Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies

PLoS ONE

Volumn 9, Issue 5, 2014, Pages

  Zatloukal, Bernhard ^a   Kufferath, Iris ^a   Thueringer, Andrea ^a   Landegren, Ulf ^b   Zatloukal, Kurt ^a   Haybaeck, Johannes ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKERATIN 18; CYTOKERATIN 8; PROTEIN P62; 3,5-DIETHOXYCARBONYL-1,4-DIHYDROCOLLIDINE; GTF2H1 PROTEIN, MOUSE; KERATIN; PYRIDINE DERIVATIVE; TRANSCRIPTION FACTOR;

ACCURACY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL INCLUSION; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FATTY LIVER; IMMUNOFLUORESCENCE MICROSCOPY; IN SITU PROXIMITY LIGATION ASSAY; INTERMETHOD COMPARISON; LIVER CELL; MALE; MALLORY DENK BODY; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; SENSITIVITY AND SPECIFICITY; ANIMAL; CHEMICALLY INDUCED; COMPARATIVE STUDY; CYTOPLASM; DISEASE MODEL; FLUORESCENCE MICROSCOPY; GENETIC PROCEDURES; IMMUNOLOGY; LIVER; MALLORY BODY; METABOLISM; PATHOPHYSIOLOGY; PHYSIOLOGY; PROTEIN PROCESSING; REPRODUCIBILITY; TRANSGENIC MOUSE;

ANIMALS; CYTOPLASM; DISEASE MODELS, ANIMAL; FATTY LIVER; GENETIC TECHNIQUES; HEPATOCYTES; KERATINS; LIVER; MALE; MALLORY BODIES; MICE; MICE, TRANSGENIC; MICROSCOPY, FLUORESCENCE; PROTEIN INTERACTION MAPPING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PYRIDINES; REPRODUCIBILITY OF RESULTS; TRANSCRIPTION FACTORS;

EID: 84900438837     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0096690     Document Type: Article

References (11)

1. Pietraszewska-Bogiel A, Gadella TW (2011) FRET microscopy: from principle to routine technology in cell biology. J Microsc 241: 111-118.
2. Weibrecht I, Leuchowius KJ, Clausson CM, Conze T, Jarvius M, et al. (2010) Proximity ligation assays: a recent addition to the proteomics toolbox. Expert Rev Proteomics 7: 401-409.
3. Aubele M, Spears M, Ludyga N, Braselmann H, Feuchtinger A, et al. (2010) In situ quantification of HER2-protein tyrosine kinase 6 (PTK6) protein-protein complexes in paraffin sections from breast cancer tissues. Br J Cancer 103: 663-667.
4. Zatloukal K, French SW, Stumptner C, Strnad P, Harada M, et al. (2007) From Mallory to Mallory-Denk bodies: what, how and why? Exp Cell Res 313: 2033-2049. (Pubitemid 46842981)
5. Zatloukal K, Stumptner C, Lehner M, Denk H, Baribault H, et al. (2000) Cytokeratin 8 protects from hepatotoxicity, and its ratio to cytokeratin 18 determines the ability of hepatocytes to form Mallory bodies. Am J Pathol 156: 1263-1274. (Pubitemid 30660005)
6. Haybaeck J, Stumptner C, Thueringer A, Kolbe T, Magin TM, et al. (2012) Genetic background effects of keratin 8 and 18 in a DDC-induced hepatotoxicity and Mallory-Denk body formation mouse model. Lab Invest 92: 857-867.
7. Stumptner C, Fuchsbichler A, Heid H, Zatloukal K, Denk H (2002) Mallory body-a disease-associated type of sequestosome. Hepatology 35: 1053-1062. (Pubitemid 34454002)
8. Zatloukal K, Stumptner C, Fuchsbichler A, Heid H, Schnoelzer M, et al. (2002) p62 is a common component of cytoplasmic inclusions in protein aggregation diseases. Am J Pathol 160: 255-263. (Pubitemid 34052286)
9. Magin TM, Vijayaraj P, Leube RE (2007) Structural and regulatory functions of keratins. Exp Cell Res 313: 2021-2032. (Pubitemid 46842973)
10. Holden SJ, Uphoff S, Hohlbein J, Yadin D, Le Reste L, et al. (2010) Defining the limits of single-molecule FRET resolution in TIRF microscopy. Biophys J 99: 3102-3111.
11. Micheva KD, O'Rourke N, Busse B, Smith SJ (2010) Array tomography: high-resolution three-dimensional immunofluorescence. Cold Spring Harb Protoc 2010: pdb to p89.