Microfabricated droplet generator for single molecule PCR in tunable, monodisperse emulsions

Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Volumn , Issue , 2007, Pages 949-951

  Kumaresan, P ^a   Yang, C J ^a   Blazej, R G ^a   Mathies, R A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Droplet generation; Microemulsion PCR; Single molecule PCR

Indexed keywords

DROP BREAKUP; DROP FORMATION; EMULSIFICATION; EMULSIONS; MICROFABRICATION; OSTWALD RIPENING; SPRAY NOZZLES;

DROPLET GENERATORS; EMULSION DROPLETS; GENETIC ANALYSIS; GENOMIC ANALYSIS; MONODISPERSE DROPLETS; MONODISPERSE EMULSIONS; NEXT-GENERATION SEQUENCING; NOZZLE STRUCTURE;

POLYMERASE CHAIN REACTION;

EID: 84902441629     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (4)

1. M. Margulies et al., “Genome sequencing in microfabricated high-density picoliter reactors”, Nature, Vol. 437, pp. 376-380, (2005).
2. R.G. Blazej, P. Kumaresan, R.A. Mathies, “Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing”, Proc. Natl. Acad. Sci. USA, Vol. 103, pp. 7240-7245, (2006).
3. R.G. Blazej, P. Kumaresan, S.A. Cronier, R.A. Mathies, “Inline-injection microdevice for attomole-scale Sanger DNA sequencing”, Anal. Chem., In press.
4. E.M. Chan, A.P. Alivosatos, R.A. Mathies, “High-temperature microfluidic synthesis of CdSe nanocrystals in nanoliter droplets”, J. Am. Chem. Soc., Vol. 127, pp. 13854-13861, (2005).