Method for obtaining primary ovarian cancer cells from solid specimens

Journal of Visualized Experiments

Volumn , Issue 84, 2014, Pages

  Pribyl, Lee J ^a   Coughlin, Kathleen A ^a   Sueblinvong, Thanasak ^a   Shields, Kristin ^b   Iizuka, Yoshie ^a,c   Downs, Levi S ^a,c   Ghebre, Rahel G ^a,c   Bazzaro, Martina ^a,c  

^a UNIVERSITY OF MINNESOTA   (United States)

^b MARICOPA MEDICAL CENTER   (United States)

^c UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Clinical specimens; Downstream applications; Epithelial cultures; Issue 84; Medicine; Neoplasms; Ovarian cancer; Primary cell lines; Targeted therapies

Indexed keywords

ARTICLE; CYTOLOGY; FEMALE; HUMAN; METHODOLOGY; NEOPLASM; OVARY TUMOR; PATHOLOGY; TUMOR CELL CULTURE; TUMOR CELL LINE;

CELL LINE, TUMOR; CYTOLOGICAL TECHNIQUES; FEMALE; HUMANS; NEOPLASMS, GLANDULAR AND EPITHELIAL; OVARIAN NEOPLASMS; TUMOR CELLS, CULTURED;

EID: 84902671562     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51581     Document Type: Article

References (10)

1. Kurman R.J., Visvanathan K., Roden R., Wu T.C., Shih Ie M. Early detection and treatment of ovarian cancer: shifting from early stage to minimal volume of disease based on a new model of carcinogenesis. Am. J. Obstet. Gynecol. 198(4), 351-356, doi:10.1016/j.ajog.2008.01.005 (2008).
2. Kuhn E., Kurman R.J., Shih I.M. Ovarian Cancer Is an Imported Disease: Fact or Fiction? Curr. Obstet. Gynecol. Rep. 1(1), 1-9, doi:10.1007/s13669-001-004-1 (2012).
3. Sarojini S. et al. Early detection biomarkers for ovarian cancer. J. Oncol. 2012, doi:10.1155/2012/709049 (2012).
4. Shepherd T.G., Theriault B.L., Campbell E.J., Nachtigal M.W. Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients. Nat. Protoc. 1(6), 2643-2649, doi:10.1038/nprot.2006.328 (2006).
5. Tsao S.W. et al. Characterization of human ovarian surface epithelial cells immortalized by human papilloma viral oncogenes (HPV-E6E7 ORFs). Exp. Cell. Res. 218(2), 499-507, doi:10.1006/excr.1995.1184 (1995).
6. Auersperg N., Maines-Bandiera S.L., Dyck H.G., Kruk P.A. Characterization of cultured human ovarian surface epithelial cells: phenotypic plasticity and premalignant changes. Lab. Invest. 71(4), 510-518 (1994).
7. Brewer M. et al. In vitro model of normal, immortalized ovarian surface epithelial and ovarian cancer cells for chemoprevention of ovarian cancer. Gynecol. Oncol. 98(2), 182-192, doi:10.1016/j.ygyno.2005.01.051 (2005).
8. Sueblinvong T. et al. Establishment, characterization and downstream application of primary ovarian cancer cells derived from solid tumors. PLoS One.7(11), e50519, doi:10.1371/journal.pone.0050519 (2012).
9. Rockwell S. In vivo-in vitro tumour cell lines: characteristics and limitations as models for human cancer. Br. J. Cancer Suppl. 41(4), 118-122 (1980).
10. Wong C., Chen S. The development, application and limitations of breast cancer cell lines to study tamoxifen and aromatase inhibitor resistance. J. Steroid. Biochem. Mol. Biol. 131(3-5), 83-92, doi10.1016/j.jsbmb.2011.12.005 (2012).