Characterization of ubiquitin-activating enzyme Uba1 in the nucleus by its mammalian temperature-sensitive mutant

PLoS ONE

Volumn 9, Issue 5, 2014, Pages

  Sugaya, Kimihiko ^a   Ishihara, Yoshie ^a   Inoue, Sonoe ^a   Tsuji, Hideo ^a  

^a NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GEMININ; GREEN FLUORESCENT PROTEIN; ISOLEUCINE; METHIONINE; UBA1 PROTEIN; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANTIBODY LABELING; ARTICLE; CELL CYCLE; CELL ISOLATION; CELL LABELING; CELL LEVEL; CELL LINE; CELL MUTANT; CELL NUCLEUS; CONTROLLED STUDY; ENZYME ANALYSIS; GENE SEQUENCE; HAMSTER; IMMUNOBLOTTING; INCUBATION TEMPERATURE; MAMMAL; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; TEMPERATURE SENSITIVE MUTANT; TSTM3 CELL LINE; UBA1 GENE; UBIQUITINATION; WESTERN BLOTTING; WILD TYPE; ANIMAL; CHO CELL LINE; CRICETULUS; GENETICS; METABOLISM; MUTATION; TEMPERATURE;

ANIMALS; CELL NUCLEUS; CHO CELLS; CRICETINAE; CRICETULUS; MUTATION; TEMPERATURE; UBIQUITIN-ACTIVATING ENZYMES;

EID: 84900551136     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0096666     Document Type: Article

References (37)

