Resolving the titer of murine cytomegalovirus by plaque assay using the M2-10B4 cell line and a low viscosity overlay

Virology Journal

Volumn 11, Issue 1, 2014, Pages

  Zurbach, Katherine A ^a   Moghbeli, Toktam ^a   Snyder, Christopher M ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

M2 10B4 cells; Murine cytomegalovirus (MCMV); Plaque assay

Indexed keywords

CARBOXYMETHYLCELLULOSE; CULTURE MEDIUM;

3T3 CELL LINE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BONE MARROW CELL; CELL COUNT; CELL GROWTH; CONTROLLED STUDY; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; CYTOPATHOGENIC EFFECT; FIBROBLAST; M2 10B4 CELL LINE; MOUSE; MURINE CYTOMEGALOVIRUS; NONHUMAN; VIRUS CULTURE; VISCOSITY; ANIMAL; CELL LINE; CHEMISTRY; CULTURE MEDIUM; ISOLATION AND PURIFICATION; MUROMEGALOVIRUS; PROCEDURES; VIRAL PLAQUE ASSAY; VIRUS LOAD;

MAIZE CHLOROTIC MOTTLE VIRUS; MURID HERPESVIRUS 1; MURINAE; MUS;

ANIMALS; CELL LINE; CULTURE MEDIA; MICE; MUROMEGALOVIRUS; VIRAL LOAD; VIRAL PLAQUE ASSAY; VISCOSITY;

EID: 84899937379     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-11-71     Document Type: Article

References (21)

1. A Mouse Model for Cytomegalovirus Infection. Brune W, Hengel H, Koszinowski UH, Current Protocols in Immunology, John Wiley & Sons, Inc, 2001
2. Partial characterization of a novel stromal cell-derived pre-B-cell growth factor active on normal and immortalized pre-B cells. Lemoine FM, Humphries RK, Abraham SD, Krystal G, Eaves CJ, Exp Hematol 1988 16 718 726 3261251
3. Autocrine production of pre-B-cell stimulating activity by a variety of transformed murine pre-B-cell lines. Lemoine FM, Krystal G, Humphries RK, Eaves CJ, Cancer Res 1988 48 6438 6443 3263188
4. Propagation and titration of murine cytomegalovirus in a continuous bone marrow-derived stromal cell line (M2-10B4). Lutarewych MA, Quirk MR, Kringstad BA, Li W, Verfaillie CM, Jordan MC, J Virol Methods 1997 68 193 198 10.1016/S0166-0934(97)00126-2 9389409
5. Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2. Jordan S, Krause J, Prager A, Mitrovic M, Jonjic S, Koszinowski UH, Adler B, J Virol 2011 85 10346 10353 10.1128/JVI.00545-11 21813614
6. Frequent coinfection of cells explains functional in vivo complementation between cytomegalovirus variants in the multiply infected host. Cicin-Sain L, Podlech J, Messerle M, Reddehase MJ, Koszinowski UH, J Virol 2005 79 9492 9502 10.1128/JVI.79.15.9492-9502.2005 16014912
7. Role of murine cytomegalovirus US22 gene family members in replication in macrophages. Menard C, Wagner M, Ruzsics Z, Holak K, Brune W, Campbell AE, Koszinowski UH, J Virol 2003 77 5557 5570 10.1128/JVI.77.10.5557-5570.2003 12719548
8. Interstitial murine cytomegalovirus pneumonia after irradiation: characterization of cells that limit viral replication during established infection of the lungs. Reddehase MJ, Weiland F, Munch K, Jonjic S, Luske A, Koszinowski UH, J Virology 1985 55 264 273 2991554
9. Adverse effects of cytomegalovirus vaccination in mice. Jordan MC, J Clin Invest 1980 65 798 803 10.1172/JCI109730 6244333
10. Evaluation of continuous cell lines in antiviral studies with murine cytomegalovirus. Smee DF, Colletti A, Alaghamandan HA, Allen LB, Arch Virol 1989 107 253 260 10.1007/BF01317921 2554856
11. Activity of 9-(1,3-dihydroxy-2-propoxymethyl)guanine compared with that of acyclovir against human, monkey, and rodent cytomegaloviruses. Freitas VR, Smee DF, Chernow M, Boehme R, Matthews TR, Antimicrob Agents Chemother 1985 28 240 245 10.1128/AAC.28.2.240 3010840
12. The T-cell-independent role of gamma interferon and tumor necrosis factor alpha in macrophage activation during murine cytomegalovirus and herpes simplex virus infections. Heise MT, Virgin HW, J Virol 1995 69 904 909 7815559
13. Spontaneous activation of latent cytomegalovirus from murine spleen explants. Role of lymphocytes and macrophages in release and replication of virus. Jordan MC, Mar VL, J Clin Invest 1982 70 762 768 10.1172/JCI110672 6288769
14. Comparison between human cytomegalovirus pUL97 and murine cytomegalovirus (MCMV) pM97 expressed by MCMV and vaccinia virus: pM97 does not confer ganciclovir sensitivity. Wagner M, Michel D, Schaarschmidt P, Vaida B, Jonjic S, Messerle M, Mertens T, Koszinowski U, J Virol 2000 74 10729 10736 10.1128/JVI.74.22.10729-10736.2000 11044117
15. The murine cytomegalovirus (MCMV) homolog of the HCMV phosphotransferase (UL97(pk)) gene. Rawlinson WD, Zeng F, Farrell HE, Cunningham AL, Scalzo AA, Booth TW, Scott GM, Virology 1997 233 358 363 10.1006/viro.1997.8593 9217058
16. New low-viscosity overlay medium for viral plaque assays. Matrosovich M, Matrosovich T, Garten W, Klenk HD, Virol J 2006 3 63 10.1186/1743-422X-3-63 16945126
17. Systematic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution. Wagner M, Jonjic S, Koszinowski UH, Messerle M, J Virology 1999 73 7056 7060 10400809
18. CD4+ T cell help has an epitope-dependent impact on CD8+ T cell memory inflation during murine cytomegalovirus infection. Snyder CM, Loewendorf A, Bonnett EL, Croft M, Benedict CA, Hill AB, J Immunol 2009 183 3932 3941 10.4049/jimmunol.0900227 19692644
19. Sustained CD8+ T Cell Memory Inflation after Infection with a Single-Cycle Cytomegalovirus. Snyder CM, Cho KS, Bonnett EL, Allan JE, Hill AB, PLoS Pathog 2011 7 1002295 10.1371/journal.ppat.1002295 21998590
20. Requirement for natural killer cell-produced interferon gamma in defense against murine cytomegalovirus infection and enhancement of this defense pathway by interleukin 12 administration. Orange JS, Wang B, Terhorst C, Biron CA, J Exp Med 1995 182 1045 1056 10.1084/jem.182.4.1045 7561678
21. Antiviral T cell response triggers cytomegalovirus hepatitis in mice. Livingston-Rosanoff D, Daley-Bauer LP, Garcia A, McCormick AL, Huang J, Mocarski ES, J Virol 2012 86 12879 12890 10.1128/JVI.01752-12 22993151