Major pesticides are more toxic to human cells than their declared active principles

BioMed Research International

Volumn 2014, Issue , 2014, Pages

  Mesnage, Robin ^a   Defarge, Nicolas ^a   Spiroux De Vendômois, Joël ^b   Séralini, Gilles Eric ^a  

^a UNIVERSITÉ DE CAEN   (France)

^b CRIIGEN   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ACETAMIPRID; CASPASE 3; CASPASE 7; EPOXICONAZOLE; FLUROXYPYR; FUNGICIDE; GLYPHOSATE; HERBICIDE; IMIDACLOPRID; INSECTICIDE; ISOPROTURON; PESTICIDE; PIRIMICARB; PROCHLORAZ; TEBUCONAZOLE; UNCLASSIFIED DRUG; CASPASE;

ARTICLE; CELL STRAIN; CELL STRAIN HEK293; CELL STRAIN HEPG2; CELL STRAIN JEG3; CELL TYPE; CONTROLLED STUDY; CYTOTOXICITY; ENVIRONMENTAL EXPOSURE; ENZYME ACTIVATION; ENZYME ACTIVITY; HEPATOMA CELL; HUMAN; HUMAN CELL; MEMBRANE DAMAGE; BIOSYNTHESIS; CELL MEMBRANE; DOSE RESPONSE; DRUG EFFECTS; HEK293 CELL LINE; HEPG2 CELL LINE; MITOCHONDRION; TOXICITY;

CASPASES; CELL MEMBRANE; DOSE-RESPONSE RELATIONSHIP, DRUG; ENVIRONMENTAL EXPOSURE; HEK293 CELLS; HEP G2 CELLS; HUMANS; MITOCHONDRIA; PESTICIDES;

EID: 84897847011     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2014/179691     Document Type: Article

References (41)

