Molecular and phytochemical analyses to assess genetic stability in alginate-encapsulated microshoots of Ocimum gratissimum L. following in vitro storage

Nucleus (India)

Volumn 57, Issue 1, 2014, Pages 33-43

  Saha, Soumen ^a   Sengupta, Chandan ^a   Ghosh, Parthadeb ^a  

^a UNIVERSITY OF KALYANI   (India)

Author keywords

Encapsulation; GC FID analysis; Genetic analysis; Germplasm conservation; ISSR; Ocimum gratissimum L.; RAPD

Indexed keywords

EID: 84899718735     PISSN: 0029568X     EISSN: 09767975     Source Type: Journal    
DOI: 10.1007/s13237-014-0107-y     Document Type: Article

References (56)

1. Andlib A, Verma RN, Batra A. Synthetic seeds an alternative source for quick regeneration of a zero calorie herb- Stevia rebaudiana bertoni. J Pharm Res. 2011;4: 2007-9.
2. Bekheet SA. A synthetic seed method through encapsulation of in vitro proliferated bulblets of garlic (Allium sativum L.). Arab J Biotech. 2006;9: 415-26.
3. Bekheet SA, Taha HS, Saker MM, Solliman ME. Application of cryopreservation technique for in vitro grown date palm (Phoenix dactylifera L.) cultures. J Appl Sci Res. 2007;3: 859-66.
4. Borner A. Preservation of plant genetic resources in the biotechnology era. Biotechnol J. 2006;1: 1393-404.
5. Clevenger JE. Apparatus for determination of volatile oil. J Am Pharm Assoc. 1928;17: 346-9.
6. Danso KE, Ford-Lloyd BV. Encapsulation of nodal cuttings and shoot tips for storage and exchange of cassava germplasm. Plant Cell Rep. 2003;21: 718-25.
7. Duncan DB. Multiple range and multiple F test. Biometrics. 1955;11: 1-42.
8. Faisal M, Anis M. Regeneration of plants from alginate-encapsulated shoots of Tylophora indica (Burm. f.) Merrill, an endangered medicinal plant. J Hortic Sci Biotechnol. 2007;82: 351-4.
9. Gangopadhyay G, Bandyopadhyay T, Poddar R, Gangopadhyay SB, Mukherjee KK. Encapsulation of pineapple micro shoots in alginate beads for temporary storage. Curr Sci. 2005;88: 972-7.
10. George EF, Hall MA, Deklerk GJ. Plant growth regulators II: cytokinins, their analogues and antagonists. Plant propagation by tissue culture, vol. 3. The Netherlands: Springer; 2008. p. 205-26.
11. Ghosh PD, Chattopadhyay SK, Adhikari S, Saha S, Mondal S, Kader A. Dey ST. Sengupta C. A high throughput DNA extraction method from chemotypically heterogeneous plant species. Protocol Exchange: Bandyopadhyay TK; 2013. doi: 10. 1038/protex. 2013. 018.
12. Ghosh B, Sen S. Plant regeneration from alginate encapsulated somatic embryos of Asparagus cooperi 'Baker'. Plant Cell Rep. 1994;13: 381-5.
13. Gopi C, Nataraja YS, Ponmurugan P. In Vitro Multiplication of Ocimum gratissimum L. through Direct regeneration. Afr J Biotechnol. 2006;5: 723-6.
14. Grzegorczyk I, Wysokinska H. A protocol for synthetic seeds from Salvia officinalis L. shoot tips. Acta Biol Cracov Ser Bot. 2011;53: 80-5.
15. Heywood VH. Flowering plants of the world. USA: Oxford University Press; 1978.
16. Hirai D, Sakai A. Cryopreservation of in vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation vitrification. In: Engelmann F, Takagi H, editors. Cryopreservation of tropical plant germplasm. Current research progress and application. Rome: JIRCAS-IPGRI; 2000. p. 205-11.
17. Jaccard P. Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat. 1908;44: 223-70.
18. Kaviani B. Conservation of plant genetic resources by cryopreservation. Aust J Crop Sci. 2011;5: 778-800.
