Detachment and flow cytometric quantification of seagrass-associated bacteria

Journal of Microbiological Methods

Volumn 102, Issue , 2014, Pages 23-25

  Trevathan Tackett, Stacey ^a   Macreadie, Peter ^a   Ralph, Peter ^a   Seymour, Justin ^a  

^a UNIVERSITY OF TECHNOLOGY SYDNEY   (Australia)

Author keywords

Bacterial detachment; Enumeration; Flow cytometry; Microbe; Seagrass; Seagrass associated bacteria

Indexed keywords

POLYSORBATE 80; DETERGENT; POLYSORBATE;

ARTICLE; BACTERIAL CELL; BACTERIUM ADHERENCE; BACTERIUM DETECTION; CONTROLLED STUDY; FLOW CYTOMETRY; NONHUMAN; PLANT PATHOGEN INTERACTION; PRIORITY JOURNAL; PROCESS DEVELOPMENT; QUANTITATIVE ANALYSIS; SEAGRASS; BACTERIA; BACTERIAL LOAD; ISOLATION AND PURIFICATION; MECHANICAL STRESS; MICROBIOLOGY; PROCEDURES; ZOSTERACEAE;

BACTERIA; BACTERIAL LOAD; DETERGENTS; FLOW CYTOMETRY; POLYSORBATES; STRESS, MECHANICAL; ZOSTERACEAE;

EID: 84899950417     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.04.008     Document Type: Article

References (25)

1. Allison S.D., Lu Y., Weihe C., Goulden M.L., Martiny A.C., Treseder K.K., Martiny J.B. Microbial abundance and composition influence litter decomposition response to environmental change. Ecology 2013, 94:714-725.
2. Anesio A.M., Denward C.M.T., Tranvik L.J., Graneli W. Decreased bacterial growth on vascular plant detritus due to photochemical modification. Aquat. Microb. Ecol. 1999, 17:159-165.
3. Blum L.K., Mills A.L. Microbial growth and activity during the initial stages of seagrass decomposition. Mar. Ecol. Prog. Ser. 1991, 70:73-82.
4. Buesing N., Gessner M.O. Comparison of detachment procedures for direct counts of bacteria associated with sediment particles, plant litter and epiphytic biofilms. Aquat. Microb. Ecol. 2002, 27:29-36.
5. Donnelly A., Herbert R. Bacterial interactions in the rhizosphere of seagrass communities in shallow coastal lagoons. J. Appl. Microbiol. 1998, 85:151S-160S.
6. Duarte C.M., Holmer M., Marba N. Plant-microbe interactions in seagrass meadows. Coast. Estuar. Stud. 2005, 60:31-60.
7. Duhamel S., Jacquet S. Flow cytometric analysis of bacteria-and virus-like particles in lake sediments. J. Microbiol. Methods 2006, 64:316-332.
8. García-Martínez M., López-López A., Calleja M.L., Marbà N., Duarte C.M. Bacterial community dynamics in a seagrass (Posidonia oceanica) meadow sediment. Estuar. Coasts 2009, 32:276-286.
9. Gasol J.M., Del Giorgio P.A. Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Sci. Mar. 2000, 64:197-224.
10. Harrison P.G. Detrital processing in seagrass systems: a review of factors affecting decay rates, remineralization and detritivory. Aquat. Bot. 1989, 35:263-288.
11. Holmer M., Andersen F.O., Nielsen S.L., Boschker H.T.S. The importance of mineralization based on sulfate reduction for nutrient regeneration in tropical seagrass sediments. Aquat. Bot. 2001, 71:1-17.
12. Holmer M., Duarte C.M., Boschker H.T.S., Barrón C. Carbon cycling and bacterial carbon sources in pristine and impacted Mediterranean seagrass sediments. Aquat. Microb. Ecol. 2004, 36:227-237.
13. Kenworthy W.J., Currin C.A., Fonseca M.S., Smith G. Production, decomposition, and heterotrophic utilization of the seagrass Halophila decipiens in a submarine canyon. Mar. Ecol. Prog. Ser. 1989, 51:277-290. (Oldendorf).
14. Kuo J., McComb A., Cambridge M. Ultrastructure of the seagrass rhizosphere. New Phytol. 1981, 89:139-143.
15. López N.I., Duarte C.M., Vallespinós F., Romero J., Alcoverro T. Bacterial activity in NW Mediterranean seagrass (Posidonia oceanica) sediments. J. Exp. Mar. Biol. Ecol. 1995, 187:39-49.
16. Louati H., Said O.B., Got P., Soltani A., Mahmoudi E., Cravo-Laureau C., Duran R., Aissa P., Pringault O. Microbial community responses to bioremediation treatments for the mitigation of low-dose anthracene in marine coastal sediments of Bizerte lagoon (Tunisia). Environ. Sci. Pollut. Res. 2013, 20:300-310.
17. Macreadie P.I., Baird M.E., Trevathan-Tackett S.M., Larkum A.W.D., Ralph P.J. Quantifying and modelling the carbon sequestration capacity of seagrass meadows - a critical assessment. Mar. Pollut. Bull 2014, (in press). http://www.sciencedirect.com/science/article/pii/S0025326X13004256. 10.1016/j.marpolbul.2013.07.038.
18. Marie D., Partensky F., Jacquet S., Vaulot D. Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Appl. Environ. Microbiol. 1997, 63:186-193.
19. Mille-Lindblom C., Tranvik L.J. Antagonism between bacteria and fungi on decomposing aquatic plant litter. Microb. Ecol. 2003, 45:173-182.
20. Moran M., Hodson R.E. Bacterial secondary production on vascular plant detritus: relationships to detritus composition and degradation rate. Appl. Environ. Microbiol. 1989, 55:2178-2189.
21. Moriarty D., Iverson R., Pollard P. Exudation of organic carbon by the seagrass Halodule wrightii Aschers. And its effect on bacterial growth in the sediment. J. Exp. Mar. Biol. Ecol. 1986, 96:115-126.
22. Neubauer A.T.A., Pedersen A.G.U., Finster K., Herbert R.A., Donnelly A.P., Viaroli P., Giordani G., De Wit R., Lomstein B.A. Benthic decomposition of Zostera marina roots: a controlled laboratory experiment. J. Exp. Mar. Biol. Ecol. 2004, 313:105-124.
23. Wahbeh M.I., Mahasneh A.M. Heterotrophic bacteria attached to leaves, rhizomes and roots of three seagrass species from Aqaba (Jordan). Aquat. Bot. 1984, 20:87-96.
24. Wahbeh M.I., Mahasneh A.M. Some aspects of decomposition of leaf litter of the seagrass Halophila stipulacea from the gulf of Aqaba (Jordan). Aquat. Bot. 1985, 21:237-244.
25. Williams C.J., Boyer J.N., Jochem F.J. Microbial activity and carbon, nitrogen, and phosphorus content in a subtropical seagrass estuary (Florida Bay): evidence for limited bacterial use of seagrass production. Mar. Biol. 2009, 156:341-353.