, Grossman AD (1995) Isolation and characterization of kinC, a gene that encodes a sensor kinase homologous to the sporulation sensor kinases KinA and KinB in Bacillus subtilis. J Bacteriol 177: 166-175.
23. Weart RB, Nakano S, Lane BE, Zuber P, Levin PA (2005) The ClpX chaperone modulates assembly of the tubulin-like protein FtsZ. Mol Microbiol 57: 238-249. doi:10.1111/j.1365-2958.2005.04673.x. (Pubitemid 40896611)
24. Weart RB, Lee AH, Chien A-C, Haeusser DP, Hill NS, et al. (2007) A metabolic sensor governing cell size in bacteria. Cell 130: 335-347. doi:10.1016/j.cell.2007.05.043. (Pubitemid 47081134)
25. Haeusser DP, Lee AH, Weart RB, Levin PA (2009) ClpX inhibits FtsZ assembly in a manner that does not require its ATP hydrolysis-dependent chaperone activity. J Bacteriol 191: 1986-1991. doi:10.1128/JB.01606-07.
26. Levin PA (2002) 6 Light microscopy techniques for bacterial cell biology. Methods in Microbiology. Methods in Microbiology. Elsevier, Vol. 31. pp. 115-132. doi:10.1016/S0580-9517(02)31007-9.
27. Levin PA, Losick R (1996) Transcription factor Spo0A switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis. Genes Dev 10: 478-488.