Hydrogen peroxide staining to visualize intracellular bacterial infections of seedling root cells

Microscopy Research and Technique

Volumn 77, Issue 8, 2014, Pages 566-573

  White, James F ^a   Torres, Mónica S ^a   Somu, Mohini P ^a   Johnson, Holly ^b   Irizarry, Ivelisse ^a   Chen, Qiang ^a   Zhang, Ning ^a   Walsh, Emily ^a   Tadych, Mariusz ^a   Bergen, Marshall ^a  

^a RUTGERS UNIVERSITY   (United States)

^b CENTRAL WASHINGTON UNIVERSITY   (United States)

Author keywords

Hydrogen peroxide staining; Intracellular bacteria; Light microscopy; Symbiosis

Indexed keywords

ANILINE; CELLS; HISTOLOGY; HYDROGEN PEROXIDE; OXIDATION; PLANT CELL CULTURE; PLANTS (BOTANY); PROTEINS; SEED; TISSUE; VISUALIZATION;

ANILINE BLUE; BACTERIAL CELLS; HYDROGEN PEROXIDE STAINING; INTRACELLULAR BACTERIA; PLANTS CELLS; ROOT CELLS; ROOT TISSUES; SEEDLING ROOTS; STAINING METHOD; SYMBIOSIS;

BACTERIA;

HYDROGEN PEROXIDE;

BURKHOLDERIA GLADIOLI; CHEMISTRY; MICROBIOLOGY; PANICUM; PLANT ROOT; PROCEDURES; SEEDLING; STAINING; ULTRASTRUCTURE;

BURKHOLDERIA GLADIOLI; HYDROGEN PEROXIDE; PANICUM; PLANT ROOTS; SEEDLING; STAINING AND LABELING;

EID: 84904437650     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.22375     Document Type: Article

References (47)

