High-yield electroporation of cells using field constriction at micro orifices

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Volumn 1, Issue , 2005, Pages 1401-1403

  Washizu, Masao ^a   Wake, Yoshifumi ^a   Kurosawa, Osamu ^b   Oana, Hidehiro ^a   Matsuoka, Satoshi ^c   Noma, Akinori ^c   Kotera, Hidetoshi ^d  

^a UNIVERSITY OF TOKYO   (Japan)

^b Advance Co   (Japan)

^c Dept of Physiology and Biophysics ^*  (Japan)

^d KYOTO UNIVERSITY   (Japan)

Author keywords

Cell; Electroporation; Field constriction; Orifice; Transfection

Indexed keywords

CELL MEMBRANES; CELLS; CHEMICAL ANALYSIS; ORIFICES;

ELECTROPORATION; FIELD CONSTRICTION; HIGH-EFFICIENCY; MICRO-ORIFICE; MICROORIFICES; NONUNIFORM FIELD; PULSED VOLTAGES; TRANSFECTION;

MOLECULAR BIOLOGY;

EID: 84901776866     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (2)

1. U.Zimmermann: "Electrical breakdown, electropermeabilization and electrofusion", Rev. Physiol. Biochem. Pharmacol. 15, p.175-345 (1986).
2. A. Tixier-Mita et al: "A silicon micro-system for parallel gene transfection into arrayed cells", Proc. μ-TAS 2004, Vol.2, p.180-182 (2004).