메뉴 건너뛰기




Volumn 80, Issue 12, 2014, Pages 3597-3603

Utility of the clostridial site-specific recombinase TnpX to clone toxic-product-encoding genes and selectively remove Genomic DNA fragments

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; CLOSTRIDIUM; ENCODING (SYMBOLS); ESCHERICHIA COLI;

EID: 84901353487     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.04285-13     Document Type: Article
Times cited : (8)

References (42)
  • 1
    • 34548124567 scopus 로고    scopus 로고
    • The ClosTron: a universal gene knock-out system for the genus Clostridium
    • Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. 2007. The ClosTron: a universal gene knock-out system for the genus Clostridium. J. Microbiol. Methods 70:452-464. http://dx.doi.org/10.1016/j.mimet.2007.05.021.
    • (2007) J. Microbiol. Methods , vol.70 , pp. 452-464
    • Heap, J.T.1    Pennington, O.J.2    Cartman, S.T.3    Carter, G.P.4    Minton, N.P.5
  • 2
    • 0037444087 scopus 로고    scopus 로고
    • Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker
    • Zhong J, Karberg M, Lambowitz AM. 2003. Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker. Nucleic Acids Res. 31:1656-1664. http://dx.doi.org/10.1093/nar/gkg248.
    • (2003) Nucleic Acids Res , vol.31 , pp. 1656-1664
    • Zhong, J.1    Karberg, M.2    Lambowitz, A.M.3
  • 3
    • 32044466694 scopus 로고    scopus 로고
    • Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron
    • Chen Y, McClane BA, Fisher DJ, Rood JI, Gupta P. 2005. Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron. Appl. Environ. Microbiol. 71:7542- 7547. http://dx.doi.org/10.1128/AEM.71.11.7542-7547.2005.
    • (2005) Appl. Environ. Microbiol. , vol.71 , pp. 7542-7547
    • Chen, Y.1    McClane, B.A.2    Fisher, D.J.3    Rood, J.I.4    Gupta, P.5
  • 4
    • 84877696851 scopus 로고    scopus 로고
    • ClosTron-mediated engineering of Clostridium
    • Kuehne SA, Minton NP. 2012. ClosTron-mediated engineering of Clostridium. Bioengineered 3:247-254. http://dx.doi.org/10.4161/bioe.21004.
    • (2012) Bioengineered , vol.3 , pp. 247-254
    • Kuehne, S.A.1    Minton, N.P.2
  • 5
    • 84891929627 scopus 로고    scopus 로고
    • Spo0A differentially regulates toxin production in evolutionarily diverse strains of Clostridium difficile
    • Mackin KE, Carter GP, Howarth P, Rood JI, Lyras D. 2013. Spo0A differentially regulates toxin production in evolutionarily diverse strains of Clostridium difficile. PLoS One 8:e79666. http://dx.doi.org/10.1371 /journal.pone.0079666.
    • (2013) PLoS One , vol.8
    • Mackin, K.E.1    Carter, G.P.2    Howarth, P.3    Rood, J.I.4    Lyras, D.5
  • 6
    • 0029065955 scopus 로고
    • Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant
    • Cherepanov PP, Wackernagel W. 1995. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158:9-14. http://dx.doi.org /10.1016/0378-1119(95)00193-A.
    • (1995) Gene , vol.158 , pp. 9-14
    • Cherepanov, P.P.1    Wackernagel, W.2
  • 8
    • 84868649467 scopus 로고    scopus 로고
    • Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration
    • Al-Hinai MA, Fast AG, Papoutsakis ET. 2012. Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration. Appl. Environ. Microbiol. 78:8112-8121. http://dx.doi.org/10.1128/AEM.02214-12.
    • (2012) Appl. Environ. Microbiol. , vol.78 , pp. 8112-8121
    • Al-Hinai, M.A.1    Fast, A.G.2    Papoutsakis, E.T.3
  • 9
    • 0036969318 scopus 로고    scopus 로고
    • The clostridial mobilisable transposons
    • Adams V, Lyras D, Farrow K, Rood J. 2002. The clostridial mobilisable transposons. Cell. Mol. Life Sci. 59:2033-2043. http://dx.doi.org/10.1007 /s000180200003.
