Identification and affinity-quantification of ß-amyloid and α-synuclein polypeptides using on-line SAW-biosensor-mass spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 25, Issue 8, 2014, Pages 1472-1481

  Slamnoiu, Stefan ^a   Vlad, Camelia ^a,d   Stumbaum, Mihaela ^a,d   Moise, Adrian ^a   Lindner, Kathrin ^a   Engel, Nicole ^a,e   Vilanova, Mar ^c   Diaz, Mireia ^c   Karreman, Christiaan ^a   Leist, Marcel ^a   Ciossek, Thomas ^b   Hengerer, Bastian ^b   Vilaseca, Marta ^c   Przybylski, Michael ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

^c INSTITUTE FOR RESEARCH IN BIOMEDICINE   (Spain)

^d SAW Instruments GmbH   (Germany)

^e VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Affinity quantification; Identification; Ion trap MS; Microfluidic affinity MS interface; Mouse brain homogenate; On line bioaffinity mass spectrometry; amyloid; Synucleins; Surface acoustic wave (SAW) biosensor; Waters Synapt QTOF MS; Synucleins

Indexed keywords

ACOUSTIC SURFACE WAVE DEVICES; ACOUSTIC WAVES; ACOUSTICS; BIOLOGICAL MATERIALS; DESALINATION; GLYCOPROTEINS; IDENTIFICATION (CONTROL SYSTEMS); LIGANDS; MASS SPECTROMETERS; MASS SPECTROMETRY; MICROFLUIDICS; NEURODEGENERATIVE DISEASES; POLYPEPTIDES; SAWS;

AFFINITY QUANTIFICATION; AFFINITY-MS; BIOMOLECULAR INTERACTIONS; DETECTION AND QUANTIFICATIONS; MASS SPECTROMETRIC IDENTIFICATION; MOUSE BRAIN; STRUCTURAL CHARACTERIZATION; SURFACE ACOUSTIC WAVES;

BIOSENSORS;

ALPHA SYNUCLEIN; AMYLOID BETA PROTEIN; POLYPEPTIDE; WATER; EPITOPE; MONOCLONAL ANTIBODY; MUTANT PROTEIN; RECOMBINANT PROTEIN; SNCA PROTEIN, HUMAN;

ARTERIAL FILTER; ARTICLE; BIOSENSOR; BRAIN HOMOGENATE; CHEMICAL STRUCTURE; COMPUTER PROGRAM; MASS SPECTROMETRY; PATHOPHYSIOLOGY; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; ANTIBODY AFFINITY; BRAIN; CHEMISTRY; CYCLOTRON; FEASIBILITY STUDY; FOURIER ANALYSIS; GENETIC PROCEDURES; GENETICS; HUMAN; METABOLISM; MICROFLUIDIC ANALYSIS; MOLECULAR WEIGHT; NERVE CELL; RADIATION RESPONSE; SOUND; TRANSGENIC MOUSE;

ALPHA-SYNUCLEIN; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMYLOID BETA-PEPTIDES; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; BIOSENSING TECHNIQUES; BRAIN; CYCLOTRONS; EPITOPES; FEASIBILITY STUDIES; FOURIER ANALYSIS; HUMANS; MASS SPECTROMETRY; MICE, TRANSGENIC; MICROFLUIDIC ANALYTICAL TECHNIQUES; MOLECULAR WEIGHT; MUTANT PROTEINS; NEURONS; RECOMBINANT PROTEINS; SOUND;

EID: 84904858139     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-014-0904-1     Document Type: Article

References (28)

