Gel filtration chromatography (size exclusion chromatography) of proteins

Methods in Enzymology

Volumn 541, Issue , 2014, Pages 105-114

  Duong Ly, Krisna C ^a   Gabelli, Sandra B ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Column volume; Gel filtration chromatography; Gel filtration column; Size exclusion chromatography; Void volume

Indexed keywords

PROTEIN; BUFFER; TROMETAMOL;

ANTIBODY AFFINITY; ARTICLE; GEL FILTRATION; GEL FILTRATION CHROMATOGRAPHY; METHODOLOGY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; STANDARDIZATION; CHEMISTRY; DEVICES; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; PROCEDURES;

BUFFERS; CHROMATOGRAPHY, GEL; PROTEINS; TROMETHAMINE;

EID: 84896960574     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-420119-4.00009-4     Document Type: Chapter

References (14)

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2. J.R. Whitaker Analytical Chemistry 35 1963 1950
3. P. Cutler Size-exclusion chromatography R. Rapley, J.M. Walker, Molecular Biomethods Handbook 1998 Humana Press 451 460
4. Protein Affinity Purification using Intein/Chitin Binding Protein Tags.
5. Purification of His-tagged proteins.
6. Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag.
7. Purification of GST-tagged proteins.
8. Immunoaffinity purification of proteins.
9. Strep-tagged protein purification.
10. Proteolytic affinity tag cleavage.
11. Hydroxyapatite Chromatography: Purification Strategies for Recombinant Proteins.
12. Using ion exchange chromatography to purify a recombinantly expressed protein.
13. TCA Precipitation.
14. Salting out of proteins using ammonium sulfate precipitation.