Simultaneous detection of four causal agents of tobacco bushy top disease by a multiplex one-step RT-PCR

Journal of Virological Methods

Volumn 205, Issue , 2014, Pages 99-103

  Liu, Fang ^a   Tan, Guanlin ^a   Li, Xiaojing ^a   Chen, Hairu ^a   Li, Ruhui ^b   Li, Fan ^a  

^a YUNNAN AGRICULTURAL UNIVERSITY   (China)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Multiplex RT PCR; Sat TBTV; TBTV; Tobacco bushy top disease; TVDV; TVDVaRNA

Indexed keywords

SATELLITE RNA OF TOBACCO BUSHY TOP VIRUS; TOBACCO VEIN DISTORTING VIRUS ASSOCIATE RNA; UNCLASSIFIED DRUG; VIRUS RNA; PRIMER DNA;

APHID; ARTICLE; CHINA; CONTROLLED STUDY; LIMIT OF DETECTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PLANT VIRUS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TOBACCO; TOBACCO BUSHY TOP VIRUS; TOBACCO VEIN DISTORTING VIRUS; VIRUS DETECTION; VIRUS TRANSMISSION; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; LUTEOVIRIDAE; PLANT DISEASE; PROCEDURES; REPRODUCIBILITY; SPECIES DIFFERENCE; TOMBUSVIRIDAE; VIROLOGY;

DNA PRIMERS; LUTEOVIRIDAE; MULTIPLEX POLYMERASE CHAIN REACTION; PLANT DISEASES; PLANT VIRUSES; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TOBACCO; TOMBUSVIRIDAE;

EID: 84902006830     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.05.003     Document Type: Article

References (19)

1. Blancard D., Delon R., Blair B.W., Glover T. Major tobacco diseases: virus diseases. Tobacco: Production, Chemistry and Technology 1999, 198-215. Blackwell Science, Oxford, UK. D.L. Davis, M.T. Nielsen (Eds.).
2. Cole J.S. Isolation of tobacco vein distorting virus from tobacco plants infected with aphid-transmissible bushy top. Phytopathology 1962, 52:1312.
3. Deb M., Anderson J.M. Development of a multiplexed PCR detection method for Barley and Cereal yellow dwarf viruses, wheat spindle streak virus. Wheat streak mosaic virus and Soil-borne wheat mosaic virus. J. Virol. Methods 2008, 148:17-24.
4. Elnifro E.M., Ashshi A.M., Cooper R.J., Klapper P.E. Multiplex PCR: optimization and application in diagnostic virology. Clin. Microbiol. Rev. 2000, 13(4):559-570.
5. Gambino G., Gribaudo I. Simultaneous detection of nine grapevine viruses by multiplex reverse transcription-polymerase chain reaction with coamplification of a plant RNA as internal control. Phytopathology 2006, 96(11):1223-1229.
6. Gates L.F. A virus causing axi11ary bud sprouting of tobacco in Rhodesia and Nyasaland. Ann. Appl. Biol. 1962, 50:169-174.
7. Li F., Chen J.Y., Wu J.Y., Qian N.G., Wang Y.L., Chen H.R. An improved rapid diagnosis method of tobacco bushy top disease. Plant Quarantine 2005, 19(5):278-280. (in Chinese).
8. Li F., Wu J.Y., Chen H.R. Advances of research on tobacco bushy top disease. Acta Phytopathol. Sinica 2005, 35:385-391. (in Chinese).
9. Li F., Zuo R., Abad J., Xu D., Bao G., Li R. Simultaneous detection and differentiation of four closely related sweet potato potyviruses by a multiplex one-step RT-PCR. J. Virol. Methods 2012, 186:161-166.
10. Li R., Mock R., Huang Q., et al. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. J. Virol. Methods 2008, 154:48-55.
11. Ma Z., Michailides T.J. Approaches for eliminating PCR inhibitors and designing PCR primers for the detection of phytopathogenic fungi. Crop Prot. 2007, 26:145-161.
12. Mo X.H., Chen Z.B., Chen J.P. Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus. Virus Genes 2010, 41:425-431.
13. Mo X.H., Chen Z.B., Chen J.P. Molecular identification and phylogenetic analysis of a viral RNA associated with the Chinese tobacco bushy top disease complex. Ann. Appl. Biol. 2011, 158:188-193.
14. Mo X.H., Qin X.Y., Wu J., Yang C., Wu J.Y., Duan Y.Q., Li T.F., Chen H.R. Complete nucleotide sequence and genome organization of a Chinese isolate of tobacco bushy top virus. Arch. Virol. 2003, 148:389-397.
15. Ndowora N. International research fellowship report. Microbiol. Today 2002, 29:100-101.
16. Sánchez-Navarro J.A., Aparicio F., Herranz M.C., Minafra A., Myrta A., Pallás V. Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. Eur. J. Plant Pathol. 2005, 111(1):77-84.
17. Viganó F., Stevens M. Development of a multiplex immunocapture-RT-PCR for simultaneous detection of BMYV and BChV in plants and single aphids. J. Virol. Methods 2007, 146:196-201.
18. Wei T., Lu G., Clover G. A mutiplex RT-PCR for the detection of Potato yellow vein virus, Tobacco rattle virus and Tomato infectious chlorosis virus in potato with a plant intemal amplification control. Plant Pathol. 2009, 58(2):203-209.
19. Xie Y., Wang M., Xu D., Li R., Zhou G. Simultaneous detection and identification of four sugarcane viruses by one-step RT-PCR. J. Virol. Methods 2009, 162:64-68.