Impact of library preparation on downstream analysis and interpretation of RNA-Seq data: comparison between Illumina PolyA and NuGEN Ovation protocol.

PloS one

Volumn 8, Issue 8, 2013, Pages

  Sun, Zhifu ^a   Asmann, Yan W ^b   Nair, Asha ^b   Zhang, Yuji ^b   Wang, Liguo ^b   Kalari, Krishna R ^b   Bhagwate, Aditya V ^b   Baker, Tiffany R ^b   Carr, Jennifer M ^b   Kocher, Jean Pierre A ^b   Perez, Edith A ^b   Thompson, E Aubrey ^b  

^a Mayo Clinic College of Medicine

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

LONG UNTRANSLATED RNA; MESSENGER RNA; POLYADENYLIC ACID;

CELL LINE; COMPARATIVE STUDY; EPITHELIUM CELL; EXON; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETICS; HUMAN; METABOLISM; PROCEDURES; REPRODUCIBILITY; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; STATISTICAL ANALYSIS; STATISTICS;

CELL LINE; DATA INTERPRETATION, STATISTICAL; EPITHELIAL CELLS; EXONS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE LIBRARY; HUMANS; POLY A; POLYMORPHISM, SINGLE NUCLEOTIDE; REPRODUCIBILITY OF RESULTS; RNA, LONG NONCODING; RNA, MESSENGER; SEQUENCE ANALYSIS, RNA; STATISTICS AS TOPIC;

EID: 84897927641     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0071745     Document Type: Article

References (0)