Arabidopsis ABA receptor RCAR1/PYL9 interacts with an R2R3-type MYB transcription factor, AtMYB44

International Journal of Molecular Sciences

Volumn 15, Issue 5, 2014, Pages 8473-8490

  Li, Dekuan ^a   Li, Ying ^a   Zhang, Liang ^a   Wang, Xiaoyu ^a   Zhao, Zhe ^a   Tao, Zhiwen ^a   Wang, Jianmei ^a   Wang, Jin ^b   Lin, Min ^b   Li, Xufeng ^a   Yang, Yi ^a  

^a SICHUAN UNIVERSITY   (China)

^b AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

Author keywords

ABA; ABI1; AtMYB44; RCAR1 PYL9; Receptor; Signaling

Indexed keywords

ABA RECEPTOR; ABSCISIC ACID; COMPLEMENTARY DNA; GLUTATHIONE TRANSFERASE; HORMONE RECEPTOR; JASMONIC ACID; PHOSPHOPROTEIN PHOSPHATASE 2; PHOSPHOPROTEIN PHOSPHATASE 2C; PHYTOHORMONE; PROTEIN KINASE; PROTEIN KINASE SNRK2; PROTEIN MYB; REACTIVE OXYGEN METABOLITE; SALICYLIC ACID; SNF1 PROTEIN KINASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR MYB 44; UNCLASSIFIED DRUG; ABI1 PROTEIN, ARABIDOPSIS; ARABIDOPSIS PROTEIN; CARRIER PROTEIN; MYB44 PROTEIN, ARABIDOPSIS; PHOSPHOPROTEIN PHOSPHATASE; RCAR1 PROTEIN, ARABIDOPSIS;

ARABIDOPSIS; ARTICLE; BIOMOLECULAR FLUORESCENCE COMPLEMENTATION ASSAY; ENZYME ASSAY; FLUORESCENCE ANALYSIS; GENE; GENE EXPRESSION; GENETIC ANALYSIS; GERMINATION; GST PULL DOWN ASSAY; NUCLEOTIDE SEQUENCE; PLANT GROWTH; RAB18 GENE; REAL TIME POLYMERASE CHAIN REACTION; REGULATORY MECHANISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; UPREGULATION; WESTERN BLOTTING; CHEMISTRY; DRUG EFFECTS; METABOLISM; PROTEIN DOMAIN; TWO HYBRID SYSTEM;

ABSCISIC ACID; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CARRIER PROTEINS; PHOSPHOPROTEIN PHOSPHATASES; PROTEIN INTERACTION DOMAINS AND MOTIFS; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 84900513857     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms15058473     Document Type: Article

References (43)