    • (2002) Cell. Mol. Life Sci. , vol.59 , pp. 2033-2043
    • Adams, V.1    Lyras, D.2    Farrow, K.3    Rood, J.4
  • 10
    • 0023212832 scopus 로고
    • Identification of Tn4451 and Tn4452, chloramphenicol resistance transposons from Clostridium perfringens
    • Abraham LJ, Rood JI. 1987. Identification of Tn4451 and Tn4452, chloramphenicol resistance transposons from Clostridium perfringens. J. Bacteriol. 169:1579-1584.
    • (1987) J. Bacteriol. , vol.169 , pp. 1579-1584
    • Abraham, L.J.1    Rood, J.I.2
  • 11
    • 0031808781 scopus 로고    scopus 로고
    • Chloramphenicol resistance in Clostridium difficile is encoded on Tn4453 transposons that are closely related to Tn4451 from Clostridium perfringens
    • Lyras D, Storie C, Huggins AS, Crellin PK, Bannam TL, Rood JI. 1998. Chloramphenicol resistance in Clostridium difficile is encoded on Tn4453 transposons that are closely related to Tn4451 from Clostridium perfringens. Antimicrob. Agents Chemother. 42:1563-1567.
    • (1998) Antimicrob. Agents Chemother. , vol.42 , pp. 1563-1567
    • Lyras, D.1    Storie, C.2    Huggins, A.S.3    Crellin, P.K.4    Bannam, T.L.5    Rood, J.I.6
  • 12
    • 1642483709 scopus 로고    scopus 로고
    • The large resolvase TnpX is the only transposon-encoded protein required for transposition of the Tn4451/3 family of integrative mobilizable elements
    • Lyras D, Adams V, Lucet I, Rood JI. 2004. The large resolvase TnpX is the only transposon-encoded protein required for transposition of the Tn4451/3 family of integrative mobilizable elements. Mol. Microbiol. 51: 1787-1800. http://dx.doi.org/10.1111/j.1365-2958.2003.03950.x.
    • (2004) Mol. Microbiol. , vol.51 , pp. 1787-1800
    • Lyras, D.1    Adams, V.2    Lucet, I.3    Rood, J.I.4
  • 13
    • 4344673752 scopus 로고    scopus 로고
    • DNA binding properties of TnpX indicate that different synapses are formed in the excision and integration of the Tn4451 family
    • Adams V, Lucet IS, Lyras D, Rood JI. 2004. DNA binding properties of TnpX indicate that different synapses are formed in the excision and integration of the Tn4451 family. Mol. Microbiol. 53:1195-1207. http://dx.doi.org/10.1111/j.1365-2958.2004.04198.x.
    • (2004) Mol. Microbiol. , vol.53 , pp. 1195-1207
    • Adams, V.1    Lucet, I.S.2    Lyras, D.3    Rood, J.I.4
  • 14
    • 0033710355 scopus 로고    scopus 로고
    • Transposition of Tn4451 and Tn4453 involves a circular intermediate that forms a promoter for the large resolvase
    • Lyras D, Rood J. 2000. Transposition of Tn4451 and Tn4453 involves a circular intermediate that forms a promoter for the large resolvase, TnpX. Mol. Microbiol. 38:588-601. http://dx.doi.org/10.1046/j.1365-2958.2000.02154.x.
    • (2000) TnpX. Mol. Microbiol. , vol.38 , pp. 588-601
    • Lyras, D.1    Rood, J.2
  • 15
    • 79961004324 scopus 로고    scopus 로고
    • Clostridium difficile has two parallel and essential Sec secretion systems
    • Fagan RP, Fairweather NF. 2011. Clostridium difficile has two parallel and essential Sec secretion systems. J. Biol. Chem. 286:27483-27493. http://dx.doi.org/10.1074/jbc.M111.263889.
