Quantitative X-ray elemental imaging in plant materials at the subcellular level with a transmission electron microscope: Applications and limitations

Materials

Volumn 7, Issue 4, 2014, Pages 3160-3175

  Chen, Shaoliang ^a   Diekmann, Heike ^b   Janz, Dennis ^b   Polle, Andrea ^b  

^a BEIJING FORESTRY UNIVERSITY   (China)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Agar standard; Cell wall; Cortex; EDX; Populus euphratica; Root; Salt tolerance; TEM; Vacuole; Xylem vessel

Indexed keywords

ENERGY DISPERSIVE SPECTROSCOPY; HISTOLOGY; POLYSACCHARIDES; TISSUE; TRANSMISSION ELECTRON MICROSCOPY;

CELL WALLS; CORTEX; POPULUS EUPHRATICA; ROOT; SALT TOLERANCE; VACUOLE; XYLEM VESSELS;

ALGAE;

EID: 84899134115     PISSN: None     EISSN: 19961944     Source Type: Journal    
DOI: 10.3390/ma7043160     Document Type: Article

References (32)

1. Ryan, M.H.; McCully, M.E.; Huang, C.X. Relative amounts of soluble and insoluble forms of phosphorus and other elements in intraradical hyphae and arbuscules of arbuscular mycorrhizas. Funct. Plant Biol. 2007, 34, 457-464.
2. McCully, M.E.; Canny, M.J.; Huang, C.X.; Miller, C.; Brink, F. Cryo-scanning electron microscopy (CSEM) in the advancement of functional plant biology: energy dispersive X-ray microanalysis (CEDX) applications. Funct. Plant Biol. 2010, 37, 1011-1040.
3. Sauberman, A.J.; Heyman, R.V. Quantitative digital X-ray imaging using frozen hydrated and frozen dried tissue sections. J. Microsc. 1987, 146, 169-182.
4. LeFurgey, A.; Davilla, S.D.; Kopf, D.A.; Sommer, J.R.; Ingram, P. Real-time quantitative elemental analysis and mapping: Microchemical imaging in cell physiology. J. Microsc. 1992, 165, 191-223.
5. Bidwell, S.D.; Crawford, S.A.; Woodrow, I.E.; Sommer-Knudsen, J.; Marshall, A.T. Sub-cellular localization of Ni in the hyperaccumulator, Hybanthus floribundus (Lindley) F. Muell. Plant Cell Environ. 2004, 27, 705-716.
6. Fritz, E. X-ray microanalysis of diffusible elements in plant cells after freeze-drying, pressure-infiltration with ether and embedding in plastic. Scanning Microsc. 1989, 3, 517-526.
7. Fritz, E.; Jentschke, G. Agar standards for quantitative X-ray microanalysis of resin-embedded plant tissues. J. Microsc. 1994, 174, 47-50.
8. Fritz, E. Measurement of cation exchange capacity (CEC) of plant cell walls by X-ray microanalysis (EDX) in the transmission electron microscope. Microsc. Microanal. 2007, 13, 233-244.
9. Marshall, A.T.; Xu, W. Quantitative elemental X-ray imaging of frozen-hydrated biological samples. J. Microsc. 1998, 190, 305-316.
10. Marshall, A.T.; Goodyear, M.J.; Crewther, S.G. Sequential quantitative X-ray elemental imaging of frozen-hydrated and freeze-dried biological bulk samples in the SEM. J. Microsc. 2012, 245, 17-25.
11. Chen, S.; Li, J.; Wang, S.; Hüttermann, A.; Altman, A. Salt, nutrient uptake and transport, and ABA of Populus euphratica: A hybrid in response to increasing soil NaCl. Trees Struct. Funct. 2001, 15, 186-194.
12. Chen, S.; Li, J.; Wang, T.; Wang, S.; Polle, A.; Hüttermann, A. Osmotic stress and ion-specific effects on xylem abscisic acid and the relevance to salinity tolerance in poplar. J. Plant Growth Regul. 2002, 21, 224-233.
13. Gu, R.; Fonseca, S.; Puskás, L.G.; Hackler, L.J.; Zvara, A.; Dudits, D.; Pais, M.S. Transcript identification and profiling during salt stress and recovery of Populus euphratica. Tree Physiol. 2004, 24, 265-276.
14. Ottow, E.A.; Brinker, M.; Teichmann, T.; Fritz, E.; Kaiser, W.; Brosché, M.; Kangasjärvi, J.; Jiang, X.; Polle, A. Populus euphratica displays apoplastic sodium accumulation, osmotic adjustment by decreases in calcium and soluble carbohydrates, and develops leaf succulence under salt stress. Plant Physiol. 2005, 139, 1762-1772.
15. Wang, R.; Chen, S.; Deng, L.; Fritz, E.; Hüttermann, A.; Polle, A. Leaf photosynthesis, fluorescence response to salinity and the relevance to chloroplast salt compartmentation and anti-oxidative stress in two poplars. Trees Struct. Funct. 2007, 21, 581-591.
16. Wang, R.; Chen, S.; Zhou, X.; Shen, X.; Deng, L.; Zhu, H.; Shao, J.; Shi, Y.; Dai, S.; Fritz, E.; et al. Ionic homeostasis and reactive oxygen species control in leaves and xylem sap of two poplars subjected to NaCl stress. Tree Physiol. 2008, 28, 947-957.
17. 2 and cytosolic Ca2+ in the salt response of Populus euphratica cells. PLoS ONE 2012, 7, e53136.
18. Ding, M.; Hou, P.; Shen, X.; Wang, M.; Deng, S.; Sun, J.; Xiao, F.; Wang, R.; Zhou, X.; Lu, C.; et al. Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species. Plant Mol. Biol. 2010, 73, 251-269.
19. Janz, D.; Behnke, K.; Schnitzler, J.P.; Kanawati, B.; Schmitt-Kopplin, P.; Polle, A. Pathway analysis of the transcriptome and metabolome of salt sensitive and tolerant poplar species reveals evolutionary adaption of stress tolerance mechanisms. BMC Plant Biol. 2010, 10, doi:10.1186/1471-2229-10-150.
20. Chen, S.; Polle, A. Salinity tolerance of Populus. Plant Biol. 2010, 12, 317-333.
21. Chen, S.; Li, J.; Fritz, E.; Wang, S.; Hüttermann, A. Sodium and chloride distribution in roots and transport in three poplar genotypes under increasing NaCl stress. Forest Ecol. Manage. 2002, 168, 217-230.
22. Chen, S.; Li, J.; Wang, S.; Fritz, E.; Hüttermann, A.; Altman, A. Effects of NaCl on shoot growth, transpiration, ion compartmentation and transport in regenerated plants of Populus euphratica and Populus tomentosa. Can. J. Forest Res. 2003, 33, 967-975.
23. Chen, S.; Zommorodi, M.; Fritz, E.; Wang, S.; Hüttermann, A. Hydrogel modified uptake of salt ions and calcium in Populus euphratica under saline conditions. Trees Struct. Funct. 2004, 18, 175-183.
24. +-ATPase and the relevance to salt tolerance in two contrasting poplar species. Trees Struct. Funct. 2010, 24, 597-607.
25. Chen, S.; Olbrich, A.; Langenfeld-Heyser, R.; Fritz, E.; Polle, A. Quantitative X-ray microanalysis of hydrogen peroxide within plant cells. Microsc. Res. Tech. 2009, 72, 49-60.
26. Sun, J.; Chen, S.; Dai, S.; Wang, R.; Li, N.; Shen, X.; Zhou, X.; Lu, C.; Zheng, X.; Hu, Z.; et al. NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar. Plant Physiol. 2009, 149, 1141-1153.
27. + homeostasis in poplar species differing in salt tolerance. Tree Physiol. 2009, 29, 1175-1186.
28. + homeostasis and antioxidant defense in NaCl-stressed callus cells of two contrasting poplars. Plant Cell Tissue Organ Cult. 2010, 103, 205-215.
29. + homeostasis in NaCl-stressed Populus euphratica cells. Plant Cell Environ. 2010, 33, 943-958.
30. Shabala, S.; Cuin, T.A. Potassium transport and plant salt tolerance. Physiol. Plant 2008, 133, 651-669.
31. Leplé, J.C.; Brasiliero, A.; Michel, M.F.; Delmotte, F.; Jouanin, L. Transgenic poplars: Expression of chimeric genes using four different constructs. Plant Cell Rep. 1992, 11, 137-141.
32. Kriz, W.; Schnermann, J.; Höhling, H.J.; Von Rosenstiel, A.P.; Hall, T.A. Electron probe microanalysis of electrolytes in kidney cells. In Recent Advances in Renal Physiology; Wirz, H., Spinelli, F., Eds.; Karger Press: Basel, Switzerland, 1972; pp. 162-171.