1. Mesnage R., Bernay B., Seralini G. E., Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity. Toxicology 2013 313 2-3 122 128 10.1016/j.tox.2012.09.006
2. Seralini G. E., Mesnage R., Defarge N., Answers to critics: why there is a long term toxicity due to NK603 Roundup-tolerant genetically modified maize and to a Roundup herbicide. Food and Chemical Toxicology 2013 53 461 468
3. Gasnier C., Dumont C., Benachour N., Clair E., Chagnon M., Séralini G., Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines. Toxicology 2009 262 3 184 191 2-s2.0-67651014408 10.1016/j.tox.2009.06.006
4. Peluso M., Munnia A., Bolognesi C., Parodi S., 32P-postlabeling detection of DNA adducts in mice treated with the herbicide Roundup. Environmental and Molecular Mutagenesis 1998 31 1 55 59 (Pubitemid 28080817)
5. Walsh L. P., McCormick C., Martin C., Stocco D. M., Roundup inhibits steroidogenesis by disrupting steroidogenic acute regulatory (StAR) protein expression. Environmental Health Perspectives 2000 108 8 769 776 2-s2.0-0033790179
6. Bradberry S. M., Proudfoot A. T., Vale J. A., Glyphosate poisoning. Toxicological Reviews 2004 23 3 159 167 2-s2.0-17544379407 10.2165/00139709- 200423030-00003 (Pubitemid 40553034)
7. Adam A., Marzuki A., Rahman H. A., Aziz M. A., The oral and intratracheal toxicities of ROUNDUP and its components to rats. Veterinary and Human Toxicology 1997 39 3 147 151 2-s2.0-1842403589 (Pubitemid 27215102)
8. European Commission, The use of plant protection products in the European Union. 2007, http://epp.eurostat.ec.europa.eu/
9. US EPA, Pesticide Industry Sales and Usage. 2012, http://www.epa.gov/ opp00001/pestsales/
10. Székács A., Darvas B., Hasaneen M. N. A. E.-G., Forty years with glyphosate. Herbicides-Properties, Synthesis and Control of Weeds 2012 Rijeka, Croatia InTech
11. Commissariat Général au Développement Durable, Les Pesticides Dans Les Milieux Aquatiques 2010 Paris, France Études et Documents
12. Durkin P. R., Fluroxypyr human health and ecological risk assessment report. SERA 2009 26 TR-052-13-03a Washington, DC, USA USDA Forest service
13. Vera Candioti J., Natale G. S., Soloneski S., Ronco A. E., Larramendy M. L., Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida® Chemosphere 2010 78 3 249 255 2-s2.0-71849102009 10.1016/j.chemosphere.2009.10.064
14. Ambrose M. L., Characterization of the Insecticidal Properties of Acetamiprid Under Field and Laboratory Conditions 2003 Raleigh, NC, US Faculty of North Carolina State University
15. Dicks L., Bees, lies and evidence-based policy. Nature 2013 494 7437 283 10.1038/494283a
16. Aliouane Y., El Hassani A. K., Gary V., Armengaud C., Lambin M., Gauthier M., Subchronic exposure of honeybees to sublethal doses of pesticides: effects on behavior. Environmental Toxicology and Chemistry 2009 28 1 113 122 2-s2.0-65449170134 10.1897/08-110.1
17. Agropages, Triazole Fungicides Insight-Market overview. 2013, http://www.agropages.com/BuyersGuide/category/Triazole-Fungicides-Insight. html#smmn1
18. Letcher R. J., Van Holsteijn I., Drenth H., Norstrom R. J., Bergman Å., Safe S., Pieters R., Van Den Berg M., Cytotoxicity and aromatase (CYP19) activity modulation by organochlorines in human placental JEG-3 and JAR choriocarcinoma cells. Toxicology and Applied Pharmacology 1999 160 1 10 20 2-s2.0-0033214715 10.1006/taap.1999.8746 (Pubitemid 29489759)
19. Urani C., Doldi M., Crippa S., Camatini M., Human-derived cell lines to study xenobiotic metabolism. Chemosphere 1998 37 14-15 2785 2795 2-s2.0-0032402090 10.1016/S0045-6535(98)00321-X (Pubitemid 28522003)
20. Kuiper G. G., Lemmen J. G., Carlsson B., Corton J. C., Safe S. H., Van Der Saag P. T., Van Der Burg B., Gustafsson J., Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor β Endocrinology 1998 139 10 4252 4263 2-s2.0-0031733298 (Pubitemid 28513713)
21. Krijt J., Van Holsteijn I., Hassing I., Vokurka M., Blaauboer B. J., Effect of diphenyl ether herbicides and oxadiazon on porphyrin biosynthesis in mouse liver, rat primary hepatocyte culture and HepG2 cells. Archives of Toxicology 1993 67 4 255 261 2-s2.0-0027271822 (Pubitemid 23131832)
22. Nakagawa I., Suzuki M., Imura N., Naganuma A., Enhancement of paraquat toxicity by glutathione depletion in mice in vivo and in vitro. Journal of Toxicological Sciences 1995 20 5 557 564 2-s2.0-0029561609 (Pubitemid 26035207)