1. Hershko A, Ciechanover A (1992) The ubiquitin system for protein degradation. Annu Rev Biochem 61: 761-807.
2. Pickart CM (2004) Back to the future with ubiquitin. Cell 116: 181-190.
3. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132: 487-498. (Pubitemid 351191996)
4. Schulman BA, Harper JW (2009) Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways. Nat Rev Mol Cell Biol 10: 319-331.
5. Handley-Gearhart PM, Stephen AG, Trausch-Azar JS, Ciechanover A, Schwartz AL (1994) Human ubiquitin-activating enzyme, E1. Indication of potential nuclear and cytoplasmic subpopulations using epitope-tagged cDNA constructs. J Biol Chem 269: 33171-33178.
6. Jin J, Li X, Gygi SP, Harper JW (2007) Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 447: 1135-1138. (Pubitemid 47014428)
7. Chiu YH, Sun Q, Chen ZJ (2007) E1-L2 activates both ubiquitin and FAT10. Mol Cell 27: 1014-1023. (Pubitemid 47488190)
8. Tsuji H, Matsudo Y, Tsuji S, Hanaoka F, Hyodo M, et al. (1990) Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature. Somatic Cell Mol Genet 16: 461-476. (Pubitemid 20376552)
9. Sugaya K, Sasanuma S, Nohata J, Kimura T, Hongo E, et al. (1997) Cloning and sequencing for the largest subunit of Chinese hamster RNA polymerase II gene: identification of a mutation related to abnormal induction of sister chromatid exchanges. Gene 194: 267-272. (Pubitemid 27348307)
10. Sugaya K, Ajimura M, Tsuji H, Morimyo M, Mita K (1998) Alteration of the largest subunit of RNA polymerase II and its effect on chromosome stability in Schizosaccharomyces pombe. Mol Gen Genet 258: 279-287.
11. Sugaya K, Hongo E, Tsuji H (2005) A temperature-sensitive mutation in the WD repeat-containing protein Smu1 is related to maintenance of chromosome integrity. Exp Cell Res 306: 242-251. (Pubitemid 40692787)
12. Sugaya K, Hongo E, Ishihara Y, Tsuji H (2006) The conserved role of Smu1 in splicing is characterized in its mammalian temperature-sensitive mutant. J Cell Sci 119: 4944-4951. (Pubitemid 46017807)
13. Mita S, Yasuda H, Marunouchi T, Ishiko S, Yamada M (1980) A temperature-sensitive mutant of cultured mouse cells defective in chromosome condensation. Exp Cell Res 126: 407-416. (Pubitemid 10041175)
14. Hyodo M, Suzuki K (1982) A temperature-sensitive mutant isolated from mouse FM3A cells defective in DNA replication at a non-permissive temperature. Exp Cell Res 137: 31-38. (Pubitemid 12146136)
15. Marcus M, Hirschberg J (1982) Cell cycle analysis of temperature- sensitive mutants by premature chromosome condensation. In: Rao RN, Johnson RT, Sperling K, editors. Premature Chromosome Condensation in Basic, Clinical, and Mutation Research. New York: Academic Press. pp. 173-194.
16. Zeng GC, Donegan J, Ozer HL, Hand R (1984) Characterization of a ts mutant of BALB/3T3 cells and correction of the defect by in vitro addition of extracts from wild-type cells. Mol Cell Biol 4: 1815-1822. (Pubitemid 14008292)
17. Ohtsubo M, Nishimoto T (1988) The gene coding a ubiquitin-activating enzyme may locate on X chromosome. Biochem Biophys Res Commun 153: 1173-1178.
18. Ayusawa D, Kaneda S, Itoh Y, Yasuda H, Murakami Y, et al. (1992) Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1. Cell Struct Funct 17: 113-122.
19. Mori M, Eki T, Takahashi-Kudo M, Hanaoka F, Ui M, et al. (1993) Characterization of DNA synthesis at a restrictive temperature in the temperature-sensitive mutants, tsFT5 cells, that belong to the complementation group of ts85 cells containing a thermolabile ubiquitin-activating enzyme E1. Involvement of the ubiquitin-conjugating system in DNA replication. J Biol Chem 268: 16803-16809. (Pubitemid 23229997)
20. Ramser J, Ahearn ME, Lenski C, Yariz KO, Hellebrand H, et al. (2008) Rare missense and synonymous variants in UBE1 are associated with X-linked infantile spinal muscular atrophy. Am J Hum Genet 82: 188-193. (Pubitemid 351726086)
21. Lao T, Chen S, Sang N (2012) Two mutations impair the stability and function of ubiquitin-activating enzyme (E1). J Cell Physiol 227: 1561-1568.
22. Kulka RG, Raboy B, Schuster R, Parag HA, Diamond G, et al. (1988) A Chinese hamster cell cycle mutant arrested at G2 phase has a temperature-sensitive ubiquitin-activating enzyme, E1. J Biol Chem 263: 15726-15731.
23. Deveraux Q, Wells R, Rechsteiner M (1990) Ubiquitin metabolism in ts85 cells, a mouse carcinoma line that contains a thermolabile ubiquitin activating enzyme. J Biol Chem 265: 6323-6329.
24. Salvat C, Acquaviva C, Scheffner M, Robbins I, Piechaczyk M, et al. (2000) Molecular characterization of the thermosensitive E1 ubiquitin-activating enzyme cell mutant A31N-ts20. Requirements upon different levels of E1 for the ubiquitination/degradation of the various protein substrates in vivo. Eur J Biochem 267: 3712-3722. (Pubitemid 30423033)
25. Yang U, Yang HY, Kim JS, Lee TH (2012) The functional role of UBA1 cysteine-278 in ubiquitination. Biochem Biophys Res Commun 427: 587-592.
26. Stephen AG, Trausch-Azar JS, Handley-Gearhart PM, Ciechanover A, Schwartz AL (1997) Identification of a region within the ubiquitin-activating enzyme required for nuclear targeting and phosphorylation. J Biol Chem 272: 10895-10903. (Pubitemid 27181107)
27. Grenfell SJ, Trausch-Azar JS, Handley-Gearhart PM, Ciechanover A, Schwartz AL (1994) Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent. Biochem J 300: 701-708. (Pubitemid 24180583)
28. Stephen AG, Trausch-Azar JS, Ciechanover A, Schwartz AL (1996) The ubiquitin-activating enzyme E1 is phosphorylated and localized to the nucleus in a cell cycle-dependent manner. J Biol Chem 271: 15608-15614. (Pubitemid 26225335)
29. Chowdary DR, Dermody JJ, Jha KK, Ozer HL (1994) Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway. Mol Cell Biol 14: 1997-2003. (Pubitemid 24074596)
30. Moudry P, Lukas C, Macurek L, Hanzlikova H, Hodny Z, et al. (2012) Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage. Cell Cycle 11: 1573-1582.
31. Blow JJ, Dutta A (2005) Preventing re-replication of chromosomal DNA. Nat Rev Mol Cell Biol 6: 476-486. (Pubitemid 40780559)
32. Klotz-Noack K, McIntosh D, Schurch N, Pratt N, Blow JJ (2012) Re-replication induced by geminin depletion occurs from G2 and is enhanced by checkpoint activation. J Cell Sci 125: 2436-2445.
33. Kisielewska J, Blow JJ (2012) Dynamic interactions of high Cdt1 and geminin levels regulate S phase in early Xenopus embryos. Development 139: 63-74.
34. Sugaya K, Ishihara Y, Sugaya K (2011) Enlargement of speckles of SF2/ASF due to loss of function of Smu1 is characterized in the mammalian temperature-sensitive mutant. RNA Biol 8: 488-495.
35. Kimura H, Tao Y, Roeder RG, Cook PR (1999) Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure. Mol Cell Biol 19: 5383-5392. (Pubitemid 29339897)
36. Hongo E, Ishihara Y, Sugaya K, Sugaya K (2008) Characterization of cells expressing RNA polymerase II tagged with green fluorescent protein: effect of ionizing irradiation on RNA synthesis. Int J Radiat Biol 84: 778-787.
37. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680. (Pubitemid 24354800)