    • (2011) J. Biol. Chem. , vol.286 , pp. 27483-27493
    • Fagan, R.P.1    Fairweather, N.F.2
  • 17
    • 0022529269 scopus 로고
    • Promoter-probe vectors for the analysis of divergently arranged promoters
    • Schneider K, Beck CF. 1986. Promoter-probe vectors for the analysis of divergently arranged promoters. Gene 42:37-48. http://dx.doi.org/10.1016/0378-1119(86)90148-4.
    • (1986) Gene , vol.42 , pp. 37-48
    • Schneider, K.1    Beck, C.F.2
  • 18
    • 0028225456 scopus 로고
    • Identification and molecular analysis of a locus that regulates extracellular toxin production in Clostridium perfringens
    • Lyristis M, Bryant AE, Sloan J, Awad MM, Nisbet IT, Stevens DL, Rood JI. 1994. Identification and molecular analysis of a locus that regulates extracellular toxin production in Clostridium perfringens. Mol. Microbiol. 12:761-777. http://dx.doi.org/10.1111/j.1365-2958.1994.tb01063.x.
    • (1994) Mol. Microbiol. , vol.12 , pp. 761-777
    • Lyristis, M.1    Bryant, A.E.2    Sloan, J.3    Awad, M.M.4    Nisbet, I.T.5    Stevens, D.L.6    Rood, J.I.7
  • 19
    • 84858996693 scopus 로고    scopus 로고
    • The peptidoglycan hydrolase TcpG is required for efficient conjugative transfer of pCW3 in Clostridium perfringens
    • Bantwal R, Bannam TL, Porter CJ, Quinsey NS, Lyras D, Adams V, Rood JI. 2012. The peptidoglycan hydrolase TcpG is required for efficient conjugative transfer of pCW3 in Clostridium perfringens. Plasmid 67:139- 147. http://dx.doi.org/10.1016/j.plasmid.2011.12.016.
    • (2012) Plasmid , vol.67 , pp. 139-147
    • Bantwal, R.1    Bannam, T.L.2    Porter, C.J.3    Quinsey, N.S.4    Lyras, D.5    Adams, V.6    Rood, J.I.7
  • 20
    • 0021875997 scopus 로고
    • Cloning and analysis of the Clostridium perfringens tetracycline resistance plasmid, pCW3
    • Abraham LJ, Rood JI. 1985. Cloning and analysis of the Clostridium perfringens tetracycline resistance plasmid, pCW3. Plasmid 13:155-162. http://dx.doi.org/10.1016/0147-619X(85)90038-1.
    • (1985) Plasmid , vol.13 , pp. 155-162
    • Abraham, L.J.1    Rood, J.I.2
  • 21
    • 0025778670 scopus 로고
    • Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives
    • Bartolomé B, Jubete Y, Martinez E, de la Cruz F. 1991. Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene 102:75-78. http://dx.doi.org/10.1016/0378-1119(91)90541-I.
    • (1991) Gene , vol.102 , pp. 75-78
    • Bartolomé, B.1    Jubete, Y.2    Martinez, E.3    de la Cruz, F.4
  • 22
    • 0027263622 scopus 로고
    • Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants
    • Bannam TL, Rood JI. 1993. Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. Plasmid 29:233-235.
    • (1993) Plasmid , vol.29 , pp. 233-235
    • Bannam, T.L.1    Rood, J.I.2
  • 23
    • 0031894178 scopus 로고    scopus 로고
    • Conjugative transfer of RP4-oriT shuttle vectors from Escherichia coli to Clostridium perfringens
    • Lyras D, Rood JI. 1998. Conjugative transfer of RP4-oriT shuttle vectors from Escherichia coli to Clostridium perfringens. Plasmid 39:160-164. http: //dx.doi.org/10.1006/plas.1997.1325.
