Ehrlichia and spotted fever group rickettsiae surveillance in Amblyomma americanum in Virginia through use of a novel six-plex real-time PCR assay

Vector-Borne and Zoonotic Diseases

Volumn 14, Issue 5, 2014, Pages 307-316

  Gaines, David N ^a   Operario, Darwin J ^b   Stroup, Suzanne ^b   Stromdahl, Ellen ^c   Wright, Chelsea ^d   Gaff, Holly ^d,e   Broyhill, James ^a   Smith, Joshua ^f   Norris, Douglas E ^g   Henning, Tyler ^g   Lucas, Agape ^h   Houpt, Eric ^b  

^a VIRGINIA DEPARTMENT OF HEALTH   (United States)

^b UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

^c U S ARMY   (United States)

^d OLD DOMINION UNIVERSITY   (United States)

^e UNIVERSITY OF KWAZULU NATAL   (South Africa)

^f Fairfax County Health Department   (United States)

^g JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^h NORTH CAROLINA A AND T STATE UNIVERSITY   (United States)

Author keywords

Ehrlichia; PCR; Real; Rickettsia; Ticks; time RT; Vector borne

Indexed keywords

AMBLYOMMA AMERICANUM; ARTICLE; DISEASE TRANSMISSION; EHRLICHIA; EHRLICHIOSIS; GEOGRAPHIC DISTRIBUTION; NONHUMAN; PREVALENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RICKETTSIA; RICKETTSIOSIS; ROCKY MOUNTAIN SPOTTED FEVER; TICK; UNITED STATES; ANIMAL; DNA SEQUENCE; EPIDEMIOLOGICAL MONITORING; GENETICS; GEOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; IXODIDAE; MICROBIOLOGY; MULTIPLEX POLYMERASE CHAIN REACTION; NYMPH; PARASITE VECTOR; TICK-BORNE DISEASES;

BACTERIAL DNA; PRIMER DNA;

ANIMALS; ARACHNID VECTORS; DNA PRIMERS; DNA, BACTERIAL; EHRLICHIA; EHRLICHIOSIS; EPIDEMIOLOGICAL MONITORING; GEOGRAPHY; HUMANS; IXODIDAE; MULTIPLEX POLYMERASE CHAIN REACTION; NYMPH; REAL-TIME POLYMERASE CHAIN REACTION; RICKETTSIA; ROCKY MOUNTAIN SPOTTED FEVER; SEQUENCE ANALYSIS, DNA; TICK-BORNE DISEASES; VIRGINIA;

EID: 84900431901     PISSN: 15303667     EISSN: 15577759     Source Type: Journal    
DOI: 10.1089/vbz.2013.1509     Document Type: Article

References (32)

