Filopodial morphology correlates to the capture efficiency of primary T-cells on nanohole arrays

Journal of Biomedical Nanotechnology

Volumn 10, Issue 6, 2014, Pages 1030-1040

  Kim, Dong Joo ^b   Kim, Gil Sung ^b   Seol, Jin Kyeong ^b   Hyung, Jung Hwan ^b   Park, No Won ^a   Lee, Mi Ri ^b   Lee, Myung Kyu ^d   Fan, Rong ^c,e   Lee, Sang Kwon ^a  

^a Chung Ang University   (South Korea)

^b Chonbuk National University   (South Korea)

^c Yale University   (United States)

^d Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^e YALE CANCER CENTER   (United States)

Author keywords

CD4 T lymphocytes; Cell capture and separation; Contact guidance; Filopodia; Quartz nanohole arrays; Streptavidin functionalization

Indexed keywords

CELL CULTURE; CELL MEMBRANES; CELL PROLIFERATION; MECHANICS; NANOSTRUCTURED MATERIALS; NANOSTRUCTURES; PROTEINS; QUARTZ; SEPARATION; T-CELLS;

CD4 T-LYMPHOCYTES; CELL CAPTURE; CONTACT GUIDANCE; FILOPODIA; FUNCTIONALIZATIONS; NANOHOLE ARRAYS;

EFFICIENCY;

STREPTAVIDIN;

ARTICLE; CD4+ T LYMPHOCYTE; CELL POPULATION; CELL SEPARATION; CELL SPREADING; CELL STRUCTURE; CELL SURFACE; FILOPODIUM; IMMUNOCOMPETENT CELL; LAMELLIPODIUM; MICROVILLUS; NANOANALYSIS; NANOARRAY; NANOFABRICATION; NANOHOLE ARRAY; NONHUMAN; SCANNING ELECTRON MICROSCOPE; T LYMPHOCYTE;

ANIMALS; CELLS, CULTURED; MATERIALS TESTING; MICE; MICE, INBRED C57BL; MOLECULAR CONFORMATION; NANOPARTICLES; NANOPORES; PARTICLE SIZE; POLYSTYRENES; SURFACE PROPERTIES; T-LYMPHOCYTES;

EID: 84898475178     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2014.1814     Document Type: Article

References (35)

