NeuroArray: A universal interface for patterning and interrogating neural circuitry with single cell resolution

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Li, Wei ^a   Xu, Zhen ^a   Huang, Junzhe ^a   Lin, Xudong ^a   Luo, Rongcong ^b   Chen, Chia Hung ^b   Shi, Peng ^a  

^a CITY UNIVERSITY OF HONG KONG   (Hong Kong)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; ARTICLE; CULTURE TECHNIQUE; EQUIPMENT; METHODOLOGY; MICROARRAY ANALYSIS; NERVE CELL; NERVE CELL NETWORK; PHYSIOLOGY; PYRAMIDAL NERVE CELL; RAT; SINGLE CELL ANALYSIS;

ANIMALS; CELL CULTURE TECHNIQUES; MICROARRAY ANALYSIS; NERVE NET; NEURONS; PYRAMIDAL CELLS; RATS; SINGLE-CELL ANALYSIS;

EID: 84899472319     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep04784     Document Type: Article

References (29)

1. Hua, J. Y. Y. & Smith, S. J. Neural activity and the dynamics of central nervous system development. Nature Neuroscience 7, 327-332 (2004
2. Palop, J. J., Chin, J. & Mucke, L. A network dysfunction perspective on neurodegenerative diseases. Nature 443, 768-773 (2006
3. Shahaf, G. & Marom, S. Learning in networks of cortical neurons. J Neurosci 21, 8782-8788 (2001
4. Dertinger, S. K., Jiang, X., Li, Z., Murthy, V. N. & Whitesides, G. M. Gradients of substrate-bound laminin orient axonal specification of neurons. Proc Natl Acad Sci U.S.A 99, 12542-12547 (2002
5. Martin, K. C. et al. Synapse-specific, long-term facilitation of aplysia sensory to motor synapses: A function for local protein synthesis in memory storage. Cell 91, 927-938 (1997
6. Shi, P. et al. Synapse microarray identification of small molecules that enhance synaptogenesis. Nat Commun 2, 510 (2011
7. Jain, S. &Heutink, P. From single genes to gene networks: High-throughput-highcontent screening for neurological disease. Neuron 68, 207-217 (2010
8. Feinerman, O., Rotem, A. & Moses, E. Reliable neuronal logic devices from patterned hippocampal cultures. Nature Physics 4, 967-973 (2008
9. Kleinfeld, D., Kahler, K. H. & Hockberger, P. E. Controlled outgrowth of dissociated neurons on patterned substrates. J Neurosci 8, 4098-4120 (1988
10. Branch, D. W., Wheeler, B. C., Brewer, G. J. & Leckband, D. E. Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine. IEEE Trans Biomed Eng 47, 290-300 (2000
11. Kam, L., Shain, W., Turner, J. N. & Bizios, R. Axonal outgrowth of hippocampal neurons on micro-scale networks of polylysine-conjugated laminin. Biomaterials 22, 1049-1054 (2001
12. Shi, P., Shen, K. & Kam, L. C. Local presentation of L1 and N-cadherin in multicomponent, microscale patterns differentially direct neuron function in vitro. Dev Neurobiol 67, 1765-1776 (2007
13. Shi, P., Nedelec, S., Wichterle, H. & Kam, L. C. Combined microfluidics/protein patterning platform for pharmacological interrogation of axon pathfinding. Lab Chip 10, 1005-1010 (2010
14. Taylor, A. M. et al. A microfluidic culture platform for CNS axonal injury, regeneration and transport. Nat Methods 2, 599-605 (2005
15. Dworak, B. J. & Wheeler, B. C. Novel MEA platform with PDMS microtunnels enables the detection of action potential propagation from isolated axons in culture. Lab Chip 9, 404-410 (2009
16. Wheeler, B. C. & Brewer, G. J. Designing Neural Networks in Culture: Experiments are described for controlled growth, of nerve cells taken from rats, in predesigned geometrical patterns on laboratory culture dishes. Proc IEEE Inst Electr Electron Eng 98, 398-406 (2010
17. Qin, D., Xia, Y. & Whitesides, G. M. Soft lithography for micro-and nanoscale patterning. Nat Protoc 5, 491-502 (2010
18. Whitesides, G. M., Ostuni, E., Takayama, S., Jiang, X. & Ingber, D. E. Soft lithography in biology and biochemistry. Annu Rev Biomed Eng 3, 335-373 (2001
19. Heo, Y. S. et al. Characterization and resolution of evaporation-mediated osmolality shifts that constrain microfluidic cell culture in poly(dimethylsiloxane) devices. Anal Chem 79, 1126-1134 (2007
20. Folch, A., Jo, B. H., Hurtado, O., Beebe, D. J. & Toner, M. Microfabricated elastomeric stencils for micropatterning cell cultures. J Biomed Mater Res 52, 346-353 (2000
21. Choi, J. H., Lee, H., Jin, H. K., Bae, J. S. & Kim, G. M. Micropatterning of neural stem cells and Purkinje neurons using a polydimethylsiloxane (PDMS) stencil. Lab Chip 12, 5045-5050 (2012
22. Chen, W., Lam, R. H. & Fu, J. Photolithographic surface micromachining of polydimethylsiloxane (PDMS). Lab Chip 12, 391-395 (2012
23. Buzsaki, G., Logothetis, N. & Singer, W. Scaling brain size, keeping timing: Evolutionary preservation of brain rhythms. Neuron 80, 751-764 (2013
24. Vogelstein, J. T. et al. Fast nonnegative deconvolution for spike train inference from population calcium imaging. J Neurophysiol 104, 3691-3704 (2010
25. Ohki, K., Chung, S., Ch?ng, Y. H., Kara, P. & Reid, R. C. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature 433, 597-603 (2005
26. Eytan, D. & Marom, S. Dynamics and effective topology underlying synchronization in networks of cortical neurons. JNeurosci 26, 8465-8476 (2006
27. Kang,G., Lee, J. H., Lee, C. S.&Nam, Y. Agarose microwell based neuronal microcircuit arrays on microelectrode arrays for high throughput drug testing. Lab Chip 9, 3236-3242 (2009
28. Luo, R. C., Ranjan, S., Zhang, Y. & Chen, C. H. Near-infrared photothermal activation of microgels incorporating polypyrrole nanotransducers through droplet microfluidics. Chem Commun 49, 7887-7889 (2013
29. Kaech, S. & Banker, G. Culturing hippocampal neurons. Nat Protoc 1, 2406-2415 (2006).