A novel approach for purification and selective capture of membrane vesicles of the periodontopathic bacterium, Porphyromonas gingivalis: Membrane vesicles bind to magnetic beads coated with epoxy groups in a noncovalent, species-specific manner

PLoS ONE

Volumn 9, Issue 5, 2014, Pages

  Nakao, Ryoma ^a   Kikushima, Kenji ^b   Higuchi, Hideo ^b   Obana, Nozomu ^c   Nomura, Nobuhiko ^c   Bai, Dongying ^d   Ohnishi, Makoto ^a   Senpuku, Hidenobu ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c UNIVERSITY OF TSUKUBA   (Japan)

^d TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EPOXIDE; NANOPARTICLE; SUPERPARAMAGNETIC BEAD; UNCLASSIFIED DRUG; BACTERIAL PROTEIN;

ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; BINDING ASSAY; BINDING COMPETITION; CANCER CELL CULTURE; CELL DETACHMENT; CELL ISOLATION; CELL KINETICS; CONTROLLED STUDY; DENATURATION; DISSOCIATION; ELECTRON MICROSCOPY; FIMBRIA; FRACTIONATION; HUMAN; HUMAN CELL; MATERIAL COATING; MEMBRANE VESICLE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PORPHYROMONAS GINGIVALIS; PURIFICATION; CELL MEMBRANE; CULTURE TECHNIQUE; MAGNETISM; MASS SPECTROMETRY; METABOLISM; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MICROSCOPY; PERIODONTITIS; PROCEDURES; SPECIES DIFFERENCE; TUMOR CELL LINE;

BACTERIAL PROTEINS; BINDING, COMPETITIVE; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL MEMBRANE; FIMBRIAE, BACTERIAL; HUMANS; MAGNETICS; MICROBIOLOGICAL TECHNIQUES; MICROSCOPY; MICROSCOPY, ELECTRON; PERIODONTITIS; PORPHYROMONAS GINGIVALIS; SPECIES SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84901296074     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0095137     Document Type: Article

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