1. Amijee F, Allan EJ, Waterhouse RN, Glover LA, Paton AM. 1992. Nonpathogenic association of L-form bacteria (Pseudomonas syringae pv. phaseolicola) with bean plants (Phaseolus vulgaris L.) and its potential for biocontrol of halo blight disease. Biocontrol Sci Technol 2:203-214.
2. Arnold AE, Lutzoni F. 2007. Diversity and host range of foliar fungal endophytes: Are tropical leaves biodiversity hotspots? Ecology 88:541-549.
3. Baker GC, Smith JJ, Cowan DA. 2003. Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55:541-555.
4. Beran V, Havelkova M, Kaustova J, Dvorska L, Pavlik I. 2006. Cell wall deficient forms of mycobacteria: A review. Vet Med 51:365-389.
5. Chia TF, Aung HH, Osipov A, Goh NK, Chia LS. 2004. Carnivorous pitcher plant uses free radicals in the digestion of prey. Redox Report 9:255-261.
6. Clay K, Holah J, Rudgers JA. 2005. Herbivores cause a rapid increase in hereditary symbiosis and alter plant community composition. Proc Natl Acad Sci USA 102:12465-12470.
7. Compant S, Reiter B, Sessitsch A, Nowak J, Clement C, Ait Barka E. 2005. Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN. Appl. Environ. Microbiol. 71:1685-1693.
8. Daulagala PWHKP, Allan EJ. 2003. L-form bacteria of Pseudomonas syringae pv. phaseolicola induce chitinases and enhance resistance to Botrytis cinerea infection in Chinese cabbage. Physiol Mol Plant Pathol 62:253-263.
9. Galek H, Osswald WF, Elstner EF. 1990. Oxidative protein modification as predigestive mechanism of the carnivorous plant Dionaea muscipula: an hypothesis based on in vitro experiments. Free Radic. Biol. Med. 9:427-434.
10. Godlewski M, Adamczyk B. 2007. The ability of plants to secrete proteases by roots. Plant Physiol Biochem 45:657-664.
11. Hill PW, Quilliam RS, DeLuca TH, Farrar J, Farrell M, Roberts P, Newsham KK, Hopkins DW, Bardgett RD, Jones DL. 2011. Acquisition and assimilation of nitrogen as peptide-bound and D-enantiomers of amino acids by wheat. PLoS One 6:e19220. doi:10.1371/journal.pone.0019220.
12. Hood ME, Shew HD. 1997. Initial cellular interactions between Thielaviopsis basicola and tobacco root hairs. Phytopathology 87:228-235.
13. Hurek T, Reinhold-Hurek B, Kellenberger E, Van Montagu M. 1994. Root colonization and systemic spreading of Azoarcus sp. strain BH72 in grasses. J Bacteriol 176:1913-1923.
14. James EK, Reis VM, Olivares FL, Baldani JI, Dobereiner J. 1994. Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus. J Exp Bot 45:757-766.
15. Jamtgard S, Nasholm T, Huss-Danell K. 2008. Characteristics of amino acid uptake in barley. Plant Soil 302:221-231.
16. Johnston-Monje D, Raizada MN. 2011. Conservation and diversity of seed associated endophytes in Zea across boundaries of evolution, ethnography and ecology. PLoS One 6:e20396.
17. Kamarova NY, Thor K, Gubler A, Meier S, Dietrich D, Weichert A, Suter Grotemeyer M, Tegeder M, Rentsch D. 2008. AtPTR1 and AtPTR5 transport dipeptides in planta. Plant Physiol 148:856-869.
18. Kielland K. 1994. Amino acid absorption by arctic plants: Implications for plant nutrition and nitrogen cycling. Ecology 75:2373-2383.
19. Kloepper JW. 1993. Plant growth-promoting rhizobacteria as biological control agents. In: Metting FB, Jr, editor. Soil microbial ecology: Applications in agricultural and environmental management. New York, USA: Marcel Dekker Inc. pp. 255-274.
20. Kovtunovych G, Lar O, Kamalova S, Kordyum V, Kleiner D, Kozyrovska N. 1999. Correlation between pectate lyase activity and ability of diazotrophic Klebsiella oxytoca VN 13 to penetrate into plant tissues. Plant and Soil 215:1-6.
21. Kuldau G, Bacon CW. 2008. Clavicipitaceous endophytes: Their ability to enhance grass resistance to multiple stresses. Biol Control 46:57-71.
22. Lamb C, Dixon RA. 1997. The oxidative burst in plant disease resistance. Annu Rev Plant Physiol Mol Biol 48:251-275.
23. Magnani G, Didonet C, Cruz L, Picheth C, Pedrosa F, Souza E. 2010. Diversity of endophytic bacteria in Brazilian sugarcane. Genet Mol Res 9:250-258.
