Enzymatic and structural characterization of rTSγ provides insights into the function of rTSβ

Biochemistry

Volumn 53, Issue 16, 2014, Pages 2732-2738

  Wichelecki, Daniel J ^a   Froese, D Sean ^b   Kopec, Jolanta ^b   Muniz, Joao R C ^b   Yue, Wyatt W ^b   Gerlt, John A ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CELL CULTURE;

5-FLUOROURACIL; ENZYMATIC ACTIVITIES; HIGH RESOLUTION CRYSTAL STRUCTURE; INFORMED PROPOSALS; OVER-EXPRESSION; RESISTANT CELLS; STRUCTURAL CHARACTERIZATION; THYMIDYLATE SYNTHASE;

GENE ENCODING;

FLUOROURACIL; HYDROLYASE; METHOTREXATE; THYMIDYLATE SYNTHASE;

ARTICLE; CELL LINE; CLONING; CRYSTAL STRUCTURE; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME STRUCTURE; HUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FUNCTION; STEADY STATE;

CRYSTALLOGRAPHY, X-RAY; FLUOROURACIL; HUMANS; HYDRO-LYASES; ISOENZYMES; METHOTREXATE; MODELS, MOLECULAR; PROTEIN CONFORMATION;

EID: 84899635544     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi500349e     Document Type: Article

References (35)

