Imaging flow cytometry documents incomplete resistance of human sickle F-cells to ex vivo hypoxia-induced sickling

Blood

Volumn 124, Issue 4, 2014, Pages 658-660

  Fertrin, Kleber Yotsumoto ^a   Van Beers, Eduard J ^a   Samsel, Leigh ^a   Mendelsohn, Laurel G ^a   Saiyed, Rehan ^a   Nichols, James S ^a   Hepp, David A ^a   Brantner, Christine A ^a   Daniels, Mathew P ^a   McCoy, J Philip ^a   Kato, Gregory J ^b  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HEMOGLOBIN F; HEMOGLOBIN S;

ADULT; CELL ASSAY; CELL HYPOXIA; CELL STRAIN F; CLINICAL ARTICLE; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; DISEASE RESISTANCE; ERYTHROCYTE COUNT; ERYTHROCYTE KINETICS; EX VIVO STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HYPOXIC CELL; LETTER; PRIORITY JOURNAL; PROTEIN EXPRESSION; SICKLE CELL; SICKLE CELL ANEMIA; SICKLE IMAGING FLOW CYTOMETRY ASSAY;

ANEMIA, SICKLE CELL; FETAL HEMOGLOBIN; HUMANS;

EID: 84904861589     PISSN: 00064971     EISSN: 15280020     Source Type: Journal    
DOI: 10.1182/blood-2014-03-559054     Document Type: Letter

References (4)

1. Steinberg MH, Chui DHK, Dover GJ, Sebastiani P, Alsultan A. Fetal hemoglobin in sickle cell anemia: a glass half full? Blood. 2014;123(4): 481-485.
2. van Beers EJ, Samsel L, Mendelsohn LG, et al. Imaging flow cytometry for fully automated quantification of percentage of sickled cells in sickle cell anemia. Am J Hematol. 2014;89(6):598-603.
3. Steinberg MH, Lu ZH, Barton FB, Terrin ML, Charache S, Dover GJ; Multicenter Study of Hydroxyurea. Fetal hemoglobin in sickle cell anemia: determinants of response to hydroxyurea. Blood. 1997;89(3):1078-1088. (Pubitemid 27121303)
4. Segel GB, Simon W, Lichtman MA. Should we still be focused on red cell hemoglobin F as the principal explanation for the salutary effect of hydroxyurea in sickle cell disease? Pediatr Blood Cancer. 2011;57(1):8-9.