Protein products obtained by site-preferred partial crosslinking in protein crystals and "liberated" by redissolution

Biotechnology and Bioengineering

Volumn 111, Issue 7, 2014, Pages 1296-1303

  Buch, Michal ^a   Wine, Yariv ^a   Dror, Yael ^a   Rosenheck, Sonia ^a   Lebendiker, Mario ^b   Giordano, Rita ^c   Leal, Ricardo M F ^d   Popov, Alexander N ^e   Freeman, Amihay ^a   Frolow, Felix ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c PAUL SCHERRER INSTITUTE   (Switzerland)

^d EUROPEAN SYNCHROTRON RADIATION FACILITY   (France)

^e INSTITUT LAUE LANGEVIN   (France)

Author keywords

Crosslinking; Glutaraldehyde; Protein crystals; Structure analysis; X ray diffraction

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; DIMERS; MOLECULES; PROTEINS; X RAY DIFFRACTION;

CHEMICAL CROSS-LINKING; CHROMATOGRAPHIC PURIFICATION; CROSSLINKED PRODUCTS; CROSSLINKING REACTION; GLUTARALDEHYDES; PROTEIN CRYSTAL; SINGLE PROTEIN MOLECULES; STRUCTURE ANALYSIS;

CROSSLINKING;

DIMER; GLUTARALDEHYDE; LYSOZYME; MONOMER; PROTEIN;

ARTICLE; CRYSTALLIZATION; DISSOLUTION; ENZYME ACTIVITY; ION EXCHANGE CHROMATOGRAPHY; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN CROSS LINKING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; CHEMISTRY; PROTEIN STABILITY;

CRYSTALLIZATION; PROTEIN STABILITY; PROTEINS;

EID: 84901631993     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25186     Document Type: Article

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