Multiplex viral polymerase chain reaction testing using the FilmArray device compared with direct fluorescent antibody testing

Lab Medicine

Volumn 45, Issue 1, 2014, Pages 62-64

  Wang, Jason ^a   Simons, Debra B ^b   Adams, James L ^b   Jerris, Robert C ^a,b   Rogers, Beverly Barton ^a,b  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CHILDREN'S HEALTHCARE OF ATLANTA   (United States)

Author keywords

DFA test; FilmArray; Lean; PCR; Rapid antigen testing; Respiratory panel

Indexed keywords

ANALYTICAL EQUIPMENT; ARTICLE; CONTROLLED STUDY; FILMARRAY RESPIRATORY PANEL; FLUORESCENT ANTIBODY TECHNIQUE; HANDS ON TIME; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; OPERATOR; RESPIRATORY VIRUS; TIME; TURNAROUND TIME; HUMAN; POLYMERASE CHAIN REACTION; RESPIRATORY TRACT INFECTION; VIROLOGY;

BACTERIA (MICROORGANISMS);

BACTERIAL DNA; VIRUS DNA;

DNA, BACTERIAL; DNA, VIRAL; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; HUMANS; POLYMERASE CHAIN REACTION; RESPIRATORY TRACT INFECTIONS;

EID: 84900542597     PISSN: 00075027     EISSN: 19437730     Source Type: Journal    
DOI: 10.1309/LMOMIXQ6N4JAPDX1     Document Type: Article

References (7)

1. th Annual Clinical Virology Symposium. Daytona Beach, Florida2011.
2. Kuypers J, Wright N, Ferrenberg J, et al. Comparison of real-time PCR assays with fluorescent-antibody assays for diagnosis of respiratory virus infections in children. J Clin Microbiol. 2006;44(7):2382-2388.
3. Freymuth F, Vabret A, Cuvillon-Nimal D, et al. Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness. J Med Virol. 2006;78(11):1498-1504.
4. Miernyk K, Bulkow L, DeByle C, et al. Performance of a rapid antigen test (Binax NOW(R) RSV) for diagnosis of respiratory syncytial virus compared with real-time polymerase chain reaction in a pediatric population. J Clin Virol. 2011;50(3):240-243.
5. Pierce VM, Elkan M, Leet M, McGowan KL, Hodinka RL. Comparison of the Idaho Technology FilmArray system to real-time PCR for detection of respiratory pathogens in children. J Clin Microbiol. 2012;50(2):364-371.
6. nd ed. New York: Productivity Press/Taylor & Francis Group; 2010.
7. Poritz MA, Blaschke AJ, Byington CL, et al. FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection. PLoS One. 2011;6(10):e26047.