1. Finkelstein, R.R.; Gampala, S.S.; Rock, C.D. Abscisic acid signaling in seeds and seedlings. Plant Cell 2002, 14, S15-S45.
2. Zheng, X.; Chen, B.; Lu, G.; Han, B. Overexpression of a NAC transcription factor enhances rice drought and salt tolerance. Biochem. Biophys. Res. Commun. 2009, 379, 985-989.
3. Cutler, S.R.; Rodriguez, P.L.; Finkelstein, R.R.; Abrams, S.R. Abscisic acid: Emergence of a core signaling network. Annu. Rev. Plant Biol. 2010, 61, 651-679.
4. Hubbard, K.E.; Nishimura, N.; Hitomi, K.; Getzoff, E.D.; Schroeder, J.I. Early abscisic acid signal transduction mechanisms: Newly discovered components and newly emerging questions. Genes Dev. 2010, 24, 1695-1708.
5. Ma, Y.; Szostkiewicz, I.; Korte, A.; Moes, D.; Yang, Y.; Christmann, A.; Grill, E. Regulators of PP2C phosphatase activity function as abscisic acid sensors. Science 2009, 324, 1064-1068.
6. Park, S.-Y.; Fung, P.; Nishimura, N.; Jensen, D.R.; Fujii, H.; Zhao, Y.; Lumba, S.; Santiago, J.; Rodrigues, A.; Chow, T.-F.F. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 2009, 324, 1068-1071.
7. Santiago, J.; Rodrigues, A.; Saez, A.; Rubio, S.; Antoni, R.; Dupeux, F.; Park, S.Y.; Márquez, J.A.; Cutler, S.R.; Rodriguez, P.L. Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs. Plant J. 2009, 60, 575-588.
8. Saavedra, X.; Modrego, A.; Rodríguez, D.; González-García, M.P.; Sanz, L.; Nicolás, G.; Lorenzo, O. The nuclear interactor PYL8/RCAR3 of Fagus sylvatica FsPP2C1 is a positive regulator of abscisic acid signaling in seeds and stress. Plant Physiol. 2010, 152, 133-150.
9. Fuchs, S.; Grill, E.; Meskiene, I.; Schweighofer, A. Type 2C protein phosphatases in plants. FEBS J. 2012, 280, 681-693.
10. Yoshida, R.; Umezawa, T.; Mizoguchi, T.; Takahashi, S.; Takahashi, F.; Shinozaki, K. The regulatory domain of SRK2E/OST1/SnRK2. 6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis. J. Biol. Chem. 2006, 281, 5310-5318.
11. Fujii, H.; Verslues, P.E.; Zhu, J.-K. Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis. Plant Cell 2007, 19, 485-494.
12. Nakashima, K.; Fujita, Y.; Kanamori, N.; Katagiri, T.; Umezawa, T.; Kidokoro, S.; Maruyama, K.; Yoshida, T.; Ishiyama, K.; Kobayashi, M. Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2. 2, SRK2E/SnRK2. 6/OST1 and SRK2I/SnRK2. 3, involved in ABA signaling are essential for the control of seed development and dormancy. Plant Cell Physiol. 2009, 50, 1345-1363.
13. Raghavendra, A.S.; Gonugunta, V.K.; Christmann, A.; Grill, E. ABA perception and signalling. Trends Plant Sci. 2010, 15, 395-401.
14. Yamaguchi-Shinozaki, K.; Shinozaki, K. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu. Rev. Plant Biol. 2006, 57, 781-803.
15. Huang, D.; Jaradat, M.R.; Wu, W.; Ambrose, S.J.; Ross, A.R.; Abrams, S.R.; Cutler, A.J. Structural analogs of ABA reveal novel features of ABA perception and signaling in Arabidopsis. Plant J. 2007, 50, 414-428.
16. Jaradat, M.R.; Feurtado, J.A.; Huang, D.; Lu, Y.; Cutler, A.J. Multiple roles of the transcription factor AtMYBR1/AtMYB44 in ABA signaling, stress responses, and leaf senescence. BMC Plant Biol. 2013, 13, 192.
17. Jung, C.; Seo, J.S.; Han, S.W.; Koo, Y.J.; Kim, C.H.; Song, S.I.; Nahm, B.H.; Do Choi, Y.; Cheong, J.-J. Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiol. 2008, 146, 623-635.
18. Persak, H.; Pitzschke, A. Tight interconnection and multi-level control of Arabidopsis MYB44 in MAPK cascade signalling. PLoS One 2013, 8, e57547.
19. Nguyen, X.C.; Hoang, M.H.T.; Kim, H.S.; Lee, K.; Liu, X.-M.; Kim, S.H.; Bahk, S.; Park, H.C.; Chung, W.S. Phosphorylation of the transcriptional regulator MYB44 by mitogen activated protein kinase regulates Arabidopsis seed germination. Biochem. Biophys. Res. Commun. 2012, 423, 703-708.
20. Stracke, R.; Werber, M.; Weisshaar, B. The R2R3-MYB gene family in Arabidopsis thaliana. Curr. Opin. Plant Biol. 2001, 4, 447-456.
21. Lång, V.; Palva, E.T. The expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh. Plant Mol. Biol. 1992, 20, 951-962.
22. Ghelis, T.; Dellis, O.; Jeannette, E.; Bardat, F.; Cornel, D.; Miginiac, E.; Rona, J.-P.; Sotta, B. Abscissic acid specific expression of RAB18 involves activation of anion channels in Arabidopsis thaliana suspension cells. FEBS Lett. 2000, 474, 43-47.
