Total protein quantitation using the bicinchoninic acid assay and gradient elution moving boundary electrophoresis

Electrophoresis

Volumn 35, Issue 12-13, 2014, Pages 1887-1892

  Kralj, Jason G ^a   Munson, Matthew S ^a   Ross, David ^a  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

Author keywords

Bicinchoninic acid (BCA); Capacitively coupled contactless conductivity detection (C4D); Gradient elution moving boundary electrophoresis (GEMBE); Total protein assay

Indexed keywords

ASSAYS; CHEMICAL REACTIONS; COPPER COMPOUNDS; ELECTROPHORESIS;

BICINCHONINIC ACID; BICINCHONINIC ACIDS; CAPACITIVELY COUPLED CONTACTLESS CONDUCTIVITY DETECTION; CAPACITIVELY COUPLED CONTACTLESS CONDUCTIVITY DETECTION (C4D); GRADIENT ELUTION; GRADIENT ELUTION MOVING BOUNDARY ELECTROPHORESIS; MOVING BOUNDARY ELECTROPHORESIS; PROTEIN ASSAYS; TOTAL PROTEIN; TOTAL PROTEIN ASSAY;

PROTEINS;

BOVINE IMMUNOGLOBULIN; COPPER; INSULIN; TARTARIC ACID; BICINCHONINIC ACID; PROTEIN; QUINOLINE DERIVATIVE;

ARTICLE; BICINCHONINIC ACID ASSAY; CAPILLARY ELECTROPHORESIS; CHEMICAL REACTION; ELECTROPHORESIS; GRADIENT ELUTION MOVING BOUNDARY ELECTROPHORESIS; PH; PROTEIN ANALYSIS; PROTEIN DETERMINATION; REACTION TIME; SENSITIVITY ANALYSIS; SPECTROSCOPY; CHEMISTRY; ELECTRIC CONDUCTIVITY; LIMIT OF DETECTION; PROCEDURES;

ELECTRIC CONDUCTIVITY; ELECTROPHORESIS, CAPILLARY; LIMIT OF DETECTION; PROTEINS; QUINOLINES;

EID: 84903524551     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.201400025     Document Type: Article

References (18)

1. Krohn, R. I., Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] 2011, Appendix 3, 3H.
2. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., Klenk, D. C., Anal. Biochem. 1985, 150, 76-85.
3. Wang, H.-Y., Guo, C.-Y., Guo, C.-G., Fan, L.-Y., Zhang, L., Cao, C.-X., Anal. Chim. Acta 2013, 774, 92-99.
4. Guo, C.-Y., Wang, H.-Y., Liu, X.-P., Fan, L.-Y., Zhang, L., Cao, C.-X., Electrophoresis 2013, 34, 1343-1351.
5. Wang, H.-Y., Li, S., Tang, Y.-Y., Dong, J.-Y., Fan, L.-Y., Cao, C.-X., Analyst 2013, 138, 3544.
6. Meng, Q., Guo, L., Shen, R., Xie, J., Chin. J. Chromatog. 2010, 28, 682-687.
7. Shackman, J. G., Munson, M. S., Ross, D., Anal. Chem. 2007, 79, 565-571.
8. Ross, D., Electrophoresis 2010, 31, 3650-3657.
9. Ross, D., Electrophoresis 2010, 31, 3658-3664.
10. Strychalski, E. A., Henry, A. C., Ross, D., Anal. Chem. 2009, 81, 10201-10207.
11. Strychalski, E. A., Henry, A. C., Ross, D., Anal. Chem. 2011, 83, 6316-6322.
12. Kuban, P., Hauser, P. C., Electrophoresis 2009, 30, 176-188.
13. Kuban, P., Hauser, P. C., Electrophoresis 2011, 32, 30-42.
14. Kuban, P., Hauser, P. C., Electrophoresis 2013, 34, 55-69.
15. Epple, R., Blanes, L., Beavis, A., Roux, C., Doble, P., Electrophoresis 2010, 31, 2608-2613.
16. Wiechelman, K. J., Braun, R. D., Fitzpatrick, J. D., Anal. Biochem. 1988, 175, 231-237.
17. Ross, D., Kralj, J. G., Anal. Chem. 2008, 80, 9467-9474.
18. Huang, T., Long, M., Huo, B., Open Biomed. Eng. J. 2010, 4, 271-278.