19. Lata H, Chandra S, Khan IA, ElSohly MA. Propagation through alginate encapsulation of axillary buds of Cannabis sativa L.-an important medicinal plant. Physiol Mol Biol Plants. 2009;15: 79-86.
20. Lata H, Chandra S, Techen N, Khan IA, EISohly MA. Molecular analysis of genetic fidelity in Cannabis sativa L. plants grown from synthetic (encapsulated) seeds following in vitro storage. Biotechnol Lett. 2011;33: 2503-8.
21. Mandal J, Pattnaik S, Chand PK. Alginate encapsulation of axillary buds of Ocimum americanum L. (Hoary Basil), O. basilicum L. (Sweet Basil), O. gratissimum L. (Shrubby Basil), and O. sanctum L. (Sacred Basil). In Vitro Cell Dev Biol Plant. 2000;36: 287-92.
22. Mehrotra S, Khwaja O, Kukreja AK, Rahman L. ISSR and RAPD based evaluation of genetic stability of encapsulated micro shoots of Glycyrrhiza glabra following 6 months of storage. Mol Biotechnol. 2012;52: 262-8.
23. Mishra J, Singh M, Palni LMS, Nandi SK. Assessment of genetic fidelity of encapsulated microshoots of Picrorhiza kurrooa. Plant Cell Tissue Organ Cult. 2011;104: 181-6.
24. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15: 473-97.
25. Nagesh KS, Shanthamma C, Bhagyalakshmi N. Role of polarity in de novo shoot bud initiation from stem discexplants of Curculigo orchioides Gaertn. and its encapsulation and storability. Acta Physiol Plant. 2009;31: 699-704.
26. Naik SK, Chand PK. Nutrient-alginate encapsulation of in vitro nodal segments of Pomegranate (Punica granatum L.) for germplasm distribution and exchange. Sci Hortic. 2006;108: 247-52.
27. Narula A, Kumar S, Srivastava PS. Genetic fidelity of in vitro regenerants, encapsulation of shoot tips and high diosgenin content in Dioscorea bulbifera L., a potential alternative source of diosgenin. Biotechnol Lett. 2007;29: 623-9.
28. Nyende AB, Schittenhelm S, Wagner GM, Greef JM. Production, storability, and regeneration of shoot tips of potato (Solanum tuberosum L.) encapsulated in calcium alginate hollow beads. In Vitro Cell Dev Biol Plant. 2003;39: 540-4.
29. Pattnaik S, Chand PK. Morphogenic responses of the alginate-encapsulated axillary buds from in vitro shoot cultures of six varieties of mulberry. Plant Cell Tissue Organ Cult. 2000;60: 177-85.
30. Powell W, Machray GC, Provan J. Polymorphism revealed by simple sequence repeats. Trends Plant Sci. 1996;1: 215-22.
31. Rao NK. Plant genetic resources: advancing conservation and use through biotechnology. Afr J Biotechnol. 2004;3: 136-45.
32. Ray A, Bhattacharyaa S. Storage and plant regeneration from encapsulated shoot tips of Ruavolfia serpentine-an effective way of conservation and mass propagation. S Afr J Bot. 2008;74: 776-9.
33. Reddy VS, Leelavathi S, Selvapandian A, Raman R, Ferraiolo G, Shukla V, et al. Analysis of chloroplast transformed tobacco plants with cry1Ia5 under rice psbA transcriptional elements reveal high level expression of Bt toxin without imposing yield penalty and stable inheritance of transplastome. Mol Breed. 2002;9: 259-69.
34. Redenbaugh K, Fujii JA, Slade D, Viss PR, Kossler ME. Artificial seeds encapsulated embryos. In: Bajaj YPS, editor. Biotechnology in agriculture and forestry: high technology and micropropagation, vol. 17. Heidelberg: Springer; 1991. p. 395-416.
35. Rohlf FJ. NTSYS-pc: Numerical Taxonomy and multivariate analysis System, Version 2. 1. Setauket: Exeter Publishing; 2002.