23. Hou, X.; Lee, L.Y.C.; Xia, K.; Yan, Y.; Yu, H. DELLAs modulate jasmonate signaling via competitive binding to JAZs. Dev. Cell 2010, 19, 884-894.
24. Sánchez-Barrena, M.J.; Fujii, H.; Angulo, I.; Martínez-Ripoll, M.; Zhu, J.-K.; Albert, A. The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3. Mol. Cell 2007, 26, 427-435.
25. Gosti, F.; Beaudoin, N.; Serizet, C.; Webb, A.A.; Vartanian, N.; Giraudat, J. ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 1999, 11, 1897-1909.
26. Merlot, S.; Gosti, F.; Guerrier, D.; Vavasseur, A.; Giraudat, J. The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant J. 2001, 25, 295-303.
27. Kranz, H.D.; Denekamp, M.; Greco, R.; Jin, H.; Leyva, A.; Meissner, R.C.; Petroni, K.; Urzainqui, A.; Bevan, M.; Martin, C. Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J. 1998, 16, 263-276.
28. Nishimura, N.; Sarkeshik, A.; Nito, K.; Park, S.Y.; Wang, A.; Carvalho, P.C.; Lee, S.; Caddell, D.F.; Cutler, S.R.; Chory, J. PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis. Plant J. 2010, 61, 290-299.
29. Léon-Kloosterziel, K.M.; Gil, M.A.; Ruijs, G.J.; Jacobsen, S.E.; Olszewski, N.E.; Schwartz, S.H.; Zeevaart, J.A.; Koornneef, M. Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci. Plant J. 1996, 10, 655-661.
30. Shin, R.; Burch, A.Y.; Huppert, K.A.; Tiwari, S.B.; Murphy, A.S.; Guilfoyle, T.J.; Schachtman, D.P. The Arabidopsis transcription factor MYB77 modulates auxin signal transduction. Plant Cell 2007, 19, 2440-2453.
31. Liu, R.; Chen, L.; Jia, Z.; Lü, B.; Shi, H.; Shao, W.; Dong, H. Transcription factor AtMYB44 regulates induced expression of the Ethylene insensitive2 gene in Arabidopsis responding to a harpin protein. Mol. Plant-Microbe Interact. 2011, 24, 377-389.
32. Shim, J.S.; Jung, C.; Lee, S.; Min, K.; Lee, Y.W.; Choi, Y.; Lee, J.S.; Song, J.T.; Kim, J.K.; Choi, Y.D. AtMYB44 regulates WRKY70 expression and modulates antagonistic interaction between salicylic acid and jasmonic acid signaling. Plant J. 2013, 73, 483-495.
33. Persak, H.; Pitzschke, A. Dominant repression by Arabidopsis transcription Factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress. Int. J. Mol. Sci. 2014, 15, 2517-2537.
34. Murashige, T.; Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 1962, 15, 473-497.
35. Jefferson, R.A. Assaying chimeric genes in plants: The Gus gene fusion system. Plant Mol. Biol. Report. 1987, 5, 387-405.
36. Moes, D.; Himmelbach, A.; Korte, A.; Haberer, G.; Grill, E. Nuclear localization of the mutant protein phosphatase abi1 is required for insensitivity towards ABA responses in Arabidopsis. Plant J. 2008, 54, 806-819.
37. Licausi, F.; Weits, D.A.; Pant, B.D.; Scheible, W.R.; Geigenberger, P.; van Dongen, J.T. Hypoxia responsive gene expression is mediated by various subsets of transcription factors and miRNAs that are determined by the actual oxygen availability. New Phytol. 2011, 190, 442-456.
38. Li, D.; Wang, X.; Yuan, D.; Zhang, L.; Jiang, X.; Tao, Z.; Li, Y.; Wang, J.; Li, X.; Yang, Y. Over-expression of ArathEULS3 confers ABA sensitivity and drought tolerance in Arabidopsis. Plant Cell Tissue Organ 2014, doi:10.1007/s11240-014-0453-0.
39. Yang, Y.; Sulpice, R.; Himmelbach, A.; Meinhard, M.; Christmann, A.; Grill, E. Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection. Proc. Natl. Acad. Sci. USA 2006, 103, 6061-6066.
40. Walter, M.; Chaban, C.; Schütze, K.; Batistic, O.; Weckermann, K.; Näke, C.; Blazevic, D.; Grefen, C.; Schumacher, K.; Oecking, C. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J. 2004, 40, 428-438.
41. Yoo, S.-D.; Cho, Y.-H.; Sheen, J. Arabidopsis mesophyll protoplasts: A versatile cell system for transient gene expression analysis. Nat. Protoc. 2007, 2, 1565-1572.
42. Shen, G.; Kuppu, S.; Venkataramani, S.; Wang, J.; Yan, J.; Qiu, X.; Zhang, H. Ankyrin repeat-containing protein 2a is an essential molecular chaperone for peroxisomal membrane-bound Ascorbate peroxidase3 in Arabidopsis. Plant Cell 2010, 22, 811-831.
43. Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 2001, 25, 402-408.