Development and evaluation of a duplex real-time RT-PCR for detection and differentiation of virulent and variant strains of porcine epidemic diarrhea viruses from the United States

Journal of Virological Methods

Volumn 207, Issue , 2014, Pages 154-157

  Wang, Leyi ^a   Zhang, Yan ^a   Byrum, Beverly ^a  

^a Animal Disease Diagnostic Laboratory   (United States)

Author keywords

Differentiation; Duplex real time RT PCR; Porcine epidemic diarrhea virus (PEDV); Variant PEDV; Virulent PEDV

Indexed keywords

ARTICLE; CONSENSUS SEQUENCE; CONTROLLED STUDY; COPY NUMBER VARIATION; CORONAVIRUS; DIARRHEA; ENCEPHALOMYOCARDITIS VIRUS; EPIDEMIC; LIMIT OF DETECTION; MORTALITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PIGLET; PORCINE EPIDEMIC DIARRHEA VIRUS; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PRIORITY JOURNAL; PROTEIN DOMAIN; PSEUDORABIES HERPETOVIRUS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; STRAIN DIFFERENCE; SWINE INFLUENZA VIRUS; UNITED STATES; VIRUS DETECTION; VIRUS SPIKE; VIRUS STRAIN; VIRUS VIRULENCE; ANIMAL; CLASSIFICATION; CORONAVIRUS INFECTIONS; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR DIAGNOSIS; MULTIPLEX POLYMERASE CHAIN REACTION; PIG; PROCEDURES; SWINE DISEASES; VALIDATION STUDY; VETERINARY; VETERINARY MEDICINE; VIROLOGY;

ANIMALS; CORONAVIRUS INFECTIONS; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; PORCINE EPIDEMIC DIARRHEA VIRUS; REAL-TIME POLYMERASE CHAIN REACTION; SWINE; SWINE DISEASES; UNITED STATES; VETERINARY MEDICINE; VIROLOGY;

EID: 84904898407     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.07.005     Document Type: Article

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