36. Roy B, Mandal AB. Development of synthetic seeds involving androgenic embryos and pro-embryos in an elite indica rice. Indian J Biotechnol. 2008;7: 515-9.
37. Saha S, Dey T, Ghosh PD. Micropropagation of Ocimum Kilimandscharicum Guerke (Labiatae). Acta Biol Cracov Ser Bot. 2010;52: 50-8.
38. Saha S, Dhar TN, Sengupta C, Ghosh PD. Biological activities of essential oils and methanol extracts of five Ocimum species against pathogenic bacteria. Czech J Food Sci. 2013;31: 194-202.
39. Saha S, Kader A, Sengupta C, Ghosh PD. In Vitro Propagation of Ocimum gratissimum L. (Lamiaceae) and its evaluation of genetic fidelity using RAPD marker. Am J Plant Sci. 2012;3: 64-74.
40. Sharma S, Shahzad A. Encapsulation technology for short term storage and conservation of a woody climber, Decalepis hamiltonii Wight and Arn. Plant Cell Tissue Organ Cult. 2012;111: 191-8.
41. Siddique I, Anis M. Morphogenic response of the alginate encapsulated nodal segment and antioxidative enzymes analysis during acclimatization of Ocimum basilicum L. J Crop Sci Biotechnol. 2009;12: 233-8.
42. Singh AP, Dwivedi S, Bharti S, Srivastava A, Singh V, Khanuja SPS. Phylogenetic relationships as in Ocimum revealed by RAPD markers. Euphytica. 2004;136: 11-20.
43. Singh SK, Rai MK, Asthana P, Pandey S, Jaiswal VS, Jaiswal U. Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species. Acta Physiol Plant. 2009;31: 649-53.
44. Singh AK, Sharma M, Varshney R, Agarwal SS, Bansal KC. Plant regeneration from alginate to encapsulated shoot tips of Phyllanthus amarus Schum and Thonn, a medicinally important plant species. In Vitro Cell Dev Biol Plant. 2006;42: 109-13.
45. Smulders MJM. Are there adequate methods for assessing somaclonal variation in tissue culture-propagated plants?. In: Libiakova G, Gajdosova A, editors. COST 843 Final Conference/COST 843 and COST 851 Joint Meeting. Stara Lesna; 2005. pp. 201-203.
46. Sneath PHA, Sokal RR. Numerical Taxonomy: The principles and practices of Numerical classification. San Francisco: W. H. Freeman & Company; 1973.
47. Srivastava V, Khan SA, Banerjee S. An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage. Plant Cell Tissue Organ Cult. 2009;99: 193-8.
48. Sulistiarini DL. Ocimum gratissimum L. In: Oyen APL, Nguyen DX, editors. Plant resources of South-East Asia No. 19: essential oils plants. Bogor: Prosea Foundation; 1999. p. 140-2.
49. Sundararaj SG, Agrawal A, Tyagi RK. Encapsulation for in vitro short-term storage and exchange of ginger (Zingiber officinale Rosc.) germplasm. Sci Hortic. 2010;125: 761-6.
50. Telci I, Bayram E, Yilmaz G, Avci B. Variability in essential oil composition of Turkish basils (Ocimum basilicum L.). Biochem Syst Ecol. 2006;34: 489-97.
51. Vieira RF, Goldsbrough PB, Simon JE. Genetic diversity of basil based on RAPD markers. J Am Soc Hortic Sci. 2003;128: 94-9.
52. Viera RE, Fahoer R, Raina SK. Genetic Diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers. Biochem Syst Ecol. 2001;29: 287-304.
53. Waugh R, Powell W. Using RAPD markers for crop improvement. Trends Biotechnol. 1992;10: 186-91.
54. Wealth of India. A Dictionary of India Raw Materials and Industrial Products Vol. VII (N-Pe). New Delhi: CSRI Publication; 1966.
55. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res. 1990;18: 6531-5.
56. Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 1994;20: 176-83.