1. Vo-Dinh, T., Cullum, B.: Biosensors and biochips: advances in biological and medical diagnostics. Fresenius J. Anal. Chem. 366, 540-551 (2000)
2. George, A.J.: Use of biosensors to measure the kinetics of antibody-antigen interactions. Methods Mol. Med. 40, 363-72 (2000)
3. Xu, Y., Yang, X., Wang, E.: Review: aptamers in microfluidic chips. Anal. Chim. Acta. 683(1), 12-20 (2010)
4. Boozer, C., Kim, G., Cong, S., Guan, H., Londergan, T.: Looking towards label-free biomolecular interaction analysis in a high-throughput format: a review of new surface plasmon resonance technologies. Curr. Opin. Biotechnol. 17(4), 400-405 (2006)
5. Kausaite, A., Ramanaviciene, A., Mostovojus, V., Ramanavicius, A.: Surface plasmon resonance and its application to biomedical research. Medicina (Kaunas) 43(5), 355-365 (2007)
6. Tuantranont, A., Wisitsora-at, A., Sritongkham, P., Jaruwongrungsee, K.: A review of monolithic multichannel quartz crystal microbalance: a review. Anal. Chim. Acta. 687(2), 114-128 (2011)
7. Cooper, M.A., Singleton, V.T.: A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions. J. Mol. Recognit. 20(3), 154-184 (2007)
8. Marx, K.A.: The Quartz crystal microbalance and the electrochemical QCM: applications to studies of thin polymer films, electron transfer systems, biological macromolecules, biosensors, and cells piezoelectric sensors. In: Steinem, C., Janshoff, A. (eds.) vol. 5, pp. 371-424. Springer, Berlin Heidelberg (2007)
9. Li, J., Schantz, A., Schwegler, M., Shankar, G.: Detection of low-affinity anti-drug antibodies and improved drug tolerance in immunogenicity testing by Octet biolayer interferometry. J. Pharm. Biomed. Anal. 54(2), 286-294 (2011)
10. Concepcion, J., Witte, K., Wartchow, C., Choo, S., Yao, D., Persson, H., Wei, J., Li, P., Heidecker, B., Ma, W., Varma, R., Zhao, L.-S., Perillat, D., Carricato, G., Recknor, M., Du, K., Ho, H., Ellis, T., Gamez, J., Howes, M., Phi-Wilson, J., Lockard, S., Zuk, R., Tan, H.: Label-free detection of biomolecular interactions using biolayer interferometry for kinetic characterization. Comb. Chem. High Throughput Screen. 12(8), 791-800 (2009)
11. Gronewold, T.M.A.: Surface acoustic wave sensors in the bioanalytical field: recent trends and challenges. Anal. Chim. Acta. 603(2), 119-128 (2007)
12. Länge, K., Rapp, B., Rapp, M.: Surface acoustic wave biosensors: a review. Anal. Bioanal. Chem. 391(5), 1509-1519 (2008)
13. Dragusanu, M., Petre, B.A., Slamnoiu, S., Vlad, C., Tu, T., Przybylski, M.: On-line bioaffinity-electrospray mass spectrometry for simultaneous detection, identification, and quantification of protein-ligand interactions. J. Am. Soc. Mass Spectrom. 21(10), 1643-1648 (2010)
14. Marshall, A.G., Hendrickson, C.L., Jackson, G.S.: Fourier transform ion cyclotron resonance mass spectrometry: a primer. Mass Spectrom. Rev. 17(1), 1-35 (1998)
15. Marshall, A.G., Hendrickson, C.L., Emmett, M.R., Rodgers, R.P., Blakney, G.T., Nilsson, C.L.: Fourier transform ion cyclotron resonance: state of the art. Eur. J. Mass Spectrom. 13(1), 57-59 (2007)
16. Vlad, C., Lindner, K., Karreman, C., Schildknecht, S., Leist, M., Tomczyk, N., Rontree, J., Langridge, J., Danzer, K., Ciossek, T., Petre, A., Gross, M.L., Hengerer, B., Przybylski, M.: Autoproteolytic fragments are intermediates in the oligomerization/aggregation of the Parkinson's disease protein alpha-synuclein as revealed by ion mobility mass spectrometry. Chem. Biochem. 12(18), 2740-2744 (2011)
17. McLaurin, J., Cecal, R., Kierstead, M.E., Tian, X., Phinney, A.L., Manea, M., French, J.E., Lambermon, M.H., Darabie, A.A., Brown, M.E., Janus, C., Chishti, M.A., Horne, P., Westaway, D., Fraser, P.E., Mount, H.T., Przybylski, M., St. George-Hyslop, P.: Therapeutically effective antibodies against amyloid-beta peptide target amyloid-beta residues 4-10 and inhibit cytotoxicity and fibrillogenesis. Nat. Med. 8(11), 1263-1269 (2002)
18. Lindner, K.: Oligomerization-aggregation and fragmentation of synuclein polypeptides revealed by mass spectrometry. Dissertation, University of Konstanz (2014)
19. Juszczyk, P., Paraschiv, G., Szymanska, A., Kolodziejczyk, A.S., Rodziewicz-Motowidlo, S., Grzonka, Z., Przybylski, M.: Binding epitopes and interaction structure of the neuroprotective protease inhibitor cystatin C with beta-amyloid revealed by proteolytic excision mass spectrometry and molecular docking simulation. J. Med. Chem. 52(8), 2420-2428 (2009)
20. Cozma, A.: Development of a software for performing automated on-line bioaffinity-mass spectrometry. Diploma Thesis, University of Konstanz (2012)
21. Susnea, I., Bunk, S., Wendel, A., Hermann, C., Przybylski, M.: Biomarker candidates of Chlamydophila pneumoniae proteins and protein fragments identified by affinity-proteomics using FTICR-MS and LC-MS/MS. J. Am. Soc. Mass Spectrom. 22(4), 784-788 (2011)
22. Freichel, C., Neumann, M., Ballard, T., Muller, V., Woolley, M., Ozmen, L., Borroni, E., Kretzschmar, H.A., Haass, C., Spooren, W., Kahle, P.J.: Age-dependent cognitive decline and amygdala pathology in alpha-synuclein transgenic mice. Neurobiol. Aging 28(9), 1421-1435 (2007)
23. Maulet, Y., Cox, J.A.: Structural changes in melittin and calmodulin upon complex formation and their modulation by calcium. Biochemistry 22, 5680-5686 (1983)
24. Wang, Y.J., Zhou, H.D., Zhou, X.F.: Clearance of amyloid-beta in Alzheimer's disease: progress, problems, and perspectives. Drug Discov. Today 11(19-20), 931-938 (2006)
25. Dodel, R., Bacher, M., Przybylski, M., Stefanescu, R., Manea, M.: Diagnosis of Alzheimer's disease and other neurodementing disorders. Patent International Application no.: PCT/IB2008/000456, Pub. no.: WO/2008/084402, European Patent Office (2008)
26. Crews, L., Tsigelny, I., Hashimoto, M., Masliah, E.: Role of synucleins in Alzheimer's disease. Neurotox. Res. 16(3), 306-317 (2009)
27. Vlad, C.: Oligomerization, degradation, and aggregation reactions and products of synuclein polypeptides related to Parkinson's Disease. Dissertation, University of Konstanz (2011)
28. Tau (τ 5) monoclonal antibody, purified, catalog number SIG-39413. Available at: https://store.crpinc.com/datasheet.aspx?Catalogno=SIG-39413. Accessed 23 May 2013