Modified CLSI extended-spectrum β-lactamase (ESBL) confirmatory test for phenotypic detection of ESBLs among Enterobacteriaceae producing various β-lactamases

Journal of Clinical Microbiology

Volumn 52, Issue 5, 2014, Pages 1483-1489

  Poulou, Aggeliki ^a,b   Grivakou, Evgenia ^a   Vrioni, Georgia ^a   Koumaki, Vassiliki ^a   Pittaras, Theodoros ^a   Pournaras, Spyros ^a   Tsakris, Athanassios ^a  

^a UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

^b General District Hospital of Serres   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIOPHAGE; BACTERIOPHAGE T13; BACTERIOPHAGE T13A; BACTERIOPHAGE T8; BACTERIOPHAGE TYPING; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; DISEASE ASSOCIATION; FOOD POISONING; GENE IDENTIFICATION; GENE LOCUS; GENETIC VARIABILITY; HUMAN; NONHUMAN; NORTH AMERICA; NUCLEOTIDE SEQUENCE; PHYLOGENY; PLASMID; PRIORITY JOURNAL; SALMONELLA ENTERITIDIS; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; SYNAPOMORPHY; UNINDEXED SEQUENCE; ANTIBIOTIC RESISTANCE; CHEMISTRY; COMPARATIVE STUDY; DRUG EFFECTS; ENTEROBACTERIACEAE; ENTEROBACTERIACEAE INFECTIONS; ENZYMOLOGY; GENETICS; MICROBIAL SENSITIVITY TEST; PROCEDURES;

AMPC BETA-LACTAMASES; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; BETA LACTAMASE; CEFOTAXIME; CEFTAZIDIME;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BETA-LACTAM RESISTANCE; BETA-LACTAMASES; CEFOTAXIME; CEFTAZIDIME; ENTEROBACTERIACEAE; ENTEROBACTERIACEAE INFECTIONS; HUMANS; MICROBIAL SENSITIVITY TESTS;

EID: 84899509470     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03361-13     Document Type: Article

References (38)

