A simple heat dissociation method increases significantly the ELISA detection sensitivity of the nonstructural-1 glycoprotein in patients infected with DENV type-4

Journal of Virological Methods

Volumn 204, Issue , 2014, Pages 105-108

  Lima, Monique da Rocha Queiroz ^a   Nogueira, Rita Maria Ribeiro ^a   Filippis, Ana Maria Bispo de ^a   Nunes, Priscila Conrado Guerra ^a   Sousa, Carla Santos de ^a   Silva, Manoela Heringer da ^a   Santos, Flavia Barreto dos ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Dengue diagnosis; DENV 4; Immune complex dissociation; NS1 ELISA; Secondary infections

Indexed keywords

IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; NONSTRUCTURAL PROTEIN 1; ACID; GLYCOPROTEIN; VIRUS ANTIGEN; VIRUS PROTEIN;

ACUTE DISEASE; ANTIGEN ANTIBODY COMPLEX; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DENGUE; DENGUE IGG CAPTURE ELISA KIT; DENGUE IGM CAPTURE ELISA KIT; DENGUE NS1 AG ELISA KIT; DENGUE RAPID TEST; DENGUE VIRUS 4; DIAGNOSTIC TEST ACCURACY STUDY; ELISA KIT; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH TEMPERATURE PROCEDURES; HUMAN; IMMUNOGLOBULIN BLOOD LEVEL; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPING; SIMPLE HEAT DISSOCIATION METHOD; VIRAL DISEASE ELISA KIT; VIRUS DETECTION; VIRUS IDENTIFICATION; VIRUS ISOLATION; VIRUS TYPING; BLOOD; BRAZIL; DENGUE VIRUS; EVALUATION STUDY; HEAT; IMMUNOLOGY; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; PROCEDURES;

ACIDS; ANTIGENS, VIRAL; BRAZIL; DENGUE; DENGUE VIRUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GLYCOPROTEINS; HOT TEMPERATURE; HUMANS; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84899805981     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.02.031     Document Type: Article

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