Probing the ubiquinol-binding site of recombinant Sauromatum guttatum alternative oxidase expressed in E. coli membranes through site-directed mutagenesis

Biochimica et Biophysica Acta - Bioenergetics

Volumn 1837, Issue 7, 2014, Pages 1219-1225

  Young, Luke ^a   May, Benjamin ^a   Pendlebury Watt, Alice ^a   Shearman, Julia ^a   Elliott, Catherine ^a   Albury, Mary S ^a   Shiba, Tomoo ^b   Inaoka, Daniel Ken ^c   Harada, Shigeharu ^b   Kita, Kiyoshi ^c   Moore, Anthony L ^a  

^a UNIVERSITY OF SUSSEX   (United Kingdom)

^b KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

Alternative oxidase; AOX inhibitors; Escherichia coli membrane; Oxygen affinity; Quinol oxidase; Site directed mutagenesis; Structure function relation

Indexed keywords

ALTERNATIVE OXIDASE; OXIDOREDUCTASE; RECOMBINANT ENZYME; UBIQUINONE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ESCHERICHIA COLI; HYDROPHOBICITY; LIPID BILAYER; MEMBRANE BINDING; NONHUMAN; OXYGEN CONSUMPTION; PLANT; PRIORITY JOURNAL; PROTEIN EXPRESSION; SAUROMATUM GUTTATUM; SITE DIRECTED MUTAGENESIS; STEADY STATE; TRYPANOSOMIASIS; UBIQUINOL BINDING SITE;

ESCHERICHIA COLI; SAUROMATUM GUTTATUM;

ALTERNATIVE OXIDASE; AOX INHIBITORS; ESCHERICHIA COLI MEMBRANE; OXYGEN AFFINITY; QUINOL OXIDASE; SITE-DIRECTED MUTAGENESIS; STRUCTURE-FUNCTION RELATION;

AMINO ACID SEQUENCE; ARACEAE; BINDING SITES; CELL MEMBRANE; ESCHERICHIA COLI; MITOCHONDRIAL PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; OXIDOREDUCTASES; PLANT PROTEINS; RECOMBINANT PROTEINS; TERPENES; UBIQUINONE;

EID: 84901820878     PISSN: 00052728     EISSN: 18792650     Source Type: Journal    
DOI: 10.1016/j.bbabio.2014.01.027     Document Type: Article

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