1. Ammerman NC, Swanson KI, Anderson JM, Schwartz TR, et al. Spotted-fever group Rickettsia in Dermacentor variabilis, Maryland. Emerg Infect Dis 2004; 10: 1478-1481.
2. Apperson CS, Engber B, Nicholson WL, Mead DG, et al. Tickborne diseases in North Carolina: Is ''Rickettsia amblyommii'' a possible cause of rickettsiosis reported as Rocky Mountain spotted fever? Vector Borne Zoonotic Dis 2008; 8: 597-606.
3. Biggerstaff BJ. PooledInfRate, Version 4.0: a Microsoft-Office Excela Add-in to compute prevalence estimates from pooled samples. Centers for Disease Control and Prevention, Fort Collins, CO, USA; 2009.
4. Chapman AS, Murphy SM, Demma LJ, Holman RC, et al. Rocky Mountain spotted fever in the United States, 1997-2002. Vector Borne Zoonotic Dis 2006; 6: 170-178.
5. Cohen SB, Yabsley MJ, Garrison LE, Freye JD, et al. Rickettsia parkeri in Amblyomma americanum ticks, Tennessee and Georgia, USA. Emerg Infect Dis 2009; 15: 1471-1473.
6. Dahlgren FS, Mandel EJ, Krebs JW, Massung RF, et al. Increasing incidence of Ehrlichia chaffeensis and Anaplasma phagocytophilum in the United States, 2000-2007. Am J Trop Med Hyg 2011; 85: 124-131.
7. Dergousoff SJ, Gajadhar AJ, Chilton NB. Prevalence of Rickettsia species in Canadian populations of Dermacentor andersoni and D. variabilis. Appl Environ Microbiol 2009; 75: 1786-1789.
8. Fornadel CM, Zhang X, Smith JD, Paddock CD, et al. High rates of Rickettsia parkeri infection in Gulf Coast ticks (Amblyomma maculatum) and identification of ''Candidatus Rickettsia andeanae'' from Fairfax County, Virginia. Vector Borne Zoonotic Dis 2011; 11: 1535-1539.
9. Goddard J. Experimental infection of lone star ticks, Amblyomma americanum (L.), with Rickettsia parkeri and exposure of guinea pigs to the agent. J Med Entomol 2003; 40: 686-689.
10. Jiang J, Yarina T, Miller MK, Stromdahl EY, et al. Molecular detection of Rickettsia amblyommii in Amblyomma americanum parasitizing humans. Vector Borne Zoonotic Dis 2010; 10: 329-340.
11. Lackman DB, Parker RR, Gerloff RK. Serological characteristics of a pathogenic rickettsia occurring in Amblyomma maculatum. Public Health Rep 1949; 64: 1342-1349.
12. Lackman DB, Bell EJ, Stoenner HG, Pickens EG. The Rocky Mountain spotted fever group of rickettsias. Health Lab Sci 1965; 2: 135-141.
13. Loftis AD, Massung RF, Levin ML. Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis. Journal of clinical microbiology, 2003; 41: 3870-3872.
14. Loftis AD, Reeves WK, Spurlock JP, Mahan SM, et al. Infection of a goat with a tick-transmitted Ehrlichia from Georgia, U.S.A., that is closely related to Ehrlichia ruminantium. J Vector Ecol 2006; 31: 213-223.
15. Loftis AD, Mixson TR, Stromdahl EY, Yabsley MJ, et al. Geographic distribution and genetic diversity of the Ehrlichia sp. from Panola Mountain in Amblyomma americanum. BMC Infect Dis 2008; 8:54.
16. Mixson TR, Campbell SR, Gill JS, Ginsberg HS, et al. Prevalence of Ehrlichia, Borrelia, and Rickettsial agents in Amblyomma americanum (Acari: Ixodidae) collected from nine states. J Med Entomol 2006; 43: 1261-1268.
17. Moncayo AC, Cohen SB, Fritzen CM, Huang E, et al. Absence of Rickettsia rickettsii and occurrence of other spotted fever group rickettsiae in ticks from Tennessee. Am J Trop Med Hyg 2010; 83: 653-657.
18. Nadolny RM, Wright CL, Hynes WL, Sonenshine DE, et al. Ixodes affinis (Acari: Ixodidae) in southeastern Virginia and implications for the spread of Borrelia burgdorferi, the agent of Lyme disease. J Vector Ecol 2011; 36: 464-467.
19. Openshaw JJ, Swerdlow DL, Krebs JW, Holman RC, et al. Rocky mountain spotted fever in the United States, 2000-2007: Interpreting contemporary increases in incidence. Am J Trop Med Hyg 2010; 83: 174-182.
20. Paddock CD, Yabsley MJ. Ecological havoc, the rise of whitetailed deer, and the emergence of Amblyomma americanumassociated zoonoses in the United States. Curr Top Microbiol Immunol 2007; 315: 289-324.
21. Paddock CD, Sumner JW, Comer JA, Zaki SR, et al. Rickettsia parkeri: A newly recognized cause of spotted fever rickettsiosis in the United States. Clin Infect Dis 2004; 38: 805-811.
22. Paddock CD, Finley RW, Wright CS, Robinson HN, et al. Rickettsia parkeri rickettsiosis and its clinical distinction from Rocky Mountain spotted fever. Clin Infect Dis 2008; 47: 1188-1196.
23. Qurollo BA, Davenport AC, Sherbert BM, Grindem CB, et al. Infection with Panola Mountain Ehrlichia sp. in a dog with atypical lymphocytes and clonal T-cell expansion. J Vet Intern Med 2013; 27: 1251-1255.
24. Reeves WK, Loftis AD, Nicholson WL, Czarkowski AG. The first report of human illness associated with the Panola Mountain Ehrlichia species: A case report. J Med Case Rep 2008; 2:139.
25. Smith MP, Ponnusamy L, Jiang J, Ayyash LA, et al. Bacterial pathogens in ixodid ticks from a Piedmont County in North Carolina: Prevalence of rickettsial organisms. Vector Borne Zoonotic Dis 2010; 10: 939-952.
26. Sonenshine DE, Atwood EL, Lamb JT, Jr. The ecology of ticks transmitting Rocky Mountain spotted fever in a study area in Virginia. Ann Entomol Soc Am 1966; 59: 1234-1262.
27. Stromdahl EY, Hickling GJ. Beyond Lyme: Aetiology of tickborne human diseases with emphasis on the South-Eastern United States. Zoonoses and Public Health 2012; 59: 48-64.
28. Stromdahl EY, Jiang J, Vince M, Richards AL. Infrequency of Rickettsia rickettsii in Dermacentor variabilis removed from humans, with comments on the role of other human-biting ticks associated with spotted fever group Rickettsiae in the United States. Vector Borne Zoonotic Dis 2011; 11: 969-977.
29. Virgina Department of Game and Inland Fisheries. Virginia Deer Management Plan 2006-2015. Virginia Department of Game and Inland Fisheries, 2007.
30. Virginia Department of Health (VDH). Reportable Disease Surveillance in Virginia, 2012.
31. Wright CL, Nadolny RM, Jiang J, Richards AL, et al. Rickettsia parkeri in gulf coast ticks, southeastern Virginia, USA. Emerg Infect Dis 2011; 17: 896-898.
32. Yabsley MJ, Loftis AD, Little SE. Natural and experimental infection of white-tailed deer (Odocoileus virginianus) from the United States with an Ehrlichia sp. closely related to Ehrlichia ruminantium. J Wildl Dis 2008; 44: 381-387.