Prevalence of bloodstream pathogens is higher in neonatal encephalopathy cases vs. controls using a novel panel of real-time PCR assays

PLoS ONE

Volumn 9, Issue 5, 2014, Pages

  Tann, Cally J ^a,e   Nkurunziza, Peter ^b   Nakakeeto, Margaret ^e   Oweka, James ^b   Kurinczuk, Jennifer J ^f   Were, Jackson ^b   Nyombi, Natasha ^e   Hughes, Peter ^c   Willey, Barbara A ^a   Elliott, Alison M ^a,b   Robertson, Nicola J ^c   Klein, Nigel ^d,g   Harris, Kathryn A ^d,g  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^b MEDICAL RESEARCH COUNCIL   (United Kingdom)

^c ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^d UNIVERSITY COLLEGE LONDON   (United Kingdom)

^e Mulago National Hospital   (Uganda)

^f UNIVERSITY OF OXFORD   (United Kingdom)

^g GREAT ORMOND STREET HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTEREMIA; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BLOOD CULTURE; BLOODSTREAM INFECTION; BRAIN DISEASE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; CYTOMEGALOVIRUS INFECTION; ENTEROBACTERIACEAE; FEMALE; HERPES SIMPLEX; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALARIA FALCIPARUM; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; NEONATAL ENCEPHALOPATHY; NEWBORN; NEWBORN DISEASE; NEWBORN INFECTION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS GROUP A; VIRUS DETECTION; VIRUS IDENTIFICATION; BLOOD; BRAIN DISEASES; CASE CONTROL STUDY; COMORBIDITY; CYTOMEGALOVIRUS; DNA SEQUENCE; EPIDEMIOLOGY; GENETICS; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMODIUM FALCIPARUM; PREVALENCE; PROCEDURES; SIMPLEXVIRUS; SPECIES DIFFERENCE; UGANDA;

PRIMER DNA;

BACTEREMIA; BASE SEQUENCE; BRAIN DISEASES; CASE-CONTROL STUDIES; COMORBIDITY; CYTOMEGALOVIRUS; DNA PRIMERS; ENTEROBACTERIACEAE; HUMANS; INFANT, NEWBORN; MOLECULAR SEQUENCE DATA; PLASMODIUM FALCIPARUM; PREVALENCE; REAL-TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SIMPLEXVIRUS; SPECIES SPECIFICITY; UGANDA;

EID: 84901352168     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0097259     Document Type: Article

References (15)

1. Eklind S, Mallard C, Leverin AL, Gilland E, Biomgren K, et al. (2001) Bacterial endotoxin sensitizes the immature brain to hypoxic-ischaemic injury. Eur J Neurosci 13: 1101-1106. (Pubitemid 32275747)
2. Mallard C, Welin AK, Peebles D, Hagberg H, Kjellmer I (2003) White matter injury following systemic endotoxemia or asphyxia in the fetal sheep. Neurochem Res 28: 215-223. (Pubitemid 36207346)
3. Eklind S, Mallard C, Arvidsson P, Hagberg H (2005) Lipopolysaccharide induces both a primary and a secondary phase of sensitization in the developing rat brain. Pediatric Research 58: 112-116. (Pubitemid 40967439)
4. Mallard C (2012) Innate immune regulation by Toll-like receptors in the brain. ISRN Neurology 2012: 19.
5. Stridh L, Mottahedin A, Johansson ME, Valdez RC, Northington F, et al. (2013) Toll-like receptor-3 increases the vulnerability of the neonatal brain to hypoxia-ischaemia. Journal of Neuroscience 33: 12041-12051.
6. Peebles D (2007) Intrauterine infection and perinatal brain injury. 1-7 p.
7. Pammi M, Flores A, Leeflang M, Versalovic J (2011) Molecular assays in the diagnosis of neonatal sepsis: A systematic review and meta-analysis. Pediatrics 128: e973-e985.
8. Harris KA, Hartley JC (2003) Development of broad-range 16 s rDNA PCR for use in the routine diagnostic clinical microbiology service Journal of Medical Microbiology 5: 685-691. (Pubitemid 36981477)
9. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Kaczmarski EB, et al. (2000) Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. Journal of Clinical Microbiology 38: 1747-1752. (Pubitemid 30253908)
10. Harris KA, Turner P, Green EA, Hartley JC (2008) A duplex real-time PCR assay for the detection, and determination of penicillin susceptibility, of Streptococcus pneumoniae in clinical samples Journal of Clinical Microbiology 46: 2751-2758.
11. Thompson CM, Puterman AS, Linley LL, Hann FM, van der Elst CW, et al. (1997) The value of a scoring system for hypoxic ischaemic encephalopathy in predicting neurodevelopmental outcome. Acta Paediatr 86: 757-761. (Pubitemid 27327126)
12. Edmond K, Zaidi A (2010) New approaches to preventing, diagnosing, and treating neonatal sepsis. Plos Medicine 7: e1000213.
13. McAdams R, Juul S (2012) The role of cytokines and inflammatory cells in perinatal brain injury. Neurology Research International: 15 pages.
14. Manicklal S, Emery VC, Lazzarotto T, Boppana SB, Gupta RK (2013) The "Silent" Global Burden of Congenital Cytomegalovirus. Clinical Microbiology Reviews 26: 86-102.
15. van der Sande MAB, Kaye S, Miles DJC, Waight P, Jefferies DJ, et al. (2007) Risk factors for and Clinical Outcome of Congenital Cytomegalovirus Infection in a Peri-Urban West-African Birth Cohort. Plos ONE 2: e492.