Establishment of an untransfected human corneal epithelial cell line and its biocompatibility with denuded amniotic membrane

International Journal of Ophthalmology

Volumn 4, Issue 3, 2011, Pages 228-234

  Fan, Ting Jun ^a   Xu, Bin ^a   Zhao, Jun ^a   Yang, Hong Shou ^a   Wang, Rui Xin ^a   Hu, Xiu Zhong ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

Biocompatibility; Cell line; Denuded amniotic membrane; Human corneal epithelial cell; Untransfected

Indexed keywords

EID: 84897584493     PISSN: 22223959     EISSN: 22274898     Source Type: Journal    
DOI: 10.3980/j.issn.2222-3959.2011.03.02     Document Type: Article

References (23)

1. Kinoshita S, Adachi W, Sotozono C., Nishida K, Yokoi N, Quantock A.J., Okubo K. Characteristics of the Human Ocular Surface Epithelium. Prog Retin Eye Res. 2001; 20(5):639-673.
2. Dua HS, Shanmuganathan VA, Powell-Richardsa O, Tighe P.J., Joseph A. Limbal epithelial crypts: a novel anatomical structure and a putative limbal stem cell niche. Br J Ophthalmol. 2005; 89(5):529-532.
3. Stepp MA, Zieske JD The corneal epithelial stem cell niche. Ocul Surf. 2005; 3(1):15-26.
4. Daniels JT, Notara M, Shortta J., Secker G, Harris A, Tuft SJ Limbal epithelial stem cell therapy. Expert Opin Biol Ther. 2007; 7(1):1-3.
5. Notara M, Alatza A, Gilfillan J., Harris AR, Levis HJ, Schrader S, Vernon A., Daniels JT In sickness and in health: Corneal epithelial stem cell biology, pathology and therapy. Exp Eye Res. 2010; 90(2):188-195.
6. Shortta J, Secker GA, Notara M.D., Limb GA, Khaw PT, Tuft SJ, Daniels JT Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results. Surv Ophthalmol. 2007; 52(5):483-502.
7. Andri KR, Roger WB, Laurence S.L., Jodhbir SM Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction. Biom. 2010; 31(2):216-225.
8. Fan TJ, Yang HS, Hu X.Z., Zhao J. Research advances on in vitro reconstruction of tissue-engineered human corneal epithelium. J Shandong Univ Med. 2010; 48(7):7-13.
9. Federico CM. Corneal epithelial cell cultures as a tool for research, drug screening and testing. Exp Eye Res. 2008; 86:459-469.
10. Liu J, Song G, Wang Z., Huang B, Gao Q, Liu B., Xu Y, Liang X, Ma P., Gao N, Ge J. Establishment of a corneal epithelial cell line spontaneously derived from human limbal cells. Exp Eye Res. 2007; 84(3):599-609.
11. Araki-Sasaki K. An SV40-immortalized human corneal epithelial cell line and its characterization. Invest Ophthalmol Vis Sci. 1995; 36(3):614-621.
12. Yamasaki K, Kawasaki S, Young R.D., Fukuoka H., Tanioka H, Nakatsukasa M, Quantock A.J., Kinoshita S. Genomic Aberrations and Cellular Heterogeneity in SV40-Immortalized Human Corneal Epithelial Cells. Invest Ophthalmol Vis Sci. 2009; 50(2):604-613.
13. Robertson DM, Li L, Fisher S., Pearce VP, Shay JW, Wright WE, Cavanagh H.D., Jester JV Characterization of growth and differentiation in a telomerase-immortalized human corneal epithelial cell line. Invest Ophthalmol Vis Sci. 2005; 46(2):470-478.
14. Yamasaki K, Kawasaki S, Young R.D., Fukuoka H., Tanioka H, Nakatsukasa M, Quantock A.J., Kinoshita S. Genomic aberrations and cellular heterogeneity in SV40-immortalized human corneal epithelial cells. Invest Ophthalmol Vis Sci. 2009; 50(2):604-613.
15. Fan T, Zhao J, Ma X., Xu X, Zhao W, Xu B. Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane. Mol Vis. 2011; 17:469-480.
16. Fan T, Zhao J, Fu Y., Cong R, Guo R, Liu W., Han B, Yu Q, Wang J. Establishment of a novel corneal endothelial cell line from domestic rabbit, Oryctolagus curiculus. Sci China C. 2007; 50(2):161-169.
17. Fan T, Wang D, Zhao J., Wang J, Fu Y, Guo R. Establishment and characterization of a novel untransfected corneal endothelial cell line from New Zealand white rabbits. Mol Vis. 2009; 15:1070-1078.
18. Fan T, Zhao J, Ma X., Xu X, Zhao W, Xu B. Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane. Mol Vis. 2011; 17:469-480.
19. Kahn CR, Young E, Lee I.H., Rhim JS Human corneal epithelial primary cultures and cell lines with extended life span: in vitro model for ocular studies. Invest Ophthalmol Vis Sci. 1993; 234:3429-3441.
20. Schermer A, Galvin S, Sun TT Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. Cell Biol. 1986; 103:49-62.
21. Vrana NE, Builles N, Justin V., Bednarz J, Pellegrini G, Ferrari B., Damour O, Hulmes DJ, Hasirci V. Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures. Invest Ophthalmol Vis Sci. 2008; 49:5325-5331.
22. Shortta J, Secker GA, Rajan M.S., Meligonis G., Dart JK, Tuft SJ, Daniels JT Ex vivo expansion and transplantation of limbal epithelial stem cells. Ophthalmol. 2008; 115(11):1989-1997.
23. Nadia Z, Carina K, Viggo V T, Zwi B, Andrew H., Marie-José T. Standardized Limbal Epithelial Stem Cell Graft Generation and Transplantation. Tissue Eng C. 2010; 16(5):921-927.