    • (1998) Plasmid , vol.39 , pp. 160-164
    • Lyras, D.1    Rood, J.I.2
  • 24
    • 33645827332 scopus 로고    scopus 로고
    • Two distinct regions of the large serine recombinase TnpX are required for DNA binding and biological function
    • Adams V, Lucet IS, Tynan FE, Chiarezza M, Howarth PM, Kim J, Rossjohn J, Lyras D, Rood JI. 2006. Two distinct regions of the large serine recombinase TnpX are required for DNA binding and biological function. Mol. Microbiol. 60:591-601. http://dx.doi.org/10.1111/j.1365 -2958.2006.05120.x.
    • (2006) Mol. Microbiol. , vol.60 , pp. 591-601
    • Adams, V.1    Lucet, I.S.2    Tynan, F.E.3    Chiarezza, M.4    Howarth, P.M.5    Kim, J.6    Rossjohn, J.7    Lyras, D.8    Rood, J.I.9
  • 26
    • 77953940736 scopus 로고    scopus 로고
    • The VirSR two-component signal transduction system regulates NetB toxin production in Clostridium perfringens
    • Cheung JK, Keyburn AL, Carter GP, Lanckriet AL, Van Immerseel F, Moore RJ, Rood JI. 2010. The VirSR two-component signal transduction system regulates NetB toxin production in Clostridium perfringens. Infect. Immun. 78:3064-3072. http://dx.doi.org/10.1128/IAI.00123-10.
    • (2010) Infect. Immun. , vol.78 , pp. 3064-3072
    • Cheung, J.K.1    Keyburn, A.L.2    Carter, G.P.3    Lanckriet, A.L.4    Van Immerseel, F.5    Moore, R.J.6    Rood, J.I.7
  • 28
    • 0017903276 scopus 로고
    • Isolation and characterization of multiply antibiotic-resistant Clostridium perfringens strains from porcine feces
    • Rood JI, Maher EA, Somers EB, Campos E, Duncan CL. 1978. Isolation and characterization of multiply antibiotic-resistant Clostridium perfringens strains from porcine feces. Antimicrob. Agents Chemother. 13:871- 880. http://dx.doi.org/10.1128/AAC.13.5.871.
    • (1978) Antimicrob. Agents Chemother. , vol.13 , pp. 871-880
    • Rood, J.I.1    Maher, E.A.2    Somers, E.B.3    Campos, E.4    Duncan, C.L.5
  • 29
    • 0021017779 scopus 로고
    • Transferable tetracycline resistance in Clostridium perfringens strains of porcine origin
    • Rood JI. 1983. Transferable tetracycline resistance in Clostridium perfringens strains of porcine origin. Can. J. Microbiol. 29:1241-1246. http://dx.doi.org/10.1139/m83-193.
    • (1983) Can. J. Microbiol. , vol.29 , pp. 1241-1246
    • Rood, J.I.1
  • 31
    • 0024436683 scopus 로고
    • Electroporation-mediated transformation of lysostaphin-treated Clostridium perfringens
    • Scott PT, Rood JI. 1989. Electroporation-mediated transformation of lysostaphin-treated Clostridium perfringens. Gene 82:327-333. http://dx.doi.org/10.1016/0378-1119(89)90059-0.
    • (1989) Gene , vol.82 , pp. 327-333
    • Scott, P.T.1    Rood, J.I.2
  • 34
    • 0034769457 scopus 로고    scopus 로고
    • Genomic analysis of the erythromycin resistance element Tn5398 from Clostridium difficile
    • Farrow KA, Lyras D, Rood JI. 2001. Genomic analysis of the erythromycin resistance element Tn5398 from Clostridium difficile. Microbiology 147:2717-2728. http://mic.sgmjournals.org/content/147/10/2717.long.
    • (2001) Microbiology , vol.147 , pp. 2717-2728
    • Farrow, K.A.1    Lyras, D.2    Rood, J.I.3
  • 36
    • 0024556150 scopus 로고
    • Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
    • Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR. 1989. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68. http://dx.doi.org/10.1016/0378-1119(89) 90359-4.
