Silencing the glycerol-3-phosphate dehydrogenase gene in Saccharomyces cerevisiae results in more ethanol being produced and less glycerol

Biotechnology Letters

Volumn 36, Issue 3, 2014, Pages 523-529

  He, Wenjin ^a,b   Ye, Shichao ^a,b   Xue, Ting ^a,b   Xu, Shengyan ^a,b   Li, Weiyan ^a,b   Lu, Jihua ^a,b   Cao, Luoyuan ^c   Ye, Bingying ^a,b   Chen, Youqiang ^a,b  

^a FUJIAN NORMAL UNIVERSITY   (China)

^b Fujian Key Laboratory of Developmental and Neuro Biology   (China)

^c Key Laboratory of Sugarcane Biology and Genetic Breeding of Ministry of Agriculture College of Life Sciences Fujian Normal University ^*

Author keywords

Ethanol; Glycerol; Glycerol 3 phosphate dehydrogenase; Overlap PCR; Saccharomyces cerevisiae

Indexed keywords

ETHANOL; GENES; STRAIN; TRANSCRIPTION; YEAST;

BIO-ETHANOLS; DEHYDROGENASE ACTIVITY; ETHANOL PRODUCTION; GLYCEROL PRODUCTION; GLYCEROL-3-PHOSPHATE DEHYDROGENASE; INDUSTRIAL STRAIN; OVERLAP PCR; S.CEREVISIAE;

GLYCEROL;

SACCHAROMYCES CEREVISIAE;

ALCOHOL; GLYCEROL; GLYCEROL 3 PHOSPHATE DEHYDROGENASE (NAD); GPD1 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; DRUG ANTAGONISM; ENZYMOLOGY; GENE SILENCING; GENETICS; METABOLISM; SACCHAROMYCES CEREVISIAE;

ETHANOL; GENE SILENCING; GLYCEROL; GLYCEROL-3-PHOSPHATE DEHYDROGENASE (NAD+); SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84897059880     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-013-1375-3     Document Type: Article

References (13)

1. Albertyn J, van Tonder A, Prior BA (1992) Purification and characterization of glycerol-3-phosphate dehydrogenase of Saccharomyces cerevisiae. FEBS Lett 308: 130-132.
2. Bonoli M, Graziola M, Poggi V et al (2006) RNA complementary to the 5′-UTR of mRNA triggers effective silencing in Saccharomyces cerevisiae. Biochem Biophys Res Commun 339: 1224-1231.
3. Brenet F, Dussault N, Delfino C et al (2006) Identification of secondary structure in the 5′-untranslated region of the human adrenomedullin mRNA with implications for the regulation of mRNA translation. Oncogene 25: 6510-6519.
4. Cannons AC, Cannon J (2002) The stability of the chlorella nitrate reductase mRNA is determined by the secondary structure of the 5′-UTR: implications for posttranscriptional regulation of nitrate reductase. Planta 214: 488-491.
5. Curie C, McCormick S (1997) A strong inhibitor of gene expression in the 5′ untranslated region of the pollen-specific LAT59 gene to tomato. Plant Cell 9: 2025-2036.
6. Ishida N, Suzuki T, Tokuhiro K et al (2006) D-Lactic acid production by metabolically engineered Saccharomyces cerevisiae. Biosci Bioeng 101: 172-177.
7. Kotaka A, Bando H, Kaya M et al (2008) Direct ethanol production from barley β-glucan by sake yeast displaying Aspergillus oryzae β-glucosidase and endoglucanase. Biosci Bioeng 105: 622-627.
8. Lee WJ, Choi BS (2013) Reliability and validity of soft copy images based on flat-panel detector in pneumoconiosis classification: comparison with the analog radiographs. Acad Radiol 20: 746-751.
9. Liu X, Li F, Chen H et al (2008) Advances in biocontrol of pathogenic Fusarium oxysporum. J Yunnan Univ 30: 86-88.
10. Nelson MD, Fitch DHA (2009) Overlap extension PCR: an efficient method for transgenic construction. Mol Methods Evolut Genet Methods Mol Biol 8: 13-186.
11. Song JZ, Yang XX, Wang Y, Yang Q (2009) Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana. J Harbin Institute of Technology 16: 200-204.
12. Thom PE, Trench RK (1993) Osmoregulation and the genetic induction of glycerol 3-phosphate dehydrogenase by NaCl in the euryhaline yeast debaryomyces hansenii. Biotechnol 1: 230-238.
13. Xu F, Yao Q, Xiong A et al (2006) SOE PCR and its application in genetic engineering. Mol Plant Breed 4: 747-750.