Development of a method to quantify platelet adhesion and aggregation under static conditions

Cellular and Molecular Bioengineering

Volumn 7, Issue 2, 2014, Pages 285-290

  Baker Groberg, Sandra M ^a   Cianchetti, Flor A ^a   Phillips, Kevin G ^a   McCarty, Owen J T ^a  

^a Oregon Health and Science University Department of Biomedical Engineering   (United States)

Author keywords

Aggregation; Blood; Microscopy; Platelets

Indexed keywords

ADENOSINE DIPHOSPHATE; COLLAGEN; GLYCOPROTEIN IIB; GLYCOPROTEIN IIIA; PROTEIN INHIBITOR; THROMBOXANE A2;

ARTICLE; CELL INTERACTION; CELL SURFACE; CELL VOLUME; CONCENTRATION (PARAMETERS); FEEDBACK SYSTEM; MASS; PLATELET AGGREGATION ASSAY; PRIORITY JOURNAL; SINGLE CELL ANALYSIS; STANDARDIZATION; THROMBOCYTE ACTIVATION; THROMBOCYTE ADHESION; THROMBOCYTE AGGREGATION; THROMBOCYTE COUNT; THROMBOCYTE FUNCTION;

EID: 84903787509     PISSN: 18655025     EISSN: 18655033     Source Type: Journal    
DOI: 10.1007/s12195-014-0328-9     Document Type: Article

References (14)

1. Aslan, J. E., et al. The PAK system links Rho GTPase signaling to thrombin-mediated platelet activation. Am. J. Physiol. Cell Physiol. 305:C519-C528, 2013.
2. Aslan, J. E., G. W. Tormoen, C. P. Loren, J. Pang, and O. J. T. McCarty. S6K1 and mTOR regulate Rac1-driven platelet activation and aggregation. Blood 118:3129-3136, 2011.
3. Baker, S. M., K. G. Phillips, and O. J. T. McCarty. Development of a label-free imaging technique for the quantification of thrombus formation. Cell. Mol. Bioeng. 5:488-492, 2012.
4. Baker-Groberg, S. M., K. G. Phillips, and O. J. T.McCarty. Quantification of volume, mass, and density of thrombus formation using brightfield and differential interference contrast microscopy. J. Biomed. Opt. 18:16014, 2013.
5. Hanke, A. A., et al. Impact of platelet count on results obtained from multiple electrode platelet aggregometry (Multiplate™). Eur. J. Med. Res. 15:214, 2010.
6. Maloney, S. F., L. F. Brass, and S. L. Diamond. P2Y12 or P2Y1 inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions. Integr. Biol. Quant. Biosci. Nano Macro 2:183-192, 2010.
7. McCarty, O. J. T., et al. Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow. J. Biol. Chem. 280:39474-39484, 2005. (Pubitemid 41713905)
8. Michelson, A. D. Platelet function testing in cardiovascular diseases. Circulation 110:e489-e493, 2004.
9. Phillips, K. G., et al. Quantification of cellular volume and sub-cellular density fluctuations: comparison of normal peripheral blood cells and circulating tumor cells identified in a breast cancer patient. Front. Cancer Mol. Targets Ther. 2:96, 2012.
10. Phillips, K. G., S. L. Jacques, and O. J. T. McCarty. Measurement of single cell refractive index, dry mass, volume, and density using a transillumination microscope. Phys. Rev. Lett. 109:118105, 2012.
11. Phillips, K. G., and O. J. T. McCarty. Optical quantification of cellular mass, volume, and density of circulating tumor cells identified in an ovarian cancer patient. Front. Cancer Mol. Targets Ther. 2:72, 2012.
12. Roberts, D. E., A. McNicol, and R. Bose. Mechanism of collagen activation in human platelets. J. Biol. Chem. 279:19421-19430, 2004. (Pubitemid 38623375)
13. Ruggeri, Z. M. Platelets in atherothrombosis. Nat. Med. 8:1227-1234, 2002. (Pubitemid 35373553)
14. Shattil, S. J., H. Kashiwagi, and N. Pampori. Integrin signaling: the platelet paradigm. Blood 91:2645-2657, 1998. (Pubitemid 28227512)