Surface histidine mutations for the metal affinity purification of a β-carbonic anhydrase

Analytical Biochemistry

Volumn 458, Issue , 2014, Pages 66-68

  Hoffmann, Katherine M ^a   Wood, Kaitlin M ^a   Labrum, Alysha D ^a   Lee, Dave K ^a   Bolinger, Ingmar M ^a   Konis, Mary E ^a   Blount, Adam G ^a   Prussia, Gregory A ^a   Schroll, Monica M ^a   Watson, Jeffrey M ^a  

^a GONZAGA UNIVERSITY   (United States)

Author keywords

Endogenous nickel affinity; Haemophilus influenzae; Immobilized metal affinity purification; Surface histidines; Carbonic anhydrase

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ESCHERICHIA COLI; METALS; PURIFICATION;

CELLULAR SYSTEM; HAEMOPHILUS INFLUENZAE; IMMOBILIZED METALS; LABELING TECHNIQUES; LABORATORY TECHNIQUES; METAL-AFFINITY CHROMATOGRAPHY; NICKEL AFFINITIES; SURFACE CHARACTER;

CARBONIC ANHYDRASE;

BACTERIAL ENZYME; BETA CARBONATE DEHYDRATASE; CARBONATE DEHYDRATASE; HISTIDINE; UNCLASSIFIED DRUG; ARGININE;

ARTICLE; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; FLAME PHOTOMETRY; HAEMOPHILUS INFLUENZAE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN QUATERNARY STRUCTURE; STEADY STATE; SURFACE PROPERTY; AFFINITY CHROMATOGRAPHY; AMINO ACID SUBSTITUTION; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM;

AMINO ACID SUBSTITUTION; ARGININE; CARBONIC ANHYDRASES; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE; HISTIDINE; KINETICS; SURFACE PROPERTIES;

EID: 84901941588     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.04.020     Document Type: Article

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