1. A. Daser, P. Parronchi, E. Maggi, U. Wahn, S. Romagnani, and H. Renz, Allergen-specific T cell lines show restricted TCR V beta usage. human immunology. Hum. Immunol. 47, P875 (1996).
2. S. Romagnani, The role of T-cell derived cytokines in allergic diseases. Expert Opin. Ther. Pat. 6, 223 (1996).
3. X. H. Cheng, D. Irimia, M. Dixon, K. Sekine, U. Demirci, L. Zamir, R. G. Tompkins, W. Rodriguez, and M. Toner, A microfluidic device for practical label-free CD4+ T cell counting of HIV-infected subjects. Lab. Chip 7, 170 (2007).
4. J. W. Murphy, M. R. Hidore, and S. C. Wong, Direct interactions of human-lymphocytes with the yeast-like organism, cryptococcus-neoformans. J. Clin. Invest. 91, 1553 (1993).
5. D. M. Phillips and T. Xin, Hiv-1 infection of the trophoblast cell-line bewo - a study of virus uptake. AIDS Res. Hum. Retrov. 8, 1683 (1992)
6. M. C. Mingari, F. Schiavetti, M. Ponte, C. Vitale, E. Maggi, S. Romagnani, J. Demarest, G. Pantaleo, A. S. Fauci, and L. Moretta, Human CD8(+) T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations. Proc. Natl. Acad. Sci. USA 93, 12433 (1996).
7. R. Pearcepratt and D. M. Phillips, Studies of adhesion of lymphocytic cells - implications for sexual transmission of human-immunodeficiency-virus. Biol. Reprod. 48, 431 (1993).
8. K. G. E. Scott, L. C. H. Yu, and A. G. Buret, Role of CD8(+) and CD4(+) T lymphocytes in jejunal mucosal injury during murine giardiasis. Infect. Immun. 72, 3536 (2004).
9. G. W. Wolkersdorfer, T. Lohmann, C. Marx, S. Schroder, R. Pfeiffer, H. D. Stahl, W. A. Scherbaum, G. P. Chrousos, and S. R. Bornstein, Lymphocytes stimulate dehydroepiandrosterone production through direct cellular contact with adrenal zona reticularis cells: A novel mechanism of immune-endocrine interaction. J. Clin. Endocr. Metab. 84, 4220 (1999).
10. H. Cantor, E. Simpson, V. L. Sato, C. Garrison, and L. A. Herzenberg, Characterization of subpopulations of T-lymphocytes. 1. separation and functional studies of peripheral T-cells binding different amounts of fluorescent Anti-Thy 1.2 (Theta) antibody using a fluorescence-activated cell sorter (FACS). Cell Immunol. 15, 180 (1975).
11. K. Wang, B. Cometti, and D. Pappas, Isolation and counting of multiple cell types using an affinity separation. Anal. Chim. Acta 601, 1 (2007).
12. D. Huh, H. H. Wei, O. D. Kripfgans, J. B. Fowlkes, J. B. Grotberg, and S. Takayama (eds.), Gravity-driven microhydrodynamics-based cell sorter (microHYCS) for rapid, inexpensive, and efficient cell separation and size-profiling. Proceedings of the 2nd IEEE-EMBS Special Topics Conference on Microtechnologies in Medicine and Biology, Madison, WI, USA, May (2002).
13. Z. G. Wu, A. Q. Liu, and K. Hjort, Microfluidic continuous particle/cell separation via electroosmotic-flow-tuned hydrodynamic spreading. J. Micromech. Microeng. 17, 1992 (2007).
14. S. M. Prattis, D. H. Gebhart, G. Dickson, D. J. Watt, and J. N. Kornegay, Magnetic affinity cell sorting (Macs) separation and flow cytometric characterization of neural cell-adhesion molecule-positive, cultured myogenic cells from normal and dystrophic dogs. Exp. Cell Res. 208, 453 (1993).
15. B. Schmitz, A. Radbruch, T. Kummel, C. Wickenhauser, H. Korb, M. L. Hansmann, J. Thiele, and R. Fischer, Magnetic activated cell sorting (MACS) - a new immunomagnetic method for megakaryocytic cell isolation - comparison of different separation techniques. Eur. J. Haematol. 52, 267 (1994).
16. S. J. Qi, C. Q. Yi, S. L. Ji, C. C. Fong, and M. S. Yang, Cell adhesion and spreading behavior on vertically aligned silicon nanowire array. ACS Appl. Mater. Inter. 1, 30 (2009).
17. K. W. Kwon, S. S. Choi, S. H. Lee, B. Kim, S. N. Lee, M. C. Park, P. Kim, S. Y. Hwang, and K. Y. Suh, Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference. Lab. Chip 7, 1461 (2007).
18. K. Kulangara and K. W. Leong, Substrate topography shapes cell function. Soft Matter. 5, 4072 (2009).
19. S. T. Kim, D. J. Kim, T. J. Kim, D. W. Seo, T. H. Kim, S. Y. Lee, K. Kim, K. M. Lee, and S. K. Lee, Novel streptavidin-functionalized silicon nanowire arrays for CD4(+) T lymphocyte separation. Nano Lett. 10, 2877 (2010).
20. S. T. Wang, H. Wang, J. Jiao, K. J. Chen, G. E. Owens, K. I. Kamei, J. Sun, D. J. Sherman, C. P. Behrenbruch, H. Wu, and H. R. Tseng, Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells. Angew. Chem. Int. Edit. 48, 8970 (2009).
21. S. T. Wang, K. Liu, J. A. Liu, Z. T. F. Yu, X. W. Xu, L. B. Zhao, T. Lee, E. K. Lee, J. Reiss, Y. K. Lee, L. W. K. Chung, J. T. Huang, M. Rettig, D. Seligson, K. N. Duraiswamy, C. K. F. Shen, and H. R. Tseng, Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers. Angew. Chem. Int. Edit. 50, 3084 (2011).
22. D. J. Kim, J. K. Seol, Y. Wu, S. Ji, G. S. Kim, J. H. Hyung, S. Y. Lee, H. Lim, R. Fan, and S. K. Lee, A quartz nanopillar hemocytometer for high-yield separation and counting of CD4(+) T lymphocytes. Nanoscale 4, 2500 (2012).
23. W. Q. Chen, S. N. Weng, F. Zhang, S. Allen, X. Li, L. W. Bao, R. H. W. Lam, J. A. Macoska, S. D. Merajver, and J. P. Fu, Nanoroughened surfaces for efficient capture of circulating tumor cells without using capture antibodies. ACS Nano 7, 566 (2013).
24. W. Q. Chen, Y. B. Sun, and J. P. Fu, Microfabricated nanotopological surfaces for study of adhesion-dependent cell Mechanosensitivity. Small 9, 81 (2013).
25. P. Otteskog and K. G. Sundqvist, Characterization of the spreading process in human lymphocytes-T. Exp. Cell Res. 149, 201 (1983).
26. P. K. Mattila and P. Lappalainen, Filopodia: Molecular architecture and cellular functions. Nat. Rev. Mol. Cell Bio. 9, 446 (2008).
27. D. J. Kim, J. K. Seol, G. H. Lee, G. S. Kim, and S. K. Lee, Cell adhesion and migration on nanopatterned substrates and their effects on cell-capture yield. Nanotechnology 23, 395102 (2012).
28. C. J. Bettinger, R. Langer, and J. T. Borenstein, Engineering substrate topography at the micro- and nanoscale to control cell function. Angew. Chem. Int. Edit. 48, 5406 (2009).
29. M. J. Dalby, Topographically induced direct cell mechanotransduction. Med. Eng. Phys. 27, 730 (2005).
30. M. J. Dalby, N. Gadegaard, M. O. Riehle, C. D. W. Wilkinson, and A. S. G. Curtis, Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size. Int. J. Biochem. Cell B 36, 2005 (2004).
31. A. Hart, N. Gadegaard, C. D. W. Wilkinson, R. O. C. Oreffo, and M. J. Dalby, Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography. J. Mater. Sci.-Mater. M 18, 1211 (2007).
32. M. J. Dalby, M. O. Riehle, H. J. H. Johnstone, S. Affrossman, and A. S. G. Curtis, Polymer-demixed nanotopography: Control of fibroblast spreading and proliferation. Tissue Eng. 8, 1099 (2002).
33. U. Hersel, C. Dahmen, and H. Kessler, RGD modified polymers: Biomaterials for stimulated cell adhesion and beyond. Biomaterials 24, 4385 (2003).
34. F. Haq, V. Anandan, C. Keith, and G. Zhang, Neurite development in PC12 cells cultured on nanopillars and nanopores with sizes comparable with filopodia. Int. J. Nanomed. 2, 107 (2007).
35. S. Majstoravich, J. Y. Zhang, S. Nicholson-Dykstra, S. Linder, W. Friedrich, K. A. Siminovitch, and H. N. Higgs, Lymphocyte microvilli are dynamic, actin-dependent structures that do not require Wiskott-Aldrich syndrome protein (WASp) for their morphology. Blood 104, 1396 (2004).