24. Matsubayashi Y, Sakagami Y. 1996. Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L. Proc Natl Acad Sci USA 93:7623-7627.
25. Mikola J. 1998. Effects of microbivore species composition and basal resource enrichment on trophic-level biomasses in an experimental microbial-based soil food web. Oecologia 117:396-403.
26. Munkres KD. 1990. Histochemical detection of superoxide radicals and hydrogen peroxide by Age-1 mutants of Neurospora. Fungal Genet Newslett 37:24-25.
27. Näsholm T, Kielland K, Ganeteg U. 2009. Uptake of organic nitrogen by plants. New Phytol 182:31-48.
28. Paungfoo-Lonhienne C, Lonhienne TGA, Rentsch D, Robinson N, Christie M, Webb RI, Gamage HK, Carroll BJ, Schenk PM, Schmidt S. 2008. Plants can use protein as a nitrogen source without assistance from other organisms. Proc Natl Acad Sci USA 105:4524-4529.
29. Paungfoo-Lonhienne C, Rentsch D, Robatzrk S, Webb RI, Sagulenko E, Nasholm T, Schmidt S, Lonhienne TGA. 2010. Turning the table: Plants consume microbes as a source of nutrients. PLoS One 5:e11915.
30. Paungfoo-Lonhienne C, Visser J, Lonhienne T, Schmidt S. 2012. Past, present and future of organic nutrients. Plant Soil 359:1-18.
31. Perrine-Walker FM, Prayitno J, Rolfe BG, Weinman JJ, Hocart CH. 2007. Infection process and the interaction of rice roots with rhizobia. J Exp Bot 58:3343-3350.
32. Pick E, Keisari Y. 1980. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Microbiol Methods 38:161-170.
33. Prieto P, Schilirò E, Maldonado-González MM, Valderrama R, Barrose-Albarracín JB, Mercado-Blanco J. 2011. Root hairs play a key role in endophytic colonization of olive roots by Pseudomonas spp. with biocontrol activity. Microb Ecol 62:435-445.
34. Puente ME, Bashan Y. 1994. The desert epiphyte Tillandsia recurvata harbours the nitrogen-fixing bacterium Pseudomonas stutzeri. Can J Bot 72:406-408.
35. Redman RS, Sheehan KB, Stout RG, Rodriguez RJ, Henson JM. 2002. Thermotolerance generated by plant/fungal symbiosis. Science 298:1581.
36. Reinhold-Hurek B, Hurek T. 2011. Living inside plants: Bacterial endophytes. Curr Opin Plant Biol 14:435-443.
37. Robinson JM. 2008. Reactive oxygen species in phagocytic leucocytes. Histochem Cell Biol 130:281-297.
38. Rodriguez RJ, Woodward C, Kim YO, Redman RS. 2009. Habitat-adapted symbiosis as a defense against abiotic and biotic stresses. In: White JF, Jr, Torres MS, editors. Defensive mutualism in microbial symbiosis. Boca Raton, FL, USA: CRC Press. pp. 335-346.
39. Rosenblueth M, Martínez-Romero E. 2006. Bacterial endophytes and their interactions with hosts. Mol Plant Microbe Interact 19:826-837.
40. Selosse M-A, Roy M. 2008. Green plants that feed on fungi. Trends Plant Sci 14:64-70.
41. Stone JK, Bacon CW, White JF, Jr. 2000. An overview of endophytic microbes: endophytism defined. In: Bacon CW, White JF, Jr, editors. Microbial endophytes. New York, USA: Marcel-Dekker. pp. 3-30.
42. Tedersoo L, Pellet P, Koljalg U, Selosse M. 2007. Parallel evolutionary paths to mycoheterotrophy in understorey Ericaceae and Orchidaceae: Ecological evidence for mixotrophy in Pyroleae. Oecologia 151:206-217.
43. Thomas P, Reddy KM. 2013. Microscopic elicidation of abundant endophytic bacteria colonizing the cell wall-plasma membrane peri-space in the shoot-tip tissue of banana. AoB PLANTS 5:plt011; doi:10.1093/aobpla/plt011.
44. Thomas P, Soly TA. 2009. Endophytic bacteria associated with growing shoot tips of banana (Musa sp.) cv. Grand Naine and the affinity of endophytes to the host. Microb Ecol1 58:953-964.
45. Wall S, Kunze ZM, Saboor S, Soufleri I, Seechurn P, Chiodini R, McFadden JJ. 1993. Identification of spheroplast-like agents isolated from tissues of patients with Crohn's disease and control tissues by polymerase chain reaction. J Clin Microbiol 31:1241-1245.
46. White JF, Torres MS. 2010. Is endophyte-mediated defensive mutualism oxidative stress protection? Physiol Plant 138:440-446.
47. White JF, Jr, Crawford H, Torres MS, Mattera R, Bergen M, Irisarry I. 2012. A proposed mechanism for nitrogen acquisition by grass seedlings through oxidation of symbiotic bacteria. Symbiosis 57:161-171.