1. Dolnick, B. J. (1993) Cloning and Characterization of a Naturally Occurring Antisense RNA to Human Thymidylate Synthase mRNA Nucleic Acids Res. 21 (8) 1747-1752
2. Dolnick, B. J. and Black, A. R. (1996) Alternate Splicing of the rTS Gene Product and Its Overexpression in a 5-Fluorouracil-resistant Cell Lin Cancer Res. 56, 3207-3210
3. Dolnick, B. J., Lu, K., Yin, M., and Rustum, Y. M. (1997) Recent Advances in the Study of rTS Proteins. rTS Expression During Growth and in Response to Thymidylate Synthase Inhibitors in Human Tumor Cells Advan. Enzyme Regul. 37, 95-109
4. Dolnick, B. J., Black, A. R., Winkler, P. M., Schindler, K., and Hsueh, C. (1996) rTS Gene Expression is Associated with Altered Cell Sensitivity to Thymidylate Synthase Inhibitors Advan. Enzyme Regul. 36, 165-180
5. Lin, Y. L. and Chow, K. C. (2010) rTS Beta as a Novel 5-Fluorouracil Resistance Marker of Colorectal Caner: A Preliminary Study Annals Academy of Medicine Singapore 39, 107-111
6. Srimatkandada, S., Medina, W. D., Cashmore, A. R., Whyte, W., Engel, D., Moroson, B. A., Franco, C. T., Dube, S. K., and Bertino, J. R. (1983) Amplification and organization of dihydrofolate reductase genes in a human leukemic cell line, K-562, resistant to methotrexate Biochemistry 56, 5774-81
7. Black, A. R. and Dolnick, B. J. (1996) Expression of rTS correlates with altered growth regulation of thymidylate synthase Cancer Res. 56, 700-5
8. Kuo, S. J., Wang, H. C., Chow, K. C., Chiou, S. H., Chiang, S. F., Lin, T. Y., Chiang, I. P., and Chen, D. R. (2008) Expression of rTSβ as a 5-Fluorouracil Resistance marker in patients with Primary Breast Cancer Oncol. Rep. 19, 881-888
9. Liang, P., Nair, J. R., Song, L., McGuire, J. J., and Dolnick, B. J. (2005) Comparative Genomic Analysis Reveals a Novel Mitochondrial Isoform of Human rTS protein and Unusual Phylogenetic Distribution of the rTS Gene BMC Genomics 6 (125 10.1186/1471-2164-6-125
10. Gerlt, J. A., Babbitt, P. C., and Rayment, I. (2005) Divergent Evolution in the Enolase Superfamily: The Interplay of Mechanism and Specificity Arch. Biochem. Biophys. 433, 59-70
11. Babbitt, P. C., Mrachko, G. T., Hasson, M. S., Huisman, G. W., Kolter, R., Ringe, D., Petsko, G. A., Kenyon, G. L., and Gerlt, J. A. (1995) A Functionally Diverse Enzyme Superfamily that Abstracts the Alpha Protons of Carboxylic Acids Science 267, 1159-1161
12. Babbitt, P. C., Hasson, M. S., Wedekind, J. E., Palmer, D. R. J., Barret, W. C., Reed, G. H., Rayment, I., Ringe, D., Kenyon, G. L., and Gerlt, J. A. (1996) The Enolase Superfamily: A General Strategy for Enzyme-Catalyzed Abstraction of the alpha-Protons of Carboxylic Acids Biochemistry 35, 16489-16501
13. Glasner, M. E., Gerlt, J. A., and Babbitt, P. C. (2006) Evolution of Enzyme Superfamilies Curr. Opin. Chem. Biol. 10, 492-497
14. Kabsch, W. (2010) Integration, scaling, space-group assignment and post-refinement Acta Crystallogr. D66, 133-144
15. Collaborative Computational Project, Number 4 (1994) The CCP4 suite: programs for protein crystallography Acta Crystallogr. D50, 760-763
16. Sheldrick, G. M. (2008) A short history of SHELX Acta Crystallogr. A64, 112-122
17. Vonrhein, C., Blanc, E., Roversi, P., and Bricogne, G. (2007) Automated structure solution with autoSHARP Methods Mol. Biol. 364, 215-230
18. Abrahams, J. P. and Leslie, A. G. W. (1996) Methods used in the structure determination of bovine mitochondrial F1 ATPase Acta Crystallogr. D52, 30-42
19. Cowtan, K. (2006) The Buccaneer software for automated model building. 1. Tracing protein chains Acta Crystallogr. D62, 1002-1011
20. Emsley, P. and Cowtan, K. D. (2004) Coot: model-building tools for molecular graphics Acta Crystallogr. D60, 2126-2132
21. Gulick, A. M., Hubbard, B. K., Gerlt, J. A., and Rayment, I. (2001) Evolution of Enzymatic Activities in the Enolase Superfamily: Identification of the General Acid Catalyst in the Active Site of D-Glucarate Dehydratase from Escherichia coli Biochemistry 40, 10054-10062
22. Olson, J. A. (1959) Spectrophotometric measurement of alpha-keto acid semicarbazones Arch. Biochem. Biophys. 85, 225-233
23. Yew, W. S., Fedorov, A. A., Fedorov, E. V., Rakus, J. F., Pierce, R. W., Almo, S. C., and Gerlt, J. A. (2006) Evolution of Enzymatic Activities in the Enolase Superfamily: l -fuconate Dehydratase from Xanthomonas campestris Biochemistry 45, 14582-14597
24. Niesen, F. H., Berglund, H., and Vedadi, M. (2007) The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability Nat Protoc. 2, 2212-21
25. Wichelecki, D. J., Balthazor, B. M., Chau, A. A., Vetting, M. W., Fedorov, A. A., Fedorov, E. V., Lukk, T., Patskovsky, Y. V., Stead, M. B., Hillerich, B. S., Seidel, R. D., Almo, S. C., and Gerlt, J. A. (2014) Discovery of function in the enolase superfamily: D-mannonate and D-gluconate dehydratases in the D-mannonate dehydratase subgroup Biochemistry 10.1021/bi500264p
26. Yuen, R. and Schachter, H. (1972) L-Fucose metabolism in mammals. I. Port liver l -fuconate hydro-lyase Can. J. Biochem. 50, 798-806
27. Becker, D. J. and Lowe, J. B. (2003) Fucose: biosynthesis and biological function in mammals Glycobiology 13, 41R-53R
28. Neidhard, D. J., Howell, P. L., Petsko, G. A., Powers, V. M., Li, R. S., Kenyon, G. L., and Gerlt, J. A. (1991) Mechanism of the Reaction Catalyzed by Mandelate Racemase. 2. Crystal Structure of Mandelate Racemase at 2.5-Å resolution: Identification of the Active Site and Possible Catalytic Residues Biochemistry 30, 9264-9273
29. Gerlt, J. A. and Raushel, F. M. (2003) Evolution of function in (beta/alpha)(8)-barrel enzymes Curr. Opin. Chem. Biol. 7, 252-264
30. Yew, W. S., Fedorov, A. A., Fedorov, E. V., Wood, B. M., Almo, S. C., and Gerlt, J. A. (2006) Evolution of Enzymatic Activities in the Enolase Superfamily: D-Tartrate Dehydratase from Bradyrhizobium japonicum Biochemistry 45, 14598-145608
31. Yew, W. S., Fedorov, A. A., Fedorov, E. V., Almo, S. C., and Gerlt, J. A. (2007) Evolution of Enzymatic Activities in the Enolase Superfamily: L-Talarate/Galactarate Dehydratase from Salmonella typhimurium LT2 Biochemistry 46, 9564-9577
32. Rakus, J. F., Fedorov, A. A., Fedorov, E. V., Glasner, M. E., Hubbard, B. K., Delli, J. D., Babbitt, P. C., Almo, S. C., and Gerlt, J. A. (2008) Evolution of enzymatic activities in the enolase superfamily: L-rhamnonate dehydratase Biochemistry 47, 9944-9954
33. Dolnick, B. J., Angelino, N. J., Dolnick, R., and Sufrin, J. R. (2003) A Novel Function for the rTS Gene Cancer Biology & Therapy 2 (4) 364-369
34. Dolnick, R., Wu, Q., Angelino, N. J., Stephanie, L. V., Chow, K., Sufrin, J. R., and Dolnick, B. J. (2005) Enhancement of 5-Fluorouracil Sensitivity by an rTS Signaling Mimic in H630 Colon Cancer Cells Cancer Res. 65 (13) 5917-5924
35. Dolnick, B. J. (1996) Proteins encoded by the antisense strand gene (rTS) of thymidylate synthase interact with thymidylate synthase and dihydrofolate reductase Proc. Am. Assoc. Cancer Res. 37, 652-653