Multi-probe Real-Time PCR Identification of Four Common Candida Species in Blood Culture Broth

Mycopathologia

Volumn 177, Issue 5-6, 2014, Pages 251-261

  Foongladda, Suporn ^a   Mongkol, Nanthanida ^a   Petlum, Pornphan ^a   Chayakulkeeree, Methee ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Blood; Candida; Candidemia; Identification; Probes; Real time PCR

Indexed keywords

CULTURE MEDIUM; FUNGAL DNA; PRIMER DNA;

BLOOD; CANDIDA; CANDIDA ALBICANS; CANDIDA GLABRATA; CANDIDA TROPICALIS; CANDIDIASIS; CULTURE MEDIUM; EVALUATION STUDY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION;

BLOOD; CANDIDA; CANDIDA ALBICANS; CANDIDA GLABRATA; CANDIDA TROPICALIS; CANDIDIASIS; CULTURE MEDIA; DNA PRIMERS; DNA, FUNGAL; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84901036011     PISSN: 0301486X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11046-014-9743-7     Document Type: Article

References (23)

1. Mullen CA, El-Baki HA, Samir H, Tarrand JJ, Rolston KV. Non-albicans Candida is the most common cause of candidemia in pediatric cancer patients. Support Care Cancer. 2003;11: 321-5.
2. Fidel PL Jr, Vazquez JA, Sobel JD. Candida glabrata: review of epidemiology, pathogenesis, and clinical disease with comparison to C. albicans. Clin Microbiol Rev. 1999;12: 80-96.
3. Krcmery V, Barnes AJ. Non-albicans Candida spp. causing fungaemia: pathogenicity and antifungal resistance. J Hosp Infect. 2002;50: 243-60.
4. Trofa D, Gacser A, Nosanchuk JD. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev. 2008;21: 606-25.
5. Pfaller MA, Diekema DJ. Progress in antifungal susceptibility testing of Candida spp. by use of Clinical and Laboratory Standards Institute broth microdilution methods, 2010 to 2012. J Clin Microbiol. 2010;2012(50): 2846-56.
6. Freydiere AM, Odds FC. Commercial kits for yeast identification: concerns for standardisation. Eur J Clin Microbiol Infect Dis. 2001;20: 366-7.
7. Freydiere AM, Guinet R, Boiron P. Yeast identification in the clinical microbiology laboratory: phenotypical methods. Med Mycol. 2001;39: 9-33.
8. Ellepola AN, Morrison CJ. Laboratory diagnosis of invasive candidiasis. J Microbiol. 2005;43: 65-84.
9. Elie CM, Lott TJ, Reiss E, Morrison CJ. Rapid identification of Candida species with species-specific DNA probes. J Clin Microbiol. 1998;36: 3260-5.
10. Bougnoux M, Dupont C, Mateo J, Saulnier P, Faivre V, Payen D, Nicolas-Chanoine M. Serum is more suitable than whole blood for diagnosis of systemic candidiasis by nested PCR. J Clin Microbiol. 1999;37: 925-30.
11. Guiver M, Levi K, Oppenheim BA. Rapid identification of Candida species by TaqMan PCR. J Clin Pathol. 2001;54: 362-6.
12. Maaroufi Y, Heymans C, De Bruyne JM, Duchateau V, Rodriguez-Villalobos H, Aoun M, Crokaert F. Rapid detection of Candida albicans in clinical blood samples by using a TaqMan-based PCR assay. J Clin Microbiol. 2003;41: 3293-8.
13. Schabereiter-Gurtner C, Selitsch B, Rotter ML, Hirschl AM, Willinger B. Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens. J Clin Microbiol. 2007;45: 906-14.
14. Fricke S, Fricke C, Schimmelpfennig C, Oelkrug C, Schönfelder U, Blatz R, Zilch C, Faber S, Hilger N, Ruhnke M, Rodloff AC. A real-time PCR assay for the differentiation of Candida species. J Appl Microbiol. 2010;109: 1150-8.
15. Selvarangan R, Bui U, Limaye AP, Cookson BT. Rapid identification of commonly encountered Candida species directly from blood culture bottles. J Clin Microbiol. 2003;41: 5660-4.
16. Gherna M, Merz WG. Identification of Candida albicans and Candida glabrata within 1. 5 hours directly from positive blood culture bottles with a shortened peptide nucleic acid fluorescence in situ hybridization protocol. J Clin Microbiol. 2009;47: 247-8.
17. Lagacé-Wiens PR, Adam HJ, Karlowsky JA, Nichol KA, Pang PF, Guenther J, Webb AA, Miller C, Alfa MJ. Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time. J Clin Microbiol. 2012;50: 3324-8.
18. Foongladda S, Pholwat S, Eampokalap B, Kiratisin P, Sutthent R. Multi-probe real-time PCR identification of common mycobacterium species in blood culture broth. J Mol Diagn. 2009;11: 42-8.
19. Quiles-Melero I, García-Rodriguez J, Romero-Gómez MP, Gómez-Sánchez P, Mingorance J. Rapid identification of yeasts from positive blood culture bottles by pyrosequencing. Eur J Clin Microbiol Infect Dis. 2011;30: 21-4.
20. Fenn JP, Billetdeaux E, Segal H, Skodack-Jones L, Padilla PE, Bale M, Carroll K. Comparison of four methodologies for rapid and cost-effective identification of Candida glabrata. J Clin Microbiol. 1999;37: 3387-9.
21. Vallini G, Frassinetti S, Scorzetti G. Candida aquaetextoris sp. nov., a new species of yeast occurring in sludge from a textile industry wastewater treatment plant in Tuscany, Italy. Int J Syst Bacteriol. 1997;47: 336-40.
22. Suh SO, Blackwell M. Three new beetle-associated yeast species in the Pichia guilliermondii clade. FEMS Yeast Res. 2004;5: 87-95.
23. Suh SO, McHugh JV, Pollock DD, Blackwell M. The beetle gut: a hyperdiverse source of novel yeasts. Mycol Res. 2005;109: 261-5.