The optics inside an automated single molecule array analyzer

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 8935, Issue , 2014, Pages

  McGuigan, William ^a   Fournier, David R ^b   Watson, Gary W ^a   Walling, Les ^a   Gigante, Bill ^a   Duffy, David C ^b   Rissin, David M ^b   Kan, Cheuk W ^b   Meyer, Raymond E ^b   Piech, Tomasz ^b   Fishburn, Matthew W ^b  

^a Stratec Biomedical   (United States)

^b Quanterix ^*  (United States)

Author keywords

Automation; Digital ELISA; Microstructure arrays; Quanterix; Simoa; Single molecule imaging; Stratec Biomedical; Wide field of view fluorescence microscope

Indexed keywords

AUTOMATION; FLUORESCENCE; IMAGE PROCESSING; MICROSTRUCTURE; OPTICAL DATA PROCESSING; PROTEINS; REMOTE CONTROL; SURGERY;

DIGITAL ELISA; MICROSTRUCTURE ARRAYS; QUANTERIX; SIMOA; SINGLE-MOLECULE IMAGING; STRATEC BIOMEDICAL; WIDE FIELD-OF-VIEW;

MOLECULES;

EID: 84896976404     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.2041706     Document Type: Conference Paper

References (14)

1. Rissin, D. M.; Kan, C. W.; Campbell, T. G.; Howes, S. C.; Fournier, D. R.; Song, L.; Piech, T.; Patel, P. P.; Chang, L.; Rivnak, A. J.; Ferrell, E. P.; Randall, J. D.; Provuncher, G. K.; Walt, D. R.; Duffy, D. C. "Singlemolecule enzyme-linked immunosorbent assay detectes serum proteins at subfemtomolar concentrations," Nat. Biotechnol. 28, 595-599, (2010).
2. Rissin, D. M.; Fournier, D. R.; Piech, T.; Kan, C. W.; Campbell, T. G.; Song, L.; Chang, L.; Rivnak, A. J.; Patel, P. P.; Provuncher, G. K.; Ferrell, E. P.; Howes, S. C.; Pink, B. A.; Minnehan, K. A.; Wilson, D. H.; Duffy, D. C. "Simultaneous detcion of single molecules and singulated ensembles of molecules enables immunoassays with broad dynamic range," Anal. Chem. 83, 2279-2285, (2011).
3. Flusberg, B. A.; Webster, D. R.; Lee, J. H.; Travers, K. J.; Olivares, E. C.; Clark, T. A.; Korlach, J.; Turner, S. W. "Direct detection of DNA methylation during single-molecule, real-time sequencing." Nat. Methods 6, 461-465, (2010).
4. Pushkarev, D.; Neff, N. F.; Quake, S. R. "Single-molecule sequencing of an individual human genome." Nat. Biotechnol. 27, 847-852, (2009).
5. Taniguchi, Y.; Choi, P. J.; Li, G.-W.; Chen, H.; Babu, M.; Hearn, J.; Emili, A.; Xie, X. S. "Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells." Science 329, 533-538, (2010).
6. Luo, G.; Wang, M.; Konigsberg, W. H.; Xie, X. S. "Single-molecule and ensemble fluorescence assays for a functionally important conformational change in T7 DNA polymerase." Proc. Natl. Acad. Sci. USA 104, 12610-12615, (2007).
7. Tood, J.; Freese, B.; Lu, A.; Held, D.; Morey, J.; Livingston, R.; Goix, P. "Ultrasensitive flow-based immunoassays using single-molecule counting." Clin. Chem. 53 (11), 1990-1995, (2007).
8. Rotman, B. "Measurement of Activity of Single Molecule of b-D-Galactosidase," Proc. Natl. Acad. Sci U.S.A. 47, 1981-1991, (1961).
9. Rissin, D. M.; Walt, D. R. "Digital concentration readout of single enzyme molecules using femtoliter arrays and Poisson statistics," Nano Lett. 6, 520-523. (2006).
10. Rondelez Y, Tresser G, Tabata KV, Arata H, Fujita H, Takeuchi S, Noji H. "Microfabricated arrays of femtoliter chambers allow single molecule enzymology" Nat Biotechnol. 23(3),361-5. (2005)
11. Kan, C. W.; Rivnak A. J.; Campbell T. G.; Piech, T.; Rissin, D. M.; Mosl, M.; Peterca, A.; Niederberger, H.-P.; Minnehan, K. A.; Patel, P. P.; Ferrell, E. P.; Meyer, R. E.; Chang, L.; Wilson, D. H.; Fournier, D. R.; Duffy, D. C. "Isolation and detection of single molecules on paramagnetic beads using sequential fluid flows in microfabricated polymer array assemblies," Lab Chip, 12, 977-995, (2012)
12. Rissin, D. M.; Kan, C. W.; Song, L.; Rivnak, A. J.; Fishburn, M. W.; Shao, Q.; Piech, T.; Ferrell, E. P.; Meyer, R. E.; Campbell, T. C.; Fournier, D. R.; Duffy, D. C. "Multiplexed single molecule immunoassays," Lab Chip, 13, 2902-2911, (2013)
13. Wilson, D. H., Hanlon, D. W., Provuncher, G. K., Chang, L., Song, L., Patel, P. P., Ferrell, E. P., Lepor, H., Partin, A. W., Chan, D. W., Sokoll, L. J., Cheli, C. D., Thiel, R. P., Fournier, D. R., Duffy, D. C. "Fifth-Generation Digital Immunoassay for Prostate-Specific Antigen by Single Molecule Array Technology." Clin. Chem. 57, 1712-1721 (2011).
14. Wild, D., [The Immunoassay Handbook Fourth Edition], Gulf Professional Publishing; Chapter 2.13 (2013)