Modification of commercially available image analysis software for semi-automated qualitative analysis of axon regeneration and myelination in the rat sciatic nerve

Journal of Neuroscience Methods

Volumn 233, Issue , 2014, Pages 45-49

  Isaacs, Jonathan ^a   Mallu, Satya ^a   Batchelor, Matthew ^b  

^a VIRGINIA COMMONWEALTH UNIVERSITY MEDICAL CENTER   (United States)

^b Alces Imaging and Automation LLC 2906 Blue Wind Court   (United States)

Author keywords

G Ratio; Manual axon measurement; Sciatic nerve; Semi automated axon analysis

Indexed keywords

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; HISTOLOGY; IMAGE ANALYSIS; IMAGING SOFTWARE; MYELIN SHEATH; MYELINATION; NERVE FIBER; NERVE FIBER REGENERATION; NONHUMAN; PRIORITY JOURNAL; RAT; SCIATIC NERVE; ANIMAL; AUTOMATED PATTERN RECOGNITION; CELL COUNT; CELL SIZE; COMPUTER PROGRAM; DISEASE MODEL; FEMALE; IMAGE PROCESSING; INJURIES; MICROPHOTOGRAPHY; NERVE REGENERATION; PATHOLOGY; PATHOPHYSIOLOGY; PHYSIOLOGY; PROCEDURES; SPRAGUE DAWLEY RAT; TIME;

ANIMALS; AXONS; CELL COUNT; CELL SIZE; DISEASE MODELS, ANIMAL; FEMALE; IMAGE PROCESSING, COMPUTER-ASSISTED; MYELIN SHEATH; NERVE REGENERATION; PATTERN RECOGNITION, AUTOMATED; PHOTOMICROGRAPHY; RATS, SPRAGUE-DAWLEY; SCIATIC NERVE; SOFTWARE; TIME FACTORS;

EID: 84904596762     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2014.05.032     Document Type: Article

References (10)

1. Auer R.N. Automated nerve fibre size and myelin sheath measurement using microcomputer-based digital image analysis: theory, method and results. J Neurosci Methods 1994, 51:229-238.
2. da Silva A.P., Jordao C.E., Fazan V.P. Peripheral nerve morphometry: comparison between manual and semi-automated methods in the analysis of a small nerve. J Neurosci Methods 2007, 159:153-157.
3. Friede R.L., Beuche W. Combined scatter diagrams of sheath thickness and fibre calibre in human sural nerves: changes with age and neuropathy. J Neurol Neurosurg Psychiatry 1985, 48:749-756.
4. Mezin P., Tenaud C., Bosson J.L., Stoebner P. Morphometric analysis of the peripheral nerve: advantages of the semi-automated interactive method. J Neurosci Methods 1994, 51:163-169.
5. More H.L., Chen J., Gibson E., Donelan J.M., Beg M.F. A semi-automated method for identifying and measuring myelinated nerve fibers in scanning electron microscope images. J Neurosci Methods 2011, 201:149-158.
6. Romero E., Cuisenaire O., Denef J.F., Delbeke J., Macq B., Veraart C. Automatic morphometry of nerve histological sections. J Neurosci Methods 2000, 97:111-122.
7. Rushton W.A. A theory of the effects of fibre size in medullated nerve. J Physiol 1951, 115:101-122.
8. Savy C., Margules S., Solari A., Saint-Jean P., Farkas-Bargeton E. An image analysis morphometric method for the study of myelinated nerve fibers from mouse trigeminal root. Anal Quant Cytol Histol 1988, 10:307-316.
9. Vita G., Santoro M., Trombetta G., Leonardi L., Messina C. A computer-assisted automatic method for myelinated nerve fiber morphometry. Acta Neurol Scand 1992, 85:18-22.
10. Wood M.D., Kemp S.W., Weber C., Borschel G.H., Gordon T. Outcome measures of peripheral nerve regeneration. Ann Anat 2011, 193:321-333.