23. Richard S., Moslemi S., Sipahutar H., Benachour N., Seralini G., Differential effects of glyphosate and roundup on human placental cells and aromatase. Environmental Health Perspectives 2005 113 6 716 720 2-s2.0-20844460307 10.1289/ehp.7728 (Pubitemid 40862183)
24. L'Azou B., Fernandez P., Bareille R., Beneteau M., Bourget C., Cambar J., Bordenave L., In vitro endothelial cell susceptibility to xenobiotics: comparison of three cell types. Cell Biology and Toxicology 2005 21 2 127 137 2-s2.0-24344505905 10.1007/s10565-005-0172-8 (Pubitemid 41253205)
25. Benachour N., Séralini G., Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells. Chemical Research in Toxicology 2009 22 1 97 105 2-s2.0-61849171735 10.1021/tx800218n
26. Korting H. C., Schindler S., Hartinger A., Kerscher M., Angerpointner T., Maibach H. I., MTT-assay and neutral red release (NRR)-assay: relative role in the prediction of the irritancy potential of surfactants. Life Sciences 1994 55 7 533 540 2-s2.0-0028206471 10.1016/0024-3205(94)00746-2 (Pubitemid 24230340)
27. Liu J. J., Wang W., Dicker D. T., El-Deiry W. S., Bioluminescent imaging of TRAIL-induced apoptosis through detection of caspase activation following cleavage of DEVD-aminoluciferin. Cancer Biology & Therapy 2005 4 8 885 892 2-s2.0-33745531312
28. Crouch S. P., Kozlowski R., Slater K. J., Fletcher J., The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. Journal of Immunological Methods 1993 160 1 81 88 2-s2.0-0027499070 10.1016/0022-1759(93) 90011-U
29. Benachour N., Sipahutar H., Moslemi S., Gasnier C., Travert C., Séralini G. E., Time- and dose-dependent effects of roundup on human embryonic and placental cells. Archives of Environmental Contamination and Toxicology 2007 53 1 126 133 2-s2.0-34249828181 10.1007/s00244-006-0154-8 (Pubitemid 46852853)
30. Mosmann T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 1983 65 1-2 55 63 2-s2.0-0021061819 (Pubitemid 14203433)
31. Schneider C. A., Rasband W. S., Eliceiri K. W., NIH Image to ImageJ: 25 years of image analysis. Nature Methods 2012 9 7 671 675 10.1038/nmeth.2089
32. Marutani M., Edirveerasingam V., Influence of irrigation methods and an adjuvant on the persistence of carbaryl on pakchoi. Journal of Environmental Quality 2006 35 6 1994 1998 2-s2.0-33845295777 10.2134/jeq2005.0484 (Pubitemid 44870620)
33. McKee R. H., Wong Z. A., Schmitt S., Beatty P., Swanson M., Schreiner C. A., Schardein J. L., The reproductive and developmental toxicity of high flash aromatic naphtha. Toxicology and Industrial Health 1990 6 3-4 441 460 2-s2.0-0025050608 (Pubitemid 20301884)
34. Langman J. M., Xylene: its toxicity, measurement of exposure levels, absorption, metabolism and clearance. Pathology 1994 26 3 301 309 2-s2.0-0028477406
35. Saillenfait A. M., Gallissot F., Langonné I., Sabaté J. P., Developmental toxicity of N-methyl-2-pyrrolidone administered orally to rats. Food and Chemical Toxicology 2002 40 11 1705 1712 2-s2.0-0036051777 10.1016/S0278-6915(02)00115-1
36. US Environmental Protection Agency (EPA), Hazard Characterization Document-N, N-Dimethylalkanamides Category. 2011, http://www.epa.gov/chemrtk/ hpvis/hazchar/Category-N,N-Dimethylalkanamides-September-2011.pdf
37. US Environmental Protection Agency (EPA), Reregistration Eligibility Decision (RED) For Benzisothiazoline-3-One 2005 Boston, Mass, USA US Environmental Protection Agency (EPA)
38. Gawade L., Dadarkar S. S., Husain R., Gatne M., A detailed study of developmental immunotoxicity of imidacloprid in Wistar rats. Food and Chemical Toxicology 2013 51 61 70 10.1016/j.fct.2012.09.009
39. Williams G. M., Kroes R., Munro I. C., Safety evaluation and risk assessment of the herbicide Roundup and its active ingredient, glyphosate, for humans. Regulatory Toxicology and Pharmacology 2000 31 2, part 1 117 165 2-s2.0-0034070443 10.1006/rtph.1999.1371 (Pubitemid 30314343)
40. Williams A. L., Watson R. E., Desesso J. M., Developmental and reproductive outcomes in humans and animals after glyphosate exposure: a critical analysis. Journal of Toxicology and Environmental Health B 2012 15 1 39 96 2-s2.0-84856296635 10.1080/10937404.2012.632361
41. European Environment Agency, Late lessons from early warnings: science, precaution, innovation. EEA Report 2013 Copenhagen, Denmark European Environment Agency