Identification of a novel plasmid-associated spectinomycin adenyltransferase gene spd in methicillin-resistant staphylococcus aureus ST398 isolated from animal and human sources

Journal of Antimicrobial Chemotherapy

Volumn 69, Issue 5, 2014, Pages 1193-1196

  Jamrozy, D M ^a,b   Coldham, N G ^a   Butaye, P ^c,d   Fielder, M D ^b  

^a VETERINARY LABORATORIES AGENCY   (United Kingdom)

^b KINGSTON UNIVERSITY   (United Kingdom)

^c GHENT UNIVERSITY   (Belgium)

^d VETERINARY AND AGROCHEMICAL RESEARCH CENTRE   (Belgium)

Author keywords

Aminoglycosides; Antimicrobial resistance; MRSA

Indexed keywords

PLASMID DNA; SPECTINOMYCIN; BACTERIAL DNA; NUCLEOTIDYLTRANSFERASE; STREPTOMYCIN 3''-ADENYLYLTRANSFERASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATION; DNA ISOLATION; GENE IDENTIFICATION; GENE INACTIVATION; GENE SEQUENCE; HETEROZYGOTE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR SIZE; NONHUMAN; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION; REC GENE; REPN GENE; SEQUENCE ANALYSIS; SPD GENE; SPW GENE; ANIMAL; BACTERIUM TRANSFORMATION; CHEMISTRY; CLASSIFICATION; DNA SEQUENCE; ENZYMOLOGY; GENETICS; GENOTYPE; HORIZONTAL GENE TRANSFER; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR GENETICS; MOLECULAR TYPING; STAPHYLOCOCCUS INFECTION; VETERINARY;

ANIMALS; DNA, BACTERIAL; GENE TRANSFER, HORIZONTAL; GENOTYPE; HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR SEQUENCE DATA; MOLECULAR TYPING; NUCLEOTIDYLTRANSFERASES; PLASMIDS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; STAPHYLOCOCCAL INFECTIONS; TRANSFORMATION, BACTERIAL;

EID: 84898433331     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkt510     Document Type: Article

References (12)

1. Huijsdens XW, van Dijke BJ, Spalburg E et al. Community-acquired MRSA and pig-farming. Ann Clin Microbiol Antimicrob 2006; 5: 26.
2. Van den Eede A, Martens A, Lipinska U et al. High occurrence of methicillin-resistant Staphylococcus aureus ST398 in equine nasal samples. Vet Microbiol 2009; 133: 138-44.
3. Verhegghe M, Pletinckx L, Crombé F et al. Methicillin-resistant Staphylococcus aureus (MRSA) ST398 in pig farms and multispecies farms. Zoonoses Public Health 2013; 60: 366-74.
4. Kadlec K, Ehricht R, Monecke S et al. Diversity of antimicrobial resistance pheno-and genotypes of methicillin-resistant Staphylococcus aureus ST398 from diseased swine. J Antimicrob Chemother 2009; 64: 1156-64.
5. Overesch G, Buttner S, Rossano A et al. The increase of methicillin-resistant Staphylococcus aureus (MRSA) and the presence of an unusual sequence type ST49 in slaughter pigs in Switzerland. BMC Vet Res 2011; 7: 30.
6. Jamrozy DM, Fielder MD, Butaye P et al. Comparative genotypic and phenotypic characterisation of methicillin-resistant Staphylococcus aureus ST398 isolated from animals and humans. PLoS One 2012; 7: e40458.
7. Wendlandt S, Li B, Lozano C et al. Identification of the novel spectinomycin resistance gene spw in methicillin-resistant and methicillin-susceptible Staphylococcus aureus of human and animal origin. J Antimicrob Chemother 2013; 68: 1679-80.
8. McNamara PJ. Genetic manipulation of Staphylococcus aureus. In: Lindsay JA, ed. Staphylococcus: Molecular Genetics. Caister Academic Press, 2008; 89.
9. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual, Vol. 1. Cold Spring Harbor Laboratory Press, 2001.
10. Committee for Veterinary Medical Products. Spectinomycin, Summary Report. http://www.ema.europa.eu/docs/en_GB/document_library/ Maximum_Residue_Limits_-_Report/2009/11/WC500015987.pdf (2 September 2013, date last accessed).
11. LeBlanc D, Lee L, Inamine J. Cloning and nucleotide base sequence analysis of a spectinomycin adenyltransferase AAD(9) determinant from Enterococcus faecalis. Antimicrob Agents Chemother 1991; 35: 1804-10.
12. Werckenthin C, Cardoso M, Martel JL et al. Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius. Vet Res 2001; 32: 341-62.