    • (1989) Gene , vol.77 , pp. 61-68
    • Horton, R.M.1    Hunt, H.D.2    Ho, S.N.3    Pullen, J.K.4    Pease, L.R.5
  • 37
    • 0028945951 scopus 로고
    • Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of α-toxin in Clostridium perfringens-mediated gas gangrene
    • Awad MM, Bryant AE, Stevens DL, Rood JI. 1995. Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of α-toxin in Clostridium perfringens-mediated gas gangrene. Mol. Microbiol. 15:191-202. http://dx.doi.org/10.1111/j.1365-2958.1995.tb02234.x.
    • (1995) Mol. Microbiol. , vol.15 , pp. 191-202
    • Awad, M.M.1    Bryant, A.E.2    Stevens, D.L.3    Rood, J.I.4
  • 38
    • 0028203277 scopus 로고
    • The virR gene, a member of a class of two-component response regulators, regulates the production of perfringolysin O, collagenase, and hemagglutinin in Clostridium perfringens
    • Shimizu T, Ba-Thein W, Tamaki M, Hayashi H. 1994. The virR gene, a member of a class of two-component response regulators, regulates the production of perfringolysin O, collagenase, and hemagglutinin in Clostridium perfringens. J. Bacteriol. 176:1616-1623.
    • (1994) J. Bacteriol. , vol.176 , pp. 1616-1623
    • Shimizu, T.1    Ba-Thein, W.2    Tamaki, M.3    Hayashi, H.4
  • 39
    • 33749264645 scopus 로고    scopus 로고
    • Major role for FeoB in Campylobacter jejuni ferrous iron acquisition, gut colonization, and intracellular survival
    • Naikare H, Palyada K, Panciera R, Marlow D, Stintzi A. 2006. Major role for FeoB in Campylobacter jejuni ferrous iron acquisition, gut colonization, and intracellular survival. Infect. Immun. 74:5433-5444. http://dx.doi.org/10.1128/IAI.00052-06.
    • (2006) Infect. Immun. , vol.74 , pp. 5433-5444
    • Naikare, H.1    Palyada, K.2    Panciera, R.3    Marlow, D.4    Stintzi, A.5
  • 40
    • 84886054254 scopus 로고    scopus 로고
    • FeoA and FeoC are essential components of Vibrio cholerae ferrous iron uptake system, and FeoC interacts with FeoB
    • Weaver EA, Wyckoff EE, Mey AR, Morrison R, Payne SM. 2013. FeoA and FeoC are essential components of Vibrio cholerae ferrous iron uptake system, and FeoC interacts with FeoB. J. Bacteriol. 195:4826-4835. http: //dx.doi.org/10.1128/JB.00738-13.
    • (2013) J. Bacteriol. , vol.195 , pp. 4826-4835
    • Weaver, E.A.1    Wyckoff, E.E.2    Mey, A.R.3    Morrison, R.4    Payne, S.M.5
  • 41
    • 79551471348 scopus 로고    scopus 로고
    • Construction and characterization of a lactose-inducible promoter system for controlled gene expression in Clostridium perfringens
    • Hartman AH, Liu H, Melville SB. 2011. Construction and characterization of a lactose-inducible promoter system for controlled gene expression in Clostridium perfringens. Appl. Environ. Microbiol. 77:471-478. http: //dx.doi.org/10.1128/AEM.01536-10.
    • (2011) Appl. Environ. Microbiol. , vol.77 , pp. 471-478
    • Hartman, A.H.1    Liu, H.2    Melville, S.B.3
  • 42
    • 83255187995 scopus 로고    scopus 로고
    • Development and characterization of a xylose-inducible gene expression system for Clostridium perfringens
    • Nariya H, Miyata S, Kuwahara T, Okabe A. 2011. Development and characterization of a xylose-inducible gene expression system for Clostridium perfringens. Appl. Environ. Microbiol. 77:8439-8441. http://dx.doi.org/10.1128/AEM.05668-11.
    • (2011) Appl. Environ. Microbiol. , vol.77 , pp. 8439-8441
    • Nariya, H.1    Miyata, S.2    Kuwahara, T.3    Okabe, A.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.