Comparative evaluation of the Diagenode multiplex PCR assay on the BD Max system versus a routine in-house assay for detection of Bordetella pertussis

Journal of Clinical Microbiology

Volumn 52, Issue 7, 2014, Pages 2668-2670

  Kenicer, Juliet ^a   Hardie, Alison ^a   Hamilton, Fiona ^a   Gadsby, Naomi ^a   Templeton, Kate ^a  

^a ROYAL INFIRMARY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AGED; ANALYTICAL EQUIPMENT; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BORDETELLA PARAPERTUSSIS; BORDETELLA PERTUSSIS; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; EXTRACTION; FEMALE; HUMAN; INFANT; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; MIXED INFECTION; MOLECULAR DIAGNOSIS; MULTIPLEX POLYMERASE CHAIN REACTION; NASOPHARYNGEAL ASPIRATION; NONHUMAN; PERTUSSIS; POLYMERASE CHAIN REACTION SYSTEM; PREDICTIVE VALUE; PREVALENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY VIRUS; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; THROAT CULTURE; CHILD; COMPARATIVE STUDY; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; LABORATORY AUTOMATION; MICROBIOLOGY; MIDDLE AGED; NASOPHARYNX; NEWBORN; PHARYNX; PRESCHOOL CHILD; PROCEDURES; VERY ELDERLY; WHOOPING COUGH; YOUNG ADULT;

ADOLESCENT; ADULT; AGED, 80 AND OVER; AUTOMATION, LABORATORY; BORDETELLA PERTUSSIS; CHILD; CHILD, PRESCHOOL; FEMALE; HUMANS; INFANT; INFANT, NEWBORN; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; NASOPHARYNX; PHARYNX; SENSITIVITY AND SPECIFICITY; WHOOPING COUGH; YOUNG ADULT;

EID: 84903793669     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00212-14     Document Type: Article

References (18)

