An automated image processing routine for segmentation of cell cytoplasms in high-resolution autofluorescence images

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 8948, Issue , 2014, Pages

  Walsh, Alex J ^a   Skala, Melissa C ^a  

^a VANDERBILT UNIVERSITY   (United States)

Author keywords

fluorescence lifetime; image analysis; Microscopy

Indexed keywords

CELL CULTURE; CELL PROLIFERATION; DIAGNOSIS; DISEASES; FLUORESCENCE; IMAGE ANALYSIS; MICROSCOPIC EXAMINATION;

AUTOMATED IMAGE PROCESSING; CANCER DIAGNOSTICS; DISEASE PROGRESSION; FLUORESCENCE LIFETIMES; FLUORESCENCE SIGNALS; MICROSCOPIC IMAGE; SINGLE-CELL LEVEL; THERAPY MONITORING;

IMAGE SEGMENTATION;

EID: 84898047124     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.2040644     Document Type: Conference Paper

References (7)

1. P. Eroles, A. Bosch, B. Bermejo and A. Lluch, "Mechanisms of resistance to hormonal treatment in breast cancer," Clin Transl Oncol 12(4), 246-252 (2010)
2. J. W. Locasale, "Metabolic rewiring drives resistance to targeted cancer therapy," Mol Syst Biol 8(597 (2012)
3. M. Al-Hajj, M. S. Wicha, A. Benito-Hernandez, S. J. Morrison and M. F. Clarke, "Prospective identification of tumorigenic breast cancer cells," Proc Natl Acad Sci U S A 100(7), 3983-3988 (2003) (Pubitemid 36418143)
4. M. C. Skala, K. M. Riching, D. K. Bird, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, P. J. Keely and N. Ramanujam, "In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia," J Biomed Opt 12(2), 024014 (2007)
5. M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White and N. Ramanujam, "In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia," Proc Natl Acad Sci U S A 104(49), 19494-19499 (2007)
6. A. J. Walsh, R. S. Cook, H. C. Manning, D. J. Hicks, A. Lafontant, C. L. Arteaga and M. C. Skala, "Optical metabolic imaging identifies glycolytic levels, subtypes, and early-treatment response in breast cancer," Cancer Res 73(20), 6164-6174 (2013)
7. A. Walsh, R. S. Cook, B. Rexer, C. L. Arteaga and M. C. Skala, "Optical imaging of metabolism in HER2 overexpressing breast cancer cells," Biomed Opt Express 3(1), 75-85 (2012)