Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions

PLoS ONE

Volumn 9, Issue 4, 2014, Pages

  Durruthy Durruthy, Jens ^a   Briggs, Sharon F ^a   Awe, Jason ^b,c   Ramathal, Cyril Y ^a   Karumbayaram, Saravanan ^b,d   Lee, Patrick C ^b,c   Heidmann, Julia D ^e   Clark, Amander ^b,d   Karakikes, Ioannis ^a,e   Loh, Kyle M ^e   Wu, Joseph C ^a,e   Hoffman, Andrew R ^e   Byrne, James ^b,c   Reijo Pera, Renee A ^a   Sebastiano, Vittorio ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA FETOPROTEIN; CELL SURFACE MARKER; DESMIN; MESSENGER RNA; OCTAMER TRANSCRIPTION FACTOR 4; ORGANIC CATION TRANSPORTER 3; STAGE SPECIFIC EMBRYO ANTIGEN 3; STAGE SPECIFIC EMBRYO ANTIGEN 4; TRA 1 60 SURFACE MARKER; TRA 1 81 SURFACE MARKER; TRANSCRIPTION FACTOR NANOG; UNCLASSIFIED DRUG; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; KRUPPEL LIKE FACTOR; KRUPPEL LIKE FACTOR 4; LIN28B PROTEIN, HUMAN; MYC PROTEIN; MYC PROTEIN, HUMAN; POU5F1 PROTEIN, HUMAN; RNA BINDING PROTEIN; SOX2 PROTEIN, HUMAN; TRANSCRIPTION FACTOR SOX;

ADULT; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL CLONE; CELL DENSITY; CELL DIFFERENTIATION; CELL STRUCTURE; CONTROLLED STUDY; CULTURE TECHNIQUE; FEMALE; FETUS; FIBROBLAST CULTURE; GOOD MANUFACTURING PRACTICE; HUMAN; HUMAN CELL; MALE; MIDDLE AGED; MOUSE; NEWBORN; NONHUMAN; NUCLEAR REPROGRAMMING; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; STEM CELL CULTURE; ANIMAL; CELL LINE; CYTOLOGY; DRUG EFFECTS; EMBRYOID BODY; FIBROBLAST; GENE EXPRESSION PROFILING; GENETIC TRANSFECTION; GENETICS; GERM LAYER; ISOLATION AND PURIFICATION; PATHOLOGY; PRIMARY CELL CULTURE; PROCEDURES; SCID MOUSE; SKIN; STANDARDS; SYNTHESIS; TERATOMA; TRANSPLANTATION;

ADULT; ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CELLULAR REPROGRAMMING; EMBRYOID BODIES; FEMALE; FIBROBLASTS; GENE EXPRESSION PROFILING; GERM LAYERS; GREEN FLUORESCENT PROTEINS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; INFANT, NEWBORN; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MALE; MICE; MICE, SCID; MIDDLE AGED; OCTAMER TRANSCRIPTION FACTOR-3; PRIMARY CELL CULTURE; PROTO-ONCOGENE PROTEINS C-MYC; RNA, MESSENGER; RNA-BINDING PROTEINS; SKIN; SOXB1 TRANSCRIPTION FACTORS; TERATOMA; TRANSFECTION;

EID: 84899550919     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0094231     Document Type: Article

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