1. Paddock CD, Sanden GN, Cherry JD, Gal AA, Langston C, Tatti KM, Wu KH, Goldsmith CS, Greer PW, Montague JL, Eliason MT, Holman RC, Guarner J, Shieh WJ, Zaki SR. 2008. Pathology and pathogenesis of fatal Bordetella pertussis infection in infants. Clin. Infect. Dis. 47:328-338. http://dx.doi.org/10.1086/ 589753. (Pubitemid 352013513)
2. World Health Organization. 2013. Pertussis: immunization surveillance, assessment and monitoring. World Health Organization, Geneva, Switzerland. http://www.who.int/immunization-monitoring/diseases/pertussis/en/index.html.
3. Public Health England. 2013. Laboratory confirmed pertussis in England: data to end-July. Health protection report, vol 7, no 39, 27 September 2013. Public Health England, London, United Kingdom. http://www.hpa.org.uk/hpr/ archives/2013/news3913.htm.
4. Cox HC, Jacob K, Whiley DM, Bletchly C, Nimmo GR, Nissen MD, Sloots TP. 2013. Further evidence that the IS481 target is suitable for real-time PCR detection of Bordetella pertussis. Pathology 45:202-203. http://dx.doi.org/10. 1097/PAT.0b013e32835cc2d7.
5. Dalby T, Fry NK, Krogfelt KA, Jensen JS, He Q. 2013. Evaluation of PCR methods for the diagnosis of pertussis by the European surveillance network for vaccine-preventable diseases (EUVAC.NET). Eur. J. Clin. Microbiol. Infect. Dis. 32:1285-1289. http://dx.doi.org/10.1007/s10096-013-1874-0.
6. Templeton KE, Scheltinga SA, van der Zee A, Diederen BM, Kruijssen A, Goossens H, Kuijper E, Claas EC. 2003. Evaluation of real-time PCR for detection of, and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis. J. Clin. Microbiol. 41:4121-4126. http://dx.doi.org/10.1128/JCM.41.9.4121-4126.2003. (Pubitemid 37099674)
7. van der Zee A, Agterberg C, Peeters M, Schellekens J, Mooi FR. 1993. Polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of Bordetella pertussis and Bordetella parapertussis. J. Clin. Microbiol. 31:2134-2140. (Pubitemid 23210507)
8. Tatti KM, Sparks KN, Boney KO, Tondella ML. 2011. Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens. J. Clin. Microbiol. 49:4059-4066. http://dx.doi.org/10.1128/JCM. 00601-11.
9. van Kruijssen AM, Templeton KE, van der Plas RN, van Doom HR, Claas EJC, Sukhai RN, Kuijper EJ. 2007. Detection of respiratory pathogens by realtime PCR in children with clinical suspicion of pertussis. Eur. J. Paediatr. 166: 1189-1191. http://dx.doi.org/10.1007/s00431-006-0378-7.
10. Quality Control for Molecular Diagnostics. 2011. Final report. EQA programme 2011 Bordetella pertussis (BPDNA11). QAB94132. Quality Control for Molecular Diagnostics, Glasgow, Scotland.
11. Glare EM, Paton JC, Premier RR, Lawrence AJ, Nisbet IT. 1990. Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction. J. Clin. Microbiol. 28:1982-1987. (Pubitemid 20260976)
12. Parkhill J, Sebaihia M, Preston A, Murphy LD, Thomson N, Harris DE, Holden MTG, Churcher CM, Bentley SD, Mungall KL, Cerdeño-Tárraga AM, Temple L, James K, Harris B, Quail MA, Achtman M, Atkin R, Baker S, Basham D, Bason N, Cherevach I, Chillingworth T, Collins M, Cronin A, Davis P, Doggett J, Feltwell T, Goble A, Hamlin N, Hauser H, Holroyd S, Jagels K, Leather S, Moule S, Norberczak H, O'Neil S, Ormond D, Price C, Rabbinowitsch E, Rutter S, Sanders M, Saunders D, Seeger K, Sharp S, Simmonds M, Skelton J, Squares R, Squares S, Stevens K, Unwin L, Whitehead S, Barrell BG, Maskell DJ. 2003. Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nat. Genet. 35: 32-40. http://dx.doi.org/10.1038/ng1227.
13. Reischl U, Lehn N, Sanden GN, Loeffelholz MJ. 2001. Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis of detecting Bordetella holmesii. J. Clin. Microbiol. 39:1963-1966. http://dx.doi.org/10. 1128/JCM.39.5.1963-1966.2001. (Pubitemid 32417053)
14. Register KB, Sanden GN. 2006. Prevalence and sequence variant of IS481 in Bordetella bronchiseptica: implications for IS481 detection of Bordetella pertussis. J. Clin. Microbiol. 44:4577-4583. http://dx.doi.org/10.1128/JCM. 01295-06. (Pubitemid 44937101)
15. Muyldermans G, Soetens O, Antoine M, Bruisten S, Vincart B, Doucet-Populaire F, Fry NK, Olcén P, Scheftel JM, Senterre JM, van der Zee A, Riffelmann M, Piérard D, Lauwers S. 2005. External quality assessment for molecular detection of Bordetella pertussis in European laboratories. J. Clin. Microbiol. 43:30-35. http://dx.doi.org/10.1128/JCM.43.1. 30-35.2005.
16. Heim A, Ebnet G, Harste G, Pring-Akerblom P. 2003. Rapid and quantitative detection of human adenovirus DNA by real time PCR. J. Med. Virol. 70:228-239. http://dx.doi.org/10.1002/jmv.10382. (Pubitemid 36520631)
17. Templeton KE, Scheltinga SA, Graffelman AW, Van Schie JM, Crielaard JW, Sillekens P, Van Den Broek PJ, Goossens H, Beersma MF, Claas EC. 2003. Comparison and evaluation of real-time PCR, real-time nucleic acid sequence-based amplification, conventional PCR, and serology for diagnosis of Mycoplasma pneumoniae. J. Clin. Microbiol. 41:4366-4371. http://dx.doi.org/10. 1128/JCM.41.9.4366-4371.2003.
18. Templeton KE, Scheltinga SA, van den Eeden WCJFM, Graffelman AW, van den Broek PJ, Claas ECJ. 2005. Improved diagnosis of the etiology of community-acquired pneumonia with real-time polymerase chain reaction. Clin. Infect. Dis. 41:345-351. http://dx.doi.org/10.1086/431588.