1. Bush K, Fisher JF. 2011. Epidemiological expansion, structural studies, and clinical challenges of new β-lactamases from Gram-negative bacteria. Annu. Rev. Microbiol. 65:455-478. http://dx.doi.org/10.1146/annurev-micro- 090110-102911.
2. Pitout JD, Laupland KB. 2008. Extended-spectrum β-lactamase- producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect. Dis. 8:159-166. http://dx.doi.org/10.1016/S1473-3099 (08) 70041-0.
3. Zahar JR, Lortholary O, Martin C, Potel G, Plesiat P, Nordmann P. 2009. Addressing the challenge of extended-spectrum β-lactamases. Curr. Opin. Investig. Drugs 10:172-180.
4. Carattoli A. 2009. Resistance plasmid families in Enterobacteriaceae. Antimicrob. Agents Chemother. 53:2227-2238. http://dx.doi.org/10.1128/AAC.01707- 08.
5. Clinical and Laboratory Standards Institute. 2012. Performance standards for antimicrobial susceptibility testing. Twenty second informational supplement update. CLSI document M100-S22 U. Clinical and Laboratory Standards Institute, Wayne, PA.
6. Drieux L, Brossier F, Sougakoff W, Jarlier V. 2008. Phenotypic detection of extended spectrum β-lactamase production in Enterobacteriaceae: review and bench guide. Clin. Microbiol. Infect. 14(Suppl 1):90-103. http://dx.doi.org/10.1111/j.1469-0691.2007.01846.x.
7. Tzelepi E, Giakkoupi P, Sofianou D, Loukova V, Kemeroglou A, Tsakris A. 2000. Detection of extended-spectrum β-lactamases in clinical isolates of Enterobacter cloacae and Enterobacter aerogenes. J. Clin. Microbiol. 38:542-546.
8. Garrec H, Drieux-Rouzet L, Golmard JL, Jarlier V, Robert J. 2011. Comparison of nine phenotypic methods for detection of extendedspectrum β-lactamase production by Enterobacteriaceae. J. Clin. Microbiol. 49:1048-1057. http://dx.doi.org/10.1128/JCM.02130-10.
9. Stuart JC, Diederen B, Al Naiemi N, Fluit A, Arents N, Thijsen S, Vlaminckx B, Mouton JW, Leverstein-van Hall M. 2011. Method for phenotypic detection of extended-spectrum β-lactamases in enterobacter species in the routine clinical setting. J. Clin. Microbiol. 49:2711-2713. http://dx.doi.org/ 10.1128/JCM.00864-11.
10. Nordmann P, Dortet L, Poirel L. 2012. Rapid detection of extendedspectrum-β-lactamase-producing Enterobacteriaceae. J. Clin. Microbiol. 50:3016-3022. http://dx.doi.org/10.1128/JCM.00859-12.
11. Coudron PE. 2005. Inhibitor-based methods for detection of plasmidmediated AmpC β-lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis. J. Clin. Microbiol. 43:4163-4167. http://dx.doi.org/10.1128/ JCM.43.8.4163-4167.2005.
12. Doi Y, Potoski BA, Adams-Haduch JM, Sidjabat HE, Pasculle AW, Paterson DL. 2008. Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type β-lactamase by use of a boronic acid compound. J. Clin. Microbiol. 46:4083-4086. http://dx.doi.org/10.1128/JCM.01408-08.
13. Pitout JD, Le PG, Moore KL, Church DL, Gregson DB. 2010. Detection of AmpC β-lactamases in Escherichia coli, Klebsiella spp., Salmonella spp and Proteus mirabilis in a regional clinical microbiology laboratory. Clin. Microbiol. Infect. 16:165-170. http://dx.doi.org/10.1111/j.1469-0691.2009.02756. x.
14. Tsakris A, Kristo I, Poulou A, Markou F, Ikonomidis A, Pournaras S. 2008. First occurrence of KPC-2-possessing Klebsiella pneumoniae in a Greek hospital and recommendation for detection with boronic acid disc tests. J. Antimicrob. Chemother. 62:1257-1260. http://dx.doi.org/10.1093/jac/dkn364.
15. Giske CG, Gezelius L, Samuelsen Ø Warner M, Sundsfjord A, Woodford N. 2011. A sensitive and specific phenotypic assay for detection of metallo-β-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. Clin. Microbiol. Infect. 17:552-556. http://dx.doi.org/10.1111/j. 1469-0691.2010.03294.x.
16. Jeong SH, Song W, Park MJ, Kim JS, Kim HS, Bae IK, Lee KM. 2008. Boronic acid disk tests for identification of extended-spectrum β-lactamase production in clinical isolates of Enterobacteriaceae producing chromosomal AmpC β-lactamases. Int. J. Antimicrob. Agents 31:467-471. http://dx.doi.org/10. 1016/j.ijantimicag.2007.12.014.
17. Song W, Bae IK, Lee YN, Lee CH, Lee SH, Jeong SH. 2007. Detection of extended-spectrum β-lactamases by using boronic acid as an AmpC β-lactamase inhibitor in clinical isolates of Klebsiella spp and Escherichia coli. J. Clin. Microbiol. 45:1180-1184. http://dx.doi.org/10.1128/ JCM.02322-06.
18. Tsakris A, Poulou A, Themeli-Digalaki K, Voulgari E, Pittaras T, Sofianou D, Pournaras S, Petropoulou D. 2009. Use of boronic acid disk tests to detect extended-spectrum β-lactamases in clinical isolates of KPC carbapenemase-possessing Enterobacteriaceae. J. Clin. Microbiol. 47:3420-3426. http://dx.doi.org/10.1128/JCM.01314-09.
19. Tsakris A, Poulou A, Pournaras S, Voulgari E, Vrioni G, Themeli-Digalaki K, Petropoulou D, Sofianou D. 2010. A simple phenotypic method for the differentiation of metallo-β-lactamases and class A KPC carbapenemases in Enterobacteriaceae clinical isolates. J. Antimicrob. Chemother. 65:1664-1671. http://dx.doi.org/10.1093/jac/dkq210.
20. Nordmann P, Gniadkowski M, Giske CG, Poirel L, Woodford N, Miriagou V; European Network on Carbapenemases. 2012. Identification and screening of carbapenemase-producing Enterobacteriaceae. Clin. Microbiol. Infect. 18:432-438. http://dx.doi.org/10.1111/j.1469-0691.2012.03815.x.
21. Franklin C, Liolios L, Peleg AY. 2006. Phenotypic detection of carbapenemsusceptible metallo-β-lactamase-producing gram-negative bacilli in the clinical laboratory. J. Clin. Microbiol. 44:3139-3144. http://dx.doi.org/10.1128/JCM.00879-06.
22. Pournaras S, Poulou A, Tsakris A. 2010. Inhibitor-based methods for the detection of KPC carbapenemase-producing Enterobacteriaceae in clinical practice by using boronic acid compounds. J. Antimicrob. Chemother. 65:1319-1321. http://dx.doi.org/10.1093/jac/dkq124.
23. Tzelepi E, Magana C, Platsouka E, Sofianou D, Paniara O, Legakis NJ, Vatopoulos AC, Tzouvelekis LS. 2003. Extended-spectrum β-lactamase types in Klebsiella pneumoniae and Escherichia coli in two Greek hospitals. Intern. J. Antimicrob. Agents 21:285-288. http://dx.doi.org/10.1016/S0924-8579 (02) 00361-8.
24. Smith Moland E, Hanson ND, Herrera VL, Black JA, Lockhart TJ, Hossain A, Johnson JA, Goering RV, Thomson KS. 2003. Plasmidmediated, carbapenem- hydrolysing β-lactamase, KPC-2, in Klebsiella pneumoniae isolates. J. Antimicrob. Chemother. 51:711-714. http://dx.doi.org/10.1093/jac/dkg124.
25. Poirel L, Héritier C, Tolün V, Nordmann P. 2004. Emergence of oxacillinasemediated resistance to imipenem in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 48:15-22. http://dx.doi.org/10.1128/AAC.48.1.15- 22.2004.
26. Agodi A, Voulgari E, Barchitta M, Politi L, Koumaki V, Spanakis N, Giaquinta L, Valenti G, Romeo MA, Tsakris A. 2011. Containment of an outbreak of KPC-3-producing Klebsiella pneumoniae in Italy. J. Clin. Microbiol. 49:3986-3989. http://dx.doi.org/10.1128/JCM.01242-11.
27. Pérez-Pérez FJ, Hanson ND. 2002. Detection of plasmid-mediatedAmpCβ-lactamase genes in clinical isolates by using multiplex PCR. J. Clin. Microbiol. 40:2153-2162. http://dx.doi.org/10.1128/JCM. 40.6.2153-2162.2002.
28. Preston KE, Radomski CC, Venezia RA. 2000. Nucleotide sequence of the chromosomal ampC gene of Enterobacter aerogenes. Antimicrob. Agents Chemother. 44:3158-3162. http://dx.doi.org/10.1128/AAC.44.11.3158-3162.2000.
29. Barnaud G, Labia R, Raskine L, Sanson-Le Pors MJ, Philippon A, Arlet G. 2001. Extension of resistance to cefepime and cefpirome associated to a six amino acid deletion in the H-10 helix of the cephalosporinase of an Enterobacter cloacae clinical isolate. FEMS Microbiol. Lett. 195:185-190. http://dx.doi.org/10.1111/j.1574-6968.2001.tb10519.x.
30. Tsakris A, Kristo I, Poulou A, Themeli-Digalaki K, Ikonomidis A, Petropoulou D, Pournaras S, Sofianou D. 2009. Evaluation of boronic acid disk tests for differentiating KPC-possessing Klebsiella pneumoniae isolates in the clinical laboratory. J. Clin. Microbiol. 47:362-367. http://dx.doi.org/10.1128/ JCM.01922-08.
31. Poulou A, Voulgari E, Vrioni G, Koumaki V, Xidopoulos G, Chatzipantazi V, Markou F, Tsakris A. 2013. Outbreak caused by an ertapenemresistant, CTX-M-15-producing Klebsiella pneumoniae sequence type 101 clone carrying an OmpK36 porin variant. J. Clin. Microbiol. 51:3176-3182. http://dx.doi.org/10. 1128/JCM.01244-13.
32. Rossolini GM, Mantengoli E, Docquier JD, Musmanno RA, Coratza G. 2007. Epidemiology of infections caused by multiresistant gramnegatives: ESBLs, MBLs, panresistant strains. New Microbiol. 30:332-339.
33. Livermore DM. 2009. Has the era of untreatable infections arrived? J. Antimicrob. Chemother. 64:29-36. http://dx.doi.org/10.1093/jac/dkp255.
34. KPC-containing Klebsiella pneumoniae isolates detected in different institutions in the Eastern USA. J. Antimicrob. Chemother. 63:427-437. http://dx.doi.org/10.1093/jac/ dkn547.
35. Pournaras S, Poulou A, Voulgari E, Vrioni G, Kristo I, Tsakris A. 2010. Detection of the new metallo-β-lactamase VIM-19 along with KPC-2, CMY-2 and CTX-M-15 in Klebsiella pneumoniae. J. Antimicrob. Chemother. 65:1604-1607. http://dx.doi.org/10.1093/jac/dkq190.
36. Livermore DM, Andrews JM, Hawkey PM, Ho PL, Keness Y, Doi Y, Paterson D, Woodford N. 2012. Are susceptibility tests enough, or should laboratories still seek ESBLs and carbapenemases directly? J. Antimicrob. Chemother. 67:1569-1577. http://dx.doi.org/10.1093/jac/dks088.
37. Poirel L, Potron A, Nordmann P. 2012. OXA-48-like carbapenemases: the phantom menace. J. Antimicrob. Chemother. 67:1597-1606. http://dx.doi.org/10. 1093/jac/dks121.
38. Robberts FJ, Kohner PC, Patel R. 2009. Unreliable extended-spectrum β-lactamase detection in the presence of plasmid-mediated AmpC in Escherichia coli clinical isolates. J. Clin. Microbiol. 47:358-361. http://dx.doi.org/10.1128/JCM.01687-08.