Development of a new immunoassay for the detection of ethyl glucuronide (EtG) in meconium: Validation with authentic specimens analyzed using LC-MS/MS. Preliminary results

Clinical Chemistry and Laboratory Medicine

Volumn 52, Issue 8, 2014, Pages 1179-1185

  Pichini, Simona ^a   Morini, Luca ^b   Pacifici, Roberta ^a   Tuyay, James ^c   Rodrigues, Warren ^c   Solimini, Renata ^a   Garcia Algar, Oscar ^d   Ramis, Juan ^d   Moore, Christine ^c  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b Department of Public Health Experimental and Forensic Medicine ^*  (Italy)

^c Immunalysis Corporation   (United States)

^d HOSPITAL DEL MAR   (Spain)

Author keywords

ethylglucuronide; immunoassay; meconium; prenatal exposure to ethanol

Indexed keywords

ETHYL GLUCURONIDE; BIOLOGICAL MARKER; GLUCURONIC ACID;

ARTICLE; CALIBRATION; CONCENTRATION (PARAMETERS); CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOASSAY; LIQUID CHROMATOGRAPHY; MECONIUM; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; CHEMISTRY; FEMALE; METABOLISM; NEWBORN; PREGNANCY; PROCEDURES; REPRODUCIBILITY;

BIOLOGICAL MARKERS; CHROMATOGRAPHY, LIQUID; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GLUCURONATES; HUMANS; INFANT, NEWBORN; MECONIUM; PREGNANCY; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

EID: 84903973604     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/cclm-2013-1087     Document Type: Article

References (14)

1. Hutson JR, Rao C, Fulga N, Aleksa K, Koren G. An improved method for rapidly quantifying fatty acid ethyl esters in meconium suitable for prenatal alcohol screening. Alcohol 2011;45:193-9.
2. Bearer CF, Jacobson JL, Jacobson SW, Barr D, Croxford J, Molteno CD, et al. Validation of a new biomarker of fetal exposure to alcohol. J Pediatr 2003;143:463-9.
3. Moore C, Jones J, Lewis D, Buchi K. Prevalence of fatty acid ethyl esters in meconium specimens. Clin Chem 2003;49:133-6.
4. Ostrea EM, Hernandez JD, Bielawski DM, Kan JM, Leonardo GM, Abela MB, et al. Fatty acid ethyl esters in meconium: are they biomarkers of fetal alcohol exposure and effect? Alcohol Clin Exp Res 2006;30:1152-9.
5. Pichini S, Pellegrini M, Gareri J, Koren J, García-Algar O, Vall O, et al. Alarming prevalence of fetal alcohol exposure in a Mediterranean city. Ther Drug Monit 2008;30:249-54.
6. Chan D, Bar-Oz B, Pellerin B, Paciorek C, Klein J, Kapur B, et al. Population baseline of meconium fatty acid ethyl esters among infants of nondrinking women in Jerusalem and Toronto. Ther Drug Monit 2003;25:271-8.
7. Gareri J, Klein J, Koren G. Drugs of abuse testing in meconium. Clin Chim Acta 2006;366:101-11.
8. Politi L, Leone F, Morini L, Polettini A. Bioanalytical procedures for determination of conjugates or fatty acid esters of ethanol as markers of ethanol consumption: a review. Anal Biochem 2007;368:1-16.
9. Morini L, Marchei E, Pellegrini M, Groppi A, Stramesi C, Vagnarelli F, et al. Liquid chromatography with tandem mass spectrometric detection for the measurement of ethyl glucuronide and ethylsulfate in meconium: new biomarkers of gestational ethanol exposure? Ther Drug Monit 2008;30: 725-32.
10. Morini L, Marchei E, Vagnarelli F, Garcia Algar O, Groppi A, Mastrobattista L, et al. Ethyl glucuronide and ethyl sulfate in meconium and hair-potential biomarkers of intrauterine exposure to ethanol. Forensic Sci Int 2010:196:74-7.
11. Burd L, Hofer R. Biomarkers for detection of prenatal alcohol exposure: a critical review of fatty acid ethyl esters in meconium. Birth Defects Res Part A Clin Mol Teratol 2008;82:487-93.
12. Pichini S, Pellegrini M, Gareri J, Koren G, Garcia-Algar O, Vall O, et al. Liquid chromatography-tandem mass spectrometry for fatty acid ethyl esters in meconium: assessment of prenatal exposure to alcohol in two European cohorts. J Pharm Biomed Anal 2008;48:927-33.
13. Spiehler V, Isenschmid D, Matthews P, Kemp P, Kupiec T. Performance of a microtiter plate ELISA for screening of postmortem blood for cocaine and metabolites. J Anal Toxicol 2003;27:587-91.
14. Morini L, Groppi A, Marchei E, Vagnarelli F, Garcia Algar O, Zuccaro P, et al. Population baseline of meconium ethyl glucuronide and ethyl sulfate concentrations in newborns of non-drinking women in 2 Mediterranean cohorts. Ther Drug Monit 2010;32:359-63.