Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene

Molecular Biotechnology

Volumn 56, Issue 7, 2014, Pages 621-630

  Sakaguchi, Masakiyo ^a   Watanabe, Masami ^a,b   Kinoshita, Rie ^c   Kaku, Haruki ^a,b   Ueki, Hideo ^a   Futami, Junichiro ^c   Murata, Hitoshi ^a   Inoue, Yusuke ^d   Li, Shun Ai ^a   Huang, Peng ^a   Putranto, Endy Widya ^a   Ruma, I Made Winarsa ^a   Nasu, Yasutomo ^a,b   Kumon, Hiromi ^a   Huh, Nam Ho ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b OKAYAMA UNIVERSITY HOSPITAL   (Japan)

^c OKAYAMA UNIVERSITY   (Japan)

^d GUNMA UNIVERSITY   (Japan)

Author keywords

Adenovirus; Gene expression; Gene therapy; Plasmid; Recombinant protein

Indexed keywords

GENE THERAPY; RECOMBINANT PROTEINS;

ADENOVIRUS; EXPRESSION LEVELS; EXPRESSION SYSTEM; EXPRESSION VECTORS; HIGHER EFFICIENCY; PLASMID; POLYADENYLATION SIGNALS; TANDEM PROMOTERS;

GENE EXPRESSION;

COMPLEMENTARY DNA; ELONGATION FACTOR 1ALPHA; HERPESVIRUS VECTOR; PLASMID VECTOR; RECOMBINANT PROTEIN; TELOMERASE REVERSE TRANSCRIPTASE; TELOMERASE; TERT PROTEIN, HUMAN;

ANIMAL CELL; ARTICLE; CAG REPEAT; CELL TYPE; CONTROLLED STUDY; CYTOMEGALOVIRUS; EMBRYO; EXPRESSION VECTOR; FEMALE; GENE EXPRESSION SYSTEM; GENE INSERTION; HEK293 CELL LINE; HELA CELL LINE; HEPG2 CELL LINE; HUMAN; HUMAN CELL; MALE; MAMMAL CELL; MCF 7 CELL LINE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYADENYLATION; PROMOTER REGION; SIMIAN VIRUS 40; TANDEM REPEAT; TRANSGENE; ANIMAL; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS;

ADENOVIRIDAE; MAMMALIA;

ANIMALS; GENE EXPRESSION REGULATION; GENETIC VECTORS; HUMANS; PROMOTER REGIONS, GENETIC; TELOMERASE; TRANSGENES;

EID: 84904347159     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-014-9738-0     Document Type: Article

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