New insights in heparin-induced thrombocytopenia by the use of fluid-phase assays to detect specifically platelet factor 4/heparin complex antibodies and antibody-secreting cells

Thrombosis Research

Volumn 134, Issue 1, 2014, Pages 174-181

  Schulze, Annika ^a   Jensch, Inga ^a   Krauel, Krystin ^a,b   Alahmad, Adnan ^a   Müller, Hans Peter ^a   Greinacher, Andreas ^b   Hundt, Matthias ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b UNIVERSITY MEDICINE GREIFSWALD   (Germany)

Author keywords

enzyme linked immunoassay; heparin; heparin induced thrombocytopenia; humoral immune response; mouse model; platelet factor 4

Indexed keywords

ANTIGEN; HEPARIN; IMMUNOGLOBULIN G; STREPTAVIDIN; THROMBOCYTE FACTOR 4; ANTIBODY;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY DETECTION; ANTIGEN BINDING; ARTICLE; BIOTINYLATION; BLOOD SAMPLING; CONTROLLED STUDY; CROSS REACTION; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FLUID PHASE ENZYME IMMUNOASSAY; HEPARIN INDUCED THROMBOCYTOPENIA; HUMAN; HUMORAL IMMUNITY; IMMUNIZATION; LYMPH VESSEL; MOUSE; NONHUMAN; PRIORITY JOURNAL; SECRETORY CELL; SPLEEN; ANIMAL; BLOOD; C57BL MOUSE; CHEMICALLY INDUCED; IMMUNOGLOBULIN PRODUCING CELL; IMMUNOLOGY; METABOLISM; THROMBOCYTOPENIA;

ANIMALS; ANTIBODIES; ANTIBODY-PRODUCING CELLS; FEMALE; HEPARIN; HUMANS; MICE; MICE, INBRED C57BL; PLATELET FACTOR 4; THROMBOCYTOPENIA;

EID: 84902549057     PISSN: 00493848     EISSN: 18792472     Source Type: Journal    
DOI: 10.1016/j.thromres.2014.04.014     Document Type: Article

References (31)

1. A. Greinacher, K. Althaus, K. Krauel, and S. Selleng Heparin-induced thrombocytopenia Hamostaseologie 30 17-8 2010 20 28
2. L. Ziporen, Z.Q. Li, K.S. Park, P. Sabnekar, W.Y. Liu, and G. Arepally et al. Defining an antigenic epitope on platelet factor 4 associated with heparin-induced thrombocytopenia Blood 92 1998 3250 3259
3. Z.Q. Li, W. Liu, K.S. Park, B.S. Sachais, G.M. Arepally, and D.B. Cines et al. Defining a second epitope for heparin-induced thrombocytopenia/thrombosis antibodies using KKO, a murine HIT-like monoclonal antibody Blood 99 2002 1230 1236
4. T.E. Warkentin, and J.G. Kelton Temporal aspects of heparin-induced thrombocytopenia N Engl J Med 344 2001 1286 1292
5. A. Greinacher, T. Kohlmann, U. Strobel, J.A. Sheppard, and T.E. Warkentin The temporal profile of the anti-PF4/heparin immune response Blood 113 2009 4970 4976
6. C. Potschke, S. Selleng, B.M. Broker, and A. Greinacher Heparin-induced thrombocytopenia: further evidence for a unique immune response Blood 120 2012 4238 4245
7. K. Krauel, C. Weber, S. Brandt, U. Zahringer, U. Mamat, and A. Greinacher et al. Platelet factor 4 binding to lipid A of Gram-negative bacteria exposes PF4/heparin-like epitopes Blood 120 2012 3345 3352
8. M.E. Jaax, K. Krauel, T. Marschall, S. Brandt, J. Gansler, and B. Furll et al. Complex formation with nucleic acids and aptamers alters the antigenic properties of platelet factor 4 Blood 122 2013 272 281
9. J.G. Kelton, and T.E. Warkentin Heparin-induced thrombocytopenia: a historical perspective Blood 112 2008 2607 2616
10. T.E. Warkentin HITlights: a career perspective on heparin-induced thrombocytopenia Am J Hematol 87 Suppl. 1 2012 S92 S99
11. A. Greinacher, J. Amiral, V. Dummel, A. Vissac, V. Kiefel, and C. Mueller-Eckhardt Laboratory diagnosis of heparin-associated thrombocytopenia and comparison of platelet aggregation test, heparin-induced platelet activation test, and platelet factor 4/heparin enzyme-linked immunosorbent assay Transfusion 34 1994 381 385
12. D. Juhl, P. Eichler, N. Lubenow, U. Strobel, A. Wessel, and A. Greinacher Incidence and clinical significance of anti-PF4/heparin antibodies of the IgG, IgM, and IgA class in 755 consecutive patient samples referred for diagnostic testing for heparin-induced thrombocytopenia Eur J Haematol 76 2006 420 426
13. G. Arepally, C. Reynolds, A. Tomaski, J. Amiral, A. Jawad, and M. Poncz et al. Comparison of PF4/heparin ELISA assay with the 14C-serotonin release assay in the diagnosis of heparin-induced thrombocytopenia Am J Clin Pathol 104 1995 648 654
14. S. Suvarna, R. Qi, and G.M. Arepally Optimization of a murine immunization model for study of PF4/heparin antibodies J Thromb Haemost 7 2009 857 864
15. R. Jemmerson Antigenicity and native structure of globular proteins: low frequency of peptide reactive antibodies Proc Natl Acad Sci U S A 84 1987 9180 9184
16. V.V. Hlady, and J. Buijs Protein adsorption on solid surfaces Curr Opin Biotechnol 7 1996 72 77
17. P.M. Newman, R.L. Swanson, and B.H. Chong Heparin-induced thrombocytopenia: IgG binding to PF4-heparin complexes in the fluid phase and cross-reactivity with low molecular weight heparin and heparinoid Thromb Haemost 80 1998 292 297
18. K. Selleng, A. Schutt, S. Selleng, T.E. Warkentin, and A. Greinacher Studies of the anti-platelet factor 4/heparin immune response: adapting the enzyme-linked immunosorbent spot assay for detection of memory B cells against complex antigens Transfusion 50 2010 32 39
19. T. Yardeni, M. Eckhaus, H.D. Morris, M. Huizing, and S. Hoogstraten-Miller Retro-orbital injections in mice Lab Anim (NY) 40 2011 155 160
20. S. Suvarna, L. Rauova, E.K. McCracken, C.M. Goss, B.S. Sachais, and S.E. McKenzie et al. PF4/heparin complexes are T cell-dependent antigens Blood 106 2005 929 931
21. K. Althaus, G. Hron, U. Strobel, R. Abbate, A. Rogolino, and S. Davidson et al. Evaluation of automated immunoassays in the diagnosis of heparin induced thrombocytopenia Thromb Res 131 2013 e85 e90
22. D. Sheridan, C. Carter, and J.G. Kelton A diagnostic test for heparin-induced thrombocytopenia Blood 67 1986 27 30
23. K. Krauel, C. Potschke, C. Weber, W. Kessler, B. Furll, and T. Ittermann et al. Platelet factor 4 binds to bacteria, [corrected] inducing antibodies cross-reacting with the major antigen in heparin-induced thrombocytopenia Blood 117 2011 1370 1378
24. K. Althaus, U. Strobel, T.E. Warkentin, and A. Greinacher Combined use of the high heparin step and optical density to optimize diagnostic sensitivity and specificity of an anti-PF4/heparin enzyme-immunoassay Thromb Res 128 2011 256 260
25. Z.M. Doymaz, H. Bulut, A. Özdarendel, and Y. Bolat Production and Characterization of Monoclonal Anti-Ovalbumin Antibodies Turk J Med Sci 30 1999 411 416
26. P.M. Newman, and B.H. Chong Further characterization of antibody and antigen in heparin-induced thrombocytopenia Br J Haematol 107 1999 303 309
27. L. Yu, J. Wang, J.R. O'Dell, J. Oates, W.P. Arend, and G.S. Eisenbarth Anti-dsDNA antibody assay: high specificity and sensitivity with a filtration radioassay in comparison to low specificity with the standard ELISA J Rheumatol 34 2007 734 739
28. Y. Zheng, A.W. Wang, M. Yu, A. Padmanabhan, B.E. Tourdot, and D.K. Newman et al. B-cell tolerance regulates production of antibodies causing heparin-induced thrombocytopenia Blood 123 2014 931 934
29. Y. Zheng, M. Yu, A. Podd, L. Yuan, D.K. Newman, and R. Wen et al. Critical role for mouse marginal zone B cells in PF4/heparin antibody production Blood 121 2013 3484 3492
30. J.S. Suh, R.H. Aster, and G.P. Visentin Antibodies from patients with heparin-induced thrombocytopenia/thrombosis recognize different epitopes on heparin: platelet factor 4 Blood 91 1998 916 922
31. K.D. Salazar, P. de la Rosa, J.B. Barnett, and R. Schafer The polysaccharide antibody response after streptococcus pneumoniae vaccination is differentially enhanced or suppressed by 3,4-dichloropropionanilide and 2,4-dichlorophenoxyacetic acid Toxicol Sci 87 2005 123 133