Isolation of human neutrophils from venous blood

Methods in Molecular Biology

Volumn 1124, Issue , 2014, Pages 13-18

  Nauseef, William M ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

Dextran sedimentation; Ficoll Hypaque; Granulocytes; Mononuclear cells

Indexed keywords

ARTICLE; CELL DENSITY; CELL ISOLATION; CELL POPULATION; CELL SEPARATION; CONTROLLED STUDY; ERYTHROCYTE; EX VIVO STUDY; HUMAN; HUMAN CELL; LEUKOCYTE; MOLECULAR WEIGHT; MONOCYTE; NEUTROPHIL; PRIORITY JOURNAL; RELATIVE DENSITY; ROOM TEMPERATURE; VENOUS BLOOD;

CELL SEPARATION; HUMANS; NEUTROPHILS;

EID: 84899015437     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-845-4_2     Document Type: Article

References (4)

1. Böyum A (1968) Isolation of mononuclear cells and granulocytes from human blood: isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl 97:77-89
2. Skoog WA, Beck WS (1956) Studies on the fi brinogen, dextran, and phytohemagglutinin methods of isolating leukocytes. Blood 11:436-454
3. Stie J, Jesaitis AJ (2007) Reorganization of the human neutrophil plasma membrane is associated with functional priming: implications for neutrophil preparations. J Leukoc Biol 81:672-685
4. Nauseef WN (2007) Isolation of human neutrophils from venous blood